Phenolic compounds from Viscum album tinctures enhanced antitumor activity in melanoma murine cancer cells
Cancer is one of the biggest problems in public health worldwide. Plants have been shown important role in anticancer research. Viscum album L. (Santalaceae), commonly known as mistletoe, is a semi-parasitic plant that grows on different host trees. In complementary medicine, extracts from European...
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Format: | Article |
Language: | English |
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Elsevier
2018-03-01
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Series: | Saudi Pharmaceutical Journal |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016418300112 |
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doaj-e2c21b5994a744c3b1d02f35f248eca6 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michelle Nonato de Oliveira Melo Adriana Passos Oliveira Adalgisa Felippe Wiecikowski Renato Sampaio Carvalho Juliana de Lima Castro Felipe Alves Gomes de Oliveira Henrique Marcelo Gualberto Pereira Venicio Feo da Veiga Marcia Marques Alves Capella Leandro Rocha Carla Holandino |
spellingShingle |
Michelle Nonato de Oliveira Melo Adriana Passos Oliveira Adalgisa Felippe Wiecikowski Renato Sampaio Carvalho Juliana de Lima Castro Felipe Alves Gomes de Oliveira Henrique Marcelo Gualberto Pereira Venicio Feo da Veiga Marcia Marques Alves Capella Leandro Rocha Carla Holandino Phenolic compounds from Viscum album tinctures enhanced antitumor activity in melanoma murine cancer cells Saudi Pharmaceutical Journal Viscum album Mistletoe Lignans Phenolic compounds Antitumoral |
author_facet |
Michelle Nonato de Oliveira Melo Adriana Passos Oliveira Adalgisa Felippe Wiecikowski Renato Sampaio Carvalho Juliana de Lima Castro Felipe Alves Gomes de Oliveira Henrique Marcelo Gualberto Pereira Venicio Feo da Veiga Marcia Marques Alves Capella Leandro Rocha Carla Holandino |
author_sort |
Michelle Nonato de Oliveira Melo |
title |
Phenolic compounds from Viscum album tinctures enhanced antitumor activity in melanoma murine cancer cells |
title_short |
Phenolic compounds from Viscum album tinctures enhanced antitumor activity in melanoma murine cancer cells |
title_full |
Phenolic compounds from Viscum album tinctures enhanced antitumor activity in melanoma murine cancer cells |
title_fullStr |
Phenolic compounds from Viscum album tinctures enhanced antitumor activity in melanoma murine cancer cells |
title_full_unstemmed |
Phenolic compounds from Viscum album tinctures enhanced antitumor activity in melanoma murine cancer cells |
title_sort |
phenolic compounds from viscum album tinctures enhanced antitumor activity in melanoma murine cancer cells |
publisher |
Elsevier |
series |
Saudi Pharmaceutical Journal |
issn |
1319-0164 |
publishDate |
2018-03-01 |
description |
Cancer is one of the biggest problems in public health worldwide. Plants have been shown important role in anticancer research. Viscum album L. (Santalaceae), commonly known as mistletoe, is a semi-parasitic plant that grows on different host trees. In complementary medicine, extracts from European mistletoe (Viscum album L.) have been used in the treatment of cancer. The study was conducted to identify chemical composition and antitumor potential of Viscum album tinctures. Chemical analysis performed by high resolution chromatography equipped with high resolution mass spectrometer identified caffeic acid, chlorogenic acid, sakuranetin, isosakuranetin, syringenin 4-O-glucoside, syringenin 4-O-apiosyl-glucoside, alangilignoside C and ligalbumoside A compounds. Some of these compounds are probably responsible for the reduction of tumoral cellular growth in a dose-dependent manner. It was observed that melanoma murine cells (B16F10) were more sensitive to V. album tinctures than human leukaemic cells (K562), besides non-tumoral cells (MA-104) had a much lower cytotoxicity to them. Apoptotic-like cells were observed under light microscopy and were confirmed by a typical DNA fragmentation pattern. Additionally, flow cytometry results using Annexin-V/FITC permitted to quantify increased expression of early and late apoptotic markers on tumoral cells, confirming augmented Sub G0 population, which was probably associated with a consistent decrease in G1, and an increase in S or G2/M populations. Results indicate the chemical composition of V. album tinctures influences the mechanisms of in vitro tumoral cell death, suggesting a potential use in cancer pharmacotherapy research. |
topic |
Viscum album Mistletoe Lignans Phenolic compounds Antitumoral |
url |
http://www.sciencedirect.com/science/article/pii/S1319016418300112 |
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doaj-e2c21b5994a744c3b1d02f35f248eca62020-11-25T03:40:01ZengElsevierSaudi Pharmaceutical Journal1319-01642018-03-0126331132210.1016/j.jsps.2018.01.011Phenolic compounds from Viscum album tinctures enhanced antitumor activity in melanoma murine cancer cellsMichelle Nonato de Oliveira Melo0Adriana Passos Oliveira1Adalgisa Felippe Wiecikowski2Renato Sampaio Carvalho3Juliana de Lima Castro4Felipe Alves Gomes de Oliveira5Henrique Marcelo Gualberto Pereira6Venicio Feo da Veiga7Marcia Marques Alves Capella8Leandro Rocha9Carla Holandino10Multidisciplinary Laboratory of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Vegetal Biotechnology Program, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilMultidisciplinary Laboratory of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Corresponding author at: Multidisciplinary Laboratory of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio de Janeiro, Centro de Ciências da Saúde, Bloco B subsolo, sala 11, Avenida Carlos Chagas Filho 373, Ilha do Fundão/Cidade Universitária, Zip code: 21941-902 Rio de Janeiro, RJ, Brazil.Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilBrazilian Doping Control Laboratory, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilBrazilian Doping Control Laboratory, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilBrazilian Doping Control Laboratory, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilMicroscopy Sector Professor Paulo de Góes, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilMultidisciplinary Laboratory of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Fluminense Federal University, Niterói, RJ, BrazilMultidisciplinary Laboratory of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilCancer is one of the biggest problems in public health worldwide. Plants have been shown important role in anticancer research. Viscum album L. (Santalaceae), commonly known as mistletoe, is a semi-parasitic plant that grows on different host trees. In complementary medicine, extracts from European mistletoe (Viscum album L.) have been used in the treatment of cancer. The study was conducted to identify chemical composition and antitumor potential of Viscum album tinctures. Chemical analysis performed by high resolution chromatography equipped with high resolution mass spectrometer identified caffeic acid, chlorogenic acid, sakuranetin, isosakuranetin, syringenin 4-O-glucoside, syringenin 4-O-apiosyl-glucoside, alangilignoside C and ligalbumoside A compounds. Some of these compounds are probably responsible for the reduction of tumoral cellular growth in a dose-dependent manner. It was observed that melanoma murine cells (B16F10) were more sensitive to V. album tinctures than human leukaemic cells (K562), besides non-tumoral cells (MA-104) had a much lower cytotoxicity to them. Apoptotic-like cells were observed under light microscopy and were confirmed by a typical DNA fragmentation pattern. Additionally, flow cytometry results using Annexin-V/FITC permitted to quantify increased expression of early and late apoptotic markers on tumoral cells, confirming augmented Sub G0 population, which was probably associated with a consistent decrease in G1, and an increase in S or G2/M populations. Results indicate the chemical composition of V. album tinctures influences the mechanisms of in vitro tumoral cell death, suggesting a potential use in cancer pharmacotherapy research.http://www.sciencedirect.com/science/article/pii/S1319016418300112Viscum albumMistletoeLignansPhenolic compoundsAntitumoral |