Genotyping-by-Sequencing to Unlock Genetic Diversity and Population Structure in White Yam (<i>Dioscorea rotundata</i> Poir.)
White yam (<i>Dioscorea</i><i>rotundata</i> Poir.) is one of the most important tuber crops in West Africa, where it is indigenous and represents the largest repository of biodiversity through several years of domestication, production, consumption, and trade. In this study,...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-09-01
|
Series: | Agronomy |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4395/10/9/1437 |
id |
doaj-e2e5ca97851949d69be7cb17d329f1ea |
---|---|
record_format |
Article |
spelling |
doaj-e2e5ca97851949d69be7cb17d329f1ea2021-04-02T16:57:54ZengMDPI AGAgronomy2073-43952020-09-01101437143710.3390/agronomy10091437Genotyping-by-Sequencing to Unlock Genetic Diversity and Population Structure in White Yam (<i>Dioscorea rotundata</i> Poir.)Ranjana Bhattacharjee0Paterne Agre1Guillaume Bauchet2David De Koeyer3Antonio Lopez-Montes4P. Lava Kumar5Michael Abberton6Patrick Adebola7Asrat Asfaw8Robert Asiedu9International Institute of Tropical Agriculture, PMB 5320, Ibadan 200001, NigeriaInternational Institute of Tropical Agriculture, PMB 5320, Ibadan 200001, NigeriaBoyce Thompson Institute, Cornell University, Ithaca, NY 14853, USAAgriculture and Agri-Food Canada, Fredericton, NB E3B 4Z7, CanadaInternational Institute of Tropical Agriculture, PMB 5320, Ibadan 200001, NigeriaInternational Institute of Tropical Agriculture, PMB 5320, Ibadan 200001, NigeriaInternational Institute of Tropical Agriculture, PMB 5320, Ibadan 200001, NigeriaInternational Institute of Tropical Agriculture, PMB 82, Kubuwa, Abuja 901101, NigeriaInternational Institute of Tropical Agriculture, PMB 82, Kubuwa, Abuja 901101, NigeriaInternational Institute of Tropical Agriculture, PMB 5320, Ibadan 200001, NigeriaWhite yam (<i>Dioscorea</i><i>rotundata</i> Poir.) is one of the most important tuber crops in West Africa, where it is indigenous and represents the largest repository of biodiversity through several years of domestication, production, consumption, and trade. In this study, the genotyping-by-sequencing (GBS) approach was used to sequence 814 genotypes consisting of genebank landraces, breeding lines, and market varieties to understand the level of genetic diversity and pattern of the population structure among them. The genetic diversity among different genotypes was assessed using three complementary clustering methods, the model-based admixture, discriminant analysis of principal components (DAPC), and phylogenetic tree. ADMIXTURE analysis revealed an optimum number of four groups that matched with the number of clusters obtained through phylogenetic tree. Clustering results obtained from ADMIXTURE analysis were further validated using DAPC-based clustering. Analysis of molecular variance (AMOVA) revealed high genetic diversity (96%) within each genetic group. A network analysis was further carried out to depict the genetic relationships among the three genetic groups (breeding lines, genebank landraces, and market varieties) used in the study. This study showed that the use of advanced sequencing techniques such as GBS coupled with statistical analysis is a robust method for assessing genetic diversity and population structure in a complex crop such as white yam.https://www.mdpi.com/2073-4395/10/9/1437white yamWest AfricaSNP markersgenetic diversitypopulation structure |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ranjana Bhattacharjee Paterne Agre Guillaume Bauchet David De Koeyer Antonio Lopez-Montes P. Lava Kumar Michael Abberton Patrick Adebola Asrat Asfaw Robert Asiedu |
spellingShingle |
Ranjana Bhattacharjee Paterne Agre Guillaume Bauchet David De Koeyer Antonio Lopez-Montes P. Lava Kumar Michael Abberton Patrick Adebola Asrat Asfaw Robert Asiedu Genotyping-by-Sequencing to Unlock Genetic Diversity and Population Structure in White Yam (<i>Dioscorea rotundata</i> Poir.) Agronomy white yam West Africa SNP markers genetic diversity population structure |
author_facet |
Ranjana Bhattacharjee Paterne Agre Guillaume Bauchet David De Koeyer Antonio Lopez-Montes P. Lava Kumar Michael Abberton Patrick Adebola Asrat Asfaw Robert Asiedu |
author_sort |
Ranjana Bhattacharjee |
title |
Genotyping-by-Sequencing to Unlock Genetic Diversity and Population Structure in White Yam (<i>Dioscorea rotundata</i> Poir.) |
title_short |
Genotyping-by-Sequencing to Unlock Genetic Diversity and Population Structure in White Yam (<i>Dioscorea rotundata</i> Poir.) |
title_full |
Genotyping-by-Sequencing to Unlock Genetic Diversity and Population Structure in White Yam (<i>Dioscorea rotundata</i> Poir.) |
title_fullStr |
Genotyping-by-Sequencing to Unlock Genetic Diversity and Population Structure in White Yam (<i>Dioscorea rotundata</i> Poir.) |
title_full_unstemmed |
Genotyping-by-Sequencing to Unlock Genetic Diversity and Population Structure in White Yam (<i>Dioscorea rotundata</i> Poir.) |
title_sort |
genotyping-by-sequencing to unlock genetic diversity and population structure in white yam (<i>dioscorea rotundata</i> poir.) |
publisher |
MDPI AG |
series |
Agronomy |
issn |
2073-4395 |
publishDate |
2020-09-01 |
description |
White yam (<i>Dioscorea</i><i>rotundata</i> Poir.) is one of the most important tuber crops in West Africa, where it is indigenous and represents the largest repository of biodiversity through several years of domestication, production, consumption, and trade. In this study, the genotyping-by-sequencing (GBS) approach was used to sequence 814 genotypes consisting of genebank landraces, breeding lines, and market varieties to understand the level of genetic diversity and pattern of the population structure among them. The genetic diversity among different genotypes was assessed using three complementary clustering methods, the model-based admixture, discriminant analysis of principal components (DAPC), and phylogenetic tree. ADMIXTURE analysis revealed an optimum number of four groups that matched with the number of clusters obtained through phylogenetic tree. Clustering results obtained from ADMIXTURE analysis were further validated using DAPC-based clustering. Analysis of molecular variance (AMOVA) revealed high genetic diversity (96%) within each genetic group. A network analysis was further carried out to depict the genetic relationships among the three genetic groups (breeding lines, genebank landraces, and market varieties) used in the study. This study showed that the use of advanced sequencing techniques such as GBS coupled with statistical analysis is a robust method for assessing genetic diversity and population structure in a complex crop such as white yam. |
topic |
white yam West Africa SNP markers genetic diversity population structure |
url |
https://www.mdpi.com/2073-4395/10/9/1437 |
work_keys_str_mv |
AT ranjanabhattacharjee genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir AT paterneagre genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir AT guillaumebauchet genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir AT daviddekoeyer genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir AT antoniolopezmontes genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir AT plavakumar genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir AT michaelabberton genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir AT patrickadebola genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir AT asratasfaw genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir AT robertasiedu genotypingbysequencingtounlockgeneticdiversityandpopulationstructureinwhiteyamidioscorearotundataipoir |
_version_ |
1721554980284923904 |