Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency
Peptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (PNA) invasion does not require the prior dena...
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Online Access: | https://www.mdpi.com/2076-3417/10/23/8663 |
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doaj-e2faafab27444270b2fd77a0bd6dd4ed2020-12-04T00:05:07ZengMDPI AGApplied Sciences2076-34172020-12-01108663866310.3390/app10238663Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion EfficiencyYuichiro Aiba0Gerardo Urbina1Masanari Shibata2Osami Shoji3Department of Chemistry, Graduate School of Science, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Aichi 464-8602, JapanDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Aichi 464-8602, JapanDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Aichi 464-8602, JapanDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Aichi 464-8602, JapanPeptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (PNA) invasion does not require the prior denaturation of dsDNA. To increase its applicability, we developed PNAs modified with a nuclear localisation signal (NLS) peptide. In this study, the characteristics of NLS-modified PNAs were investigated for the future design of novel peptide-modified PNAs.https://www.mdpi.com/2076-3417/10/23/8663PNAinvasionDNANLS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuichiro Aiba Gerardo Urbina Masanari Shibata Osami Shoji |
spellingShingle |
Yuichiro Aiba Gerardo Urbina Masanari Shibata Osami Shoji Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency Applied Sciences PNA invasion DNA NLS |
author_facet |
Yuichiro Aiba Gerardo Urbina Masanari Shibata Osami Shoji |
author_sort |
Yuichiro Aiba |
title |
Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency |
title_short |
Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency |
title_full |
Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency |
title_fullStr |
Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency |
title_full_unstemmed |
Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency |
title_sort |
investigation of the characteristics of nls-pna: influence of nls location on invasion efficiency |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2020-12-01 |
description |
Peptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (PNA) invasion does not require the prior denaturation of dsDNA. To increase its applicability, we developed PNAs modified with a nuclear localisation signal (NLS) peptide. In this study, the characteristics of NLS-modified PNAs were investigated for the future design of novel peptide-modified PNAs. |
topic |
PNA invasion DNA NLS |
url |
https://www.mdpi.com/2076-3417/10/23/8663 |
work_keys_str_mv |
AT yuichiroaiba investigationofthecharacteristicsofnlspnainfluenceofnlslocationoninvasionefficiency AT gerardourbina investigationofthecharacteristicsofnlspnainfluenceofnlslocationoninvasionefficiency AT masanarishibata investigationofthecharacteristicsofnlspnainfluenceofnlslocationoninvasionefficiency AT osamishoji investigationofthecharacteristicsofnlspnainfluenceofnlslocationoninvasionefficiency |
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