Toward an accessible and robust in vitro approach to evaluate bacterial viability in the upper gastro-intestinal tract: A Gastro-Intestinal Digestive Simulator (GIDS) combined with alternative methods to plating

Toward an accessible and robust in vitro approach to evaluate bacterial viability in the upper gastro-intestinal tract: A Gastro-Intestinal Digestive Simulator (GIDS) combined with alternative methods to plating

Since the viability throughout the human gastro-intestinal (GI) tract is a key metric to select new probiotics, we aimed to develop an evolutive and robust semi-dynamic model, named Gastro-Intestinal Digestive Simulator (GIDS), mimicking pH variations of the upper GI tract and based on the latest In...

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Bibliographic Details
Main Authors: Nadège Adouard, Junji Fujimoto, Kaihei Oki, Takuya Akiyama, Yasmina Belaïnoussi, Christèle Tison, Lolita Maufoux, Rémi Brazeilles, Biliana Lesic, Raphaël Moriez, Kenji Oishi, Sophie Legrain-Raspaud
Format: Article
Language:English
Published: Elsevier 2019-08-01
Series:Journal of Functional Foods
Subjects:
Gastro-intestinal in vitro model
Viability
Bacteria
PMA-qPCR
Flow cytometry
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464619302828

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