Alteration of Phenolic Composition in Lettuce (<i>Lactuca sativa</i> L.) by Reducing Nitrogen Supply Enhances its Anti-Proliferative Effects on Colorectal Cancer Cells
Consumption of vegetables rich in phenolic compounds has become a useful method to reduce the risk of developing several types of cancer. This study investigated the potential relationship between the alteration of phenolic compounds in lettuce induced by reduced nitrogen supply and its anti-prolife...
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doaj-e3183fcee6574250bd81c537474238f02020-11-25T02:47:44ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-08-012017420510.3390/ijms20174205ijms20174205Alteration of Phenolic Composition in Lettuce (<i>Lactuca sativa</i> L.) by Reducing Nitrogen Supply Enhances its Anti-Proliferative Effects on Colorectal Cancer CellsWeiwei Zhou0Xin Liang1Peibin Dai2Yao Chen3Yuxue Zhang4Miao Zhang5Lingli Lu6Chongwei Jin7Xianyong Lin8MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, ChinaMOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Applied Engineering, Zhejiang Economic and Trade Polytechnic, Hangzhou 310018, ChinaMOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, ChinaMOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, ChinaMOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, ChinaMOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, ChinaMOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, ChinaMOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, ChinaConsumption of vegetables rich in phenolic compounds has become a useful method to reduce the risk of developing several types of cancer. This study investigated the potential relationship between the alteration of phenolic compounds in lettuce induced by reduced nitrogen supply and its anti-proliferative effects on Caco-2 colorectal cancer cells. Our results showed that phenolic extracts from lettuce grown under low nitrogen conditions (LP) exhibited better anti-proliferative effects against Caco-2 cells, in part, by interfering with the cell cycle and inducing apoptosis, compared with those from lettuce supplied with adequate nitrogen. High performance liquid chromatography (HPLC) analysis and correlation analysis indicated that the better anticancer activity of LP may be not only related to the increased phenolic content, but also associated with the increased percentage contribution of quercetin to total phenolics. Taken together, alteration of phenolic composition by reduced nitrogen supply can be an effectively strategy for the development of healthy vegetables as anticancer products.https://www.mdpi.com/1422-0067/20/17/4205nitrogenphenolic compoundsanti-proliferationapoptosiscolorectal cancer<i>Lactuca sativa</i> L. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Weiwei Zhou Xin Liang Peibin Dai Yao Chen Yuxue Zhang Miao Zhang Lingli Lu Chongwei Jin Xianyong Lin |
spellingShingle |
Weiwei Zhou Xin Liang Peibin Dai Yao Chen Yuxue Zhang Miao Zhang Lingli Lu Chongwei Jin Xianyong Lin Alteration of Phenolic Composition in Lettuce (<i>Lactuca sativa</i> L.) by Reducing Nitrogen Supply Enhances its Anti-Proliferative Effects on Colorectal Cancer Cells International Journal of Molecular Sciences nitrogen phenolic compounds anti-proliferation apoptosis colorectal cancer <i>Lactuca sativa</i> L. |
author_facet |
Weiwei Zhou Xin Liang Peibin Dai Yao Chen Yuxue Zhang Miao Zhang Lingli Lu Chongwei Jin Xianyong Lin |
author_sort |
Weiwei Zhou |
title |
Alteration of Phenolic Composition in Lettuce (<i>Lactuca sativa</i> L.) by Reducing Nitrogen Supply Enhances its Anti-Proliferative Effects on Colorectal Cancer Cells |
title_short |
Alteration of Phenolic Composition in Lettuce (<i>Lactuca sativa</i> L.) by Reducing Nitrogen Supply Enhances its Anti-Proliferative Effects on Colorectal Cancer Cells |
title_full |
Alteration of Phenolic Composition in Lettuce (<i>Lactuca sativa</i> L.) by Reducing Nitrogen Supply Enhances its Anti-Proliferative Effects on Colorectal Cancer Cells |
title_fullStr |
Alteration of Phenolic Composition in Lettuce (<i>Lactuca sativa</i> L.) by Reducing Nitrogen Supply Enhances its Anti-Proliferative Effects on Colorectal Cancer Cells |
title_full_unstemmed |
Alteration of Phenolic Composition in Lettuce (<i>Lactuca sativa</i> L.) by Reducing Nitrogen Supply Enhances its Anti-Proliferative Effects on Colorectal Cancer Cells |
title_sort |
alteration of phenolic composition in lettuce (<i>lactuca sativa</i> l.) by reducing nitrogen supply enhances its anti-proliferative effects on colorectal cancer cells |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-08-01 |
description |
Consumption of vegetables rich in phenolic compounds has become a useful method to reduce the risk of developing several types of cancer. This study investigated the potential relationship between the alteration of phenolic compounds in lettuce induced by reduced nitrogen supply and its anti-proliferative effects on Caco-2 colorectal cancer cells. Our results showed that phenolic extracts from lettuce grown under low nitrogen conditions (LP) exhibited better anti-proliferative effects against Caco-2 cells, in part, by interfering with the cell cycle and inducing apoptosis, compared with those from lettuce supplied with adequate nitrogen. High performance liquid chromatography (HPLC) analysis and correlation analysis indicated that the better anticancer activity of LP may be not only related to the increased phenolic content, but also associated with the increased percentage contribution of quercetin to total phenolics. Taken together, alteration of phenolic composition by reduced nitrogen supply can be an effectively strategy for the development of healthy vegetables as anticancer products. |
topic |
nitrogen phenolic compounds anti-proliferation apoptosis colorectal cancer <i>Lactuca sativa</i> L. |
url |
https://www.mdpi.com/1422-0067/20/17/4205 |
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