Differential effects of vagus nerve stimulation strategies on glycemia and pancreatic secretions
Abstract Despite advancements in pharmacotherapies, glycemia is poorly controlled in type 2 diabetic patients. As the vagus nerve regulates energy metabolism, here we evaluated the effect various electrical vagus nerve stimulation strategies have on glycemia and glucose‐regulating hormones, as a fir...
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doaj-e2f1d23ad4de481db36f00b6bf1aeeb82020-11-25T03:50:09ZengWileyPhysiological Reports2051-817X2020-06-01811n/an/a10.14814/phy2.14479Differential effects of vagus nerve stimulation strategies on glycemia and pancreatic secretionsSophie C. Payne0Glenn Ward1Richard J. MacIsaac2Tomoko Hyakumura3James B. Fallon4Joel Villalobos5Bionics Institute East Melbourne Vic. AustraliaBionics Institute East Melbourne Vic. AustraliaBionics Institute East Melbourne Vic. AustraliaBionics Institute East Melbourne Vic. AustraliaBionics Institute East Melbourne Vic. AustraliaBionics Institute East Melbourne Vic. AustraliaAbstract Despite advancements in pharmacotherapies, glycemia is poorly controlled in type 2 diabetic patients. As the vagus nerve regulates energy metabolism, here we evaluated the effect various electrical vagus nerve stimulation strategies have on glycemia and glucose‐regulating hormones, as a first step to developing a novel therapy of type 2 diabetes. Sprague–Dawley rats were anesthetized, the abdominal (anterior) vagus nerve implanted, and various stimulation strategies applied to the nerve: (a) 15 Hz; (b) 4 kHz, or 40 kHz and; (c) a combination of 15 Hz and 40 kHz to directionally activate afferent or efferent vagal fibers. Following a glucose bolus (500 mg/kg, I.V.), stimulation strategies were applied (60 min) and serial blood samples taken. No stimulation was used as a crossover control sequence. Applying 15 Hz stimulation significantly increased glucose (+2.9 ± 0.2 mM·hr, p = .015) and glucagon (+17.1 ± 8.0 pg·hr/ml, p = .022), compared to no stimulation. Application of 4 kHz stimulation also significantly increased glucose levels (+1.5 ± 0.5 mM·hr, p = .049), while 40 kHz frequency stimulation resulted in no changes to glucose levels but did significantly lower glucagon (−12.3 ± 1.1 pg·hr/ml, p = .0009). Directional afferent stimulation increased glucose (+2.4 ± 1.5 mM·hr) and glucagon levels (+39.5 ± 15.0 pg·hr/ml). Despite hyperglycemia resulting when VNS, aVNS, and 4 kHz stimulation strategies were applied, the changes in insulin levels were not significant (p ≥ .05). In summary, vagus nerve stimulation modulates glycemia by effecting glucagon and insulin secretions, and high‐frequency 40 kHz stimulation may have potential application for the treatment of type 2 diabetes.https://doi.org/10.14814/phy2.14479bioelectronic medicinemedical devicesperipheral nerve stimulationtype 2 diabetes mellitus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sophie C. Payne Glenn Ward Richard J. MacIsaac Tomoko Hyakumura James B. Fallon Joel Villalobos |
spellingShingle |
Sophie C. Payne Glenn Ward Richard J. MacIsaac Tomoko Hyakumura James B. Fallon Joel Villalobos Differential effects of vagus nerve stimulation strategies on glycemia and pancreatic secretions Physiological Reports bioelectronic medicine medical devices peripheral nerve stimulation type 2 diabetes mellitus |
author_facet |
Sophie C. Payne Glenn Ward Richard J. MacIsaac Tomoko Hyakumura James B. Fallon Joel Villalobos |
author_sort |
Sophie C. Payne |
title |
Differential effects of vagus nerve stimulation strategies on glycemia and pancreatic secretions |
title_short |
Differential effects of vagus nerve stimulation strategies on glycemia and pancreatic secretions |
title_full |
Differential effects of vagus nerve stimulation strategies on glycemia and pancreatic secretions |
title_fullStr |
Differential effects of vagus nerve stimulation strategies on glycemia and pancreatic secretions |
title_full_unstemmed |
Differential effects of vagus nerve stimulation strategies on glycemia and pancreatic secretions |
title_sort |
differential effects of vagus nerve stimulation strategies on glycemia and pancreatic secretions |
publisher |
Wiley |
series |
Physiological Reports |
issn |
2051-817X |
publishDate |
2020-06-01 |
description |
Abstract Despite advancements in pharmacotherapies, glycemia is poorly controlled in type 2 diabetic patients. As the vagus nerve regulates energy metabolism, here we evaluated the effect various electrical vagus nerve stimulation strategies have on glycemia and glucose‐regulating hormones, as a first step to developing a novel therapy of type 2 diabetes. Sprague–Dawley rats were anesthetized, the abdominal (anterior) vagus nerve implanted, and various stimulation strategies applied to the nerve: (a) 15 Hz; (b) 4 kHz, or 40 kHz and; (c) a combination of 15 Hz and 40 kHz to directionally activate afferent or efferent vagal fibers. Following a glucose bolus (500 mg/kg, I.V.), stimulation strategies were applied (60 min) and serial blood samples taken. No stimulation was used as a crossover control sequence. Applying 15 Hz stimulation significantly increased glucose (+2.9 ± 0.2 mM·hr, p = .015) and glucagon (+17.1 ± 8.0 pg·hr/ml, p = .022), compared to no stimulation. Application of 4 kHz stimulation also significantly increased glucose levels (+1.5 ± 0.5 mM·hr, p = .049), while 40 kHz frequency stimulation resulted in no changes to glucose levels but did significantly lower glucagon (−12.3 ± 1.1 pg·hr/ml, p = .0009). Directional afferent stimulation increased glucose (+2.4 ± 1.5 mM·hr) and glucagon levels (+39.5 ± 15.0 pg·hr/ml). Despite hyperglycemia resulting when VNS, aVNS, and 4 kHz stimulation strategies were applied, the changes in insulin levels were not significant (p ≥ .05). In summary, vagus nerve stimulation modulates glycemia by effecting glucagon and insulin secretions, and high‐frequency 40 kHz stimulation may have potential application for the treatment of type 2 diabetes. |
topic |
bioelectronic medicine medical devices peripheral nerve stimulation type 2 diabetes mellitus |
url |
https://doi.org/10.14814/phy2.14479 |
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