Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency

Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency

Peptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (PNA) invasion does not require the prior dena...

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Bibliographic Details
Main Authors: Yuichiro Aiba, Gerardo Urbina, Masanari Shibata, Osami Shoji
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Applied Sciences
Subjects:
PNA
invasion
DNA
NLS
Online Access:https://www.mdpi.com/2076-3417/10/23/8663
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spelling doaj-e2faafab27444270b2fd77a0bd6dd4ed2020-12-04T00:05:07ZengMDPI AGApplied Sciences2076-34172020-12-01108663866310.3390/app10238663Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion EfficiencyYuichiro Aiba0Gerardo Urbina1Masanari Shibata2Osami Shoji3Department of Chemistry, Graduate School of Science, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Aichi 464-8602, JapanDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Aichi 464-8602, JapanDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Aichi 464-8602, JapanDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Aichi 464-8602, JapanPeptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (PNA) invasion does not require the prior denaturation of dsDNA. To increase its applicability, we developed PNAs modified with a nuclear localisation signal (NLS) peptide. In this study, the characteristics of NLS-modified PNAs were investigated for the future design of novel peptide-modified PNAs.https://www.mdpi.com/2076-3417/10/23/8663PNAinvasionDNANLS
collection DOAJ
language English
format Article
sources DOAJ
author Yuichiro Aiba
Gerardo Urbina
Masanari Shibata
Osami Shoji
spellingShingle Yuichiro Aiba
Gerardo Urbina
Masanari Shibata
Osami Shoji
Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency
Applied Sciences
PNA
invasion
DNA
NLS
author_facet Yuichiro Aiba
Gerardo Urbina
Masanari Shibata
Osami Shoji
author_sort Yuichiro Aiba
title Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency
title_short Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency
title_full Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency
title_fullStr Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency
title_full_unstemmed Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency
title_sort investigation of the characteristics of nls-pna: influence of nls location on invasion efficiency
publisher MDPI AG
series Applied Sciences
issn 2076-3417
publishDate 2020-12-01
description Peptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (PNA) invasion does not require the prior denaturation of dsDNA. To increase its applicability, we developed PNAs modified with a nuclear localisation signal (NLS) peptide. In this study, the characteristics of NLS-modified PNAs were investigated for the future design of novel peptide-modified PNAs.
topic PNA
invasion
DNA
NLS
url https://www.mdpi.com/2076-3417/10/23/8663
work_keys_str_mv AT yuichiroaiba investigationofthecharacteristicsofnlspnainfluenceofnlslocationoninvasionefficiency
AT gerardourbina investigationofthecharacteristicsofnlspnainfluenceofnlslocationoninvasionefficiency
AT masanarishibata investigationofthecharacteristicsofnlspnainfluenceofnlslocationoninvasionefficiency
AT osamishoji investigationofthecharacteristicsofnlspnainfluenceofnlslocationoninvasionefficiency
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