Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.
<h4>Background</h4>The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds.<h4>Methodology</h4>The whole sequence of plasmid pGUE-NDM carrying the bla(NDM-1) gen...
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doaj-e2ca0dab02ba4dc793840608adadb6ae2021-03-04T00:52:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3475210.1371/journal.pone.0034752Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.Rémy A BonninLaurent PoirelAlessandra CarattoliPatrice Nordmann<h4>Background</h4>The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds.<h4>Methodology</h4>The whole sequence of plasmid pGUE-NDM carrying the bla(NDM-1) gene was determined by high-density pyrosequencing and a genomic comparative analysis with other bla(NDM-1)-negative IncFII was performed.<h4>Principal findings</h4>Plasmid pGUE-NDM replicating in Escherichia coli confers resistance to many antibiotic molecules including β-lactams, aminoglycosides, trimethoprim, and sulfonamides. It is 87,022 bp in-size and carries the two β-lactamase genes bla(NDM-1) and bla(OXA-1), together with three aminoglycoside resistance genes aacA4, aadA2, and aacC2. Comparative analysis of the multidrug resistance locus contained a module encompassing the bla(NDM-1) gene that is actually conserved among different structures identified in other enterobacterial isolates. This module was constituted by the bla(NDM-1) gene, a fragment of insertion sequence ISAba125 and a bleomycin resistance encoding gene.<h4>Significance</h4>This is the first characterized bla(NDM-1)-carrying IncFII-type plasmid. Such association between the bla(NDM-1) gene and an IncFII-type plasmid backbone is extremely worrisome considering that this plasmid type is known to spread efficiently, as examplified with the worldwide dissemination of bla(CTX-M-15)-borne IncFII plasmids.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22511964/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rémy A Bonnin Laurent Poirel Alessandra Carattoli Patrice Nordmann |
spellingShingle |
Rémy A Bonnin Laurent Poirel Alessandra Carattoli Patrice Nordmann Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131. PLoS ONE |
author_facet |
Rémy A Bonnin Laurent Poirel Alessandra Carattoli Patrice Nordmann |
author_sort |
Rémy A Bonnin |
title |
Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131. |
title_short |
Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131. |
title_full |
Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131. |
title_fullStr |
Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131. |
title_full_unstemmed |
Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131. |
title_sort |
characterization of an incfii plasmid encoding ndm-1 from escherichia coli st131. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
<h4>Background</h4>The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds.<h4>Methodology</h4>The whole sequence of plasmid pGUE-NDM carrying the bla(NDM-1) gene was determined by high-density pyrosequencing and a genomic comparative analysis with other bla(NDM-1)-negative IncFII was performed.<h4>Principal findings</h4>Plasmid pGUE-NDM replicating in Escherichia coli confers resistance to many antibiotic molecules including β-lactams, aminoglycosides, trimethoprim, and sulfonamides. It is 87,022 bp in-size and carries the two β-lactamase genes bla(NDM-1) and bla(OXA-1), together with three aminoglycoside resistance genes aacA4, aadA2, and aacC2. Comparative analysis of the multidrug resistance locus contained a module encompassing the bla(NDM-1) gene that is actually conserved among different structures identified in other enterobacterial isolates. This module was constituted by the bla(NDM-1) gene, a fragment of insertion sequence ISAba125 and a bleomycin resistance encoding gene.<h4>Significance</h4>This is the first characterized bla(NDM-1)-carrying IncFII-type plasmid. Such association between the bla(NDM-1) gene and an IncFII-type plasmid backbone is extremely worrisome considering that this plasmid type is known to spread efficiently, as examplified with the worldwide dissemination of bla(CTX-M-15)-borne IncFII plasmids. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22511964/pdf/?tool=EBI |
work_keys_str_mv |
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