Effect of Toxic Components on Microbial Fuel Cell-Polarization Curves and Estimation of the Type of Toxic Inhibition

• Main Authors: Karel J. Keesman, Gerrit van Straten, Hubertus V. M. Hamelers, Nienke E. Stein
• Format: Article
• Language: English
• Published: MDPI AG 2012-07-01
• Series: Biosensors
• Subjects: toxicity detection; microbial fuel cell; biosensor; least square estimation; linear regression
• Online Access: http://www.mdpi.com/2079-6374/2/3/255

Polarization curves are of paramount importance for the detection of toxic components in microbial fuel cell (MFC) based biosensors. In this study, polarization curves were made under non-toxic conditions and under toxic conditions after the addition of various concentrations of nickel, bentazon, sodiumdodecyl sulfate and potassium ferricyanide. The experimental polarization curves show that toxic components have an effect on the electrochemically active bacteria in the cell. (Extended) Butler Volmer Monod (BVM) models were used to describe the polarization curves of the MFC under nontoxic and toxic conditions. It was possible to properly fit the (extended) BVM models using linear regression techniques to the polarization curves and to distinguish between different types of kinetic inhibitions. For each of the toxic components, the value of the kinetic inhibition constant Ki was also estimated from the experimental data. The value of Ki indicates the sensitivity of the sensor for a specific component and thus can be used for the selection of the biosensor for a toxic component.