Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)
The aim of this study was to evaluate cryoprotectants for cooling embryos of B. orbignyanus. 1400 embryos were selected, in the post-gastrulation and subjected to cooling to -8°C with different combinations of intracellular cryoprotectants (methanol and 9% DMSO or associated 4.5%) combined with extr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Londrina
2014-09-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/15432 |