In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline

• Main Authors: Samaneh Ghasemi, Kamran Sardari, Pezhman Mirshokraei, Hossein Hassanpour
• Format: Article
• Language: English
• Published: Iranian Veterinary Surgery Association (IVSA) 2017-10-01
• Series: Iranian Journal of Veterinary Surgery
• Subjects: Doxycycline; Equine fibroblast-like synoviocytes; MTT assay; Trypn blue; viability
• Online Access: http://www.ivsajournals.com/article_50823_2dead6e226d813e8adf97f64c3e73a49.pdf

Objective- The purpose of present study was to investigate the viability of equine fibroblast-like synoviocytes (FLSs) treated with doxycycline.<br /> Design- Experimental study.<br /> Sample population- FLSs from metacarpophalangeal joints of six skeletally mature horses.<br /> Methods- FLSs were established from synovial fluids of healthy joints. The cells were treated with different concentrations (1, 5, 10, 50, 100, 150, 300, 400 µg/ml) or without doxycycline for 48-hour. Viability of FLSs was determined by MTT assay and the Trypan blue dye exclusion method.<br /> Results- No significant differences were observed between viability of FLSs cultures treated with doxycycline until 150 µg/ml and control group (P>0.05). Doxycycline at 300 and 400 µg/ml significantly decreased FLSs viability (P<0.05). FLSs viability were 74.28% and 59.07% in 300 and 400 µg/ml, respectively, when measured by the MTT assay. Also FLSs viability at 300 and 400 µg/ml of doxycycline were 68.10% and 43.26%, respectively with Trypan blue exclusion method.<br /> Conclusion and Clinical Relevance- These findings demonstrate that doxycycline was not toxic for equine FLSs at concentration equal or less than 150 µg/ml in vitro. Further studies are needed to investigate the safety, efficacy and detrimental effects of doxycycline in equine joints.