Bioluminescence imaging to track bacterial dissemination of <it>Yersinia pestis</it> using different routes of infection in mice
<p>Abstract</p> <p>Background</p> <p>Plague is caused by <it>Yersinia pestis</it>, a bacterium that disseminates inside of the host at remarkably high rates. Plague bacilli disrupt normal immune responses in the host allowing for systematic spread that is fa...
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doaj-e2efb756a9ff4039b969c7801e50018b2020-11-25T00:15:13ZengBMCBMC Microbiology1471-21802012-07-0112114710.1186/1471-2180-12-147Bioluminescence imaging to track bacterial dissemination of <it>Yersinia pestis</it> using different routes of infection in miceGonzalez Rodrigo JWeening Eric HFrothingham RichardSempowski Gregory DMiller Virginia L<p>Abstract</p> <p>Background</p> <p>Plague is caused by <it>Yersinia pestis</it>, a bacterium that disseminates inside of the host at remarkably high rates. Plague bacilli disrupt normal immune responses in the host allowing for systematic spread that is fatal if left untreated. How <it>Y. pestis</it> disseminates from the site of infection to deeper tissues is unknown. Dissemination studies for plague are typically performed in mice by determining the bacterial burden in specific organs at various time points. To follow bacterial dissemination during plague infections in mice we tested the possibility of using bioluminescence imaging (BLI), an alternative non-invasive approach. Fully virulent <it>Y. pestis</it> was transformed with a plasmid containing the <it>luxCDABE</it> genes, making it able to produce light; this <it>lux</it>-expressing strain was used to infect mice by subcutaneous, intradermal or intranasal inoculation.</p> <p>Results</p> <p>We successfully obtained images from infected animals and were able to follow bacterial dissemination over time for each of the three different routes of inoculation. We also compared the radiance signal from animals infected with a wild type strain and a Δ<it>caf1</it>Δ<it>psaA</it> mutant that we previously showed to be attenuated in colonization of the lymph node and systemic dissemination. Radiance signals from mice infected with the wild type strain were larger than values obtained from mice infected with the mutant strain (linear regression of normalized values, P < 0.05).</p> <p>Conclusions</p> <p>We demonstrate that BLI is useful for monitoring dissemination from multiple inoculation sites, and for characterization of mutants with defects in colonization or dissemination.</p> http://www.biomedcentral.com/1471-2180/12/147PlagueBioluminescenceIn vivo imagingBacterial dissemination |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gonzalez Rodrigo J Weening Eric H Frothingham Richard Sempowski Gregory D Miller Virginia L |
spellingShingle |
Gonzalez Rodrigo J Weening Eric H Frothingham Richard Sempowski Gregory D Miller Virginia L Bioluminescence imaging to track bacterial dissemination of <it>Yersinia pestis</it> using different routes of infection in mice BMC Microbiology Plague Bioluminescence In vivo imaging Bacterial dissemination |
author_facet |
Gonzalez Rodrigo J Weening Eric H Frothingham Richard Sempowski Gregory D Miller Virginia L |
author_sort |
Gonzalez Rodrigo J |
title |
Bioluminescence imaging to track bacterial dissemination of <it>Yersinia pestis</it> using different routes of infection in mice |
title_short |
Bioluminescence imaging to track bacterial dissemination of <it>Yersinia pestis</it> using different routes of infection in mice |
title_full |
Bioluminescence imaging to track bacterial dissemination of <it>Yersinia pestis</it> using different routes of infection in mice |
title_fullStr |
Bioluminescence imaging to track bacterial dissemination of <it>Yersinia pestis</it> using different routes of infection in mice |
title_full_unstemmed |
Bioluminescence imaging to track bacterial dissemination of <it>Yersinia pestis</it> using different routes of infection in mice |
title_sort |
bioluminescence imaging to track bacterial dissemination of <it>yersinia pestis</it> using different routes of infection in mice |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2012-07-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Plague is caused by <it>Yersinia pestis</it>, a bacterium that disseminates inside of the host at remarkably high rates. Plague bacilli disrupt normal immune responses in the host allowing for systematic spread that is fatal if left untreated. How <it>Y. pestis</it> disseminates from the site of infection to deeper tissues is unknown. Dissemination studies for plague are typically performed in mice by determining the bacterial burden in specific organs at various time points. To follow bacterial dissemination during plague infections in mice we tested the possibility of using bioluminescence imaging (BLI), an alternative non-invasive approach. Fully virulent <it>Y. pestis</it> was transformed with a plasmid containing the <it>luxCDABE</it> genes, making it able to produce light; this <it>lux</it>-expressing strain was used to infect mice by subcutaneous, intradermal or intranasal inoculation.</p> <p>Results</p> <p>We successfully obtained images from infected animals and were able to follow bacterial dissemination over time for each of the three different routes of inoculation. We also compared the radiance signal from animals infected with a wild type strain and a Δ<it>caf1</it>Δ<it>psaA</it> mutant that we previously showed to be attenuated in colonization of the lymph node and systemic dissemination. Radiance signals from mice infected with the wild type strain were larger than values obtained from mice infected with the mutant strain (linear regression of normalized values, P < 0.05).</p> <p>Conclusions</p> <p>We demonstrate that BLI is useful for monitoring dissemination from multiple inoculation sites, and for characterization of mutants with defects in colonization or dissemination.</p> |
topic |
Plague Bioluminescence In vivo imaging Bacterial dissemination |
url |
http://www.biomedcentral.com/1471-2180/12/147 |
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