Identification of key genes and pathways of diagnosis and prognosis in cervical cancer by bioinformatics analysis
Abstract Background Cervical cancer as one of the most common malignant tumors lead to bad prognosis among women. Some researches already focus on the carcinogenesis and pathogenesis of cervical cancer, but it is still necessary to identify more key genes and pathways. Methods Differentially express...
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doaj-e35af8587a8e4bd6b46b325d35325a0e2020-11-25T03:18:47ZengWileyMolecular Genetics & Genomic Medicine2324-92692020-06-0186n/an/a10.1002/mgg3.1200Identification of key genes and pathways of diagnosis and prognosis in cervical cancer by bioinformatics analysisHua‐ju Yang0Jin‐min Xue1Jie Li2Ling‐hong Wan3Yu‐xi Zhu4Department of Oncology The First Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Oncology The First Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Oncology The First Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Oncology The First Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Oncology The First Affiliated Hospital of Chongqing Medical University Chongqing ChinaAbstract Background Cervical cancer as one of the most common malignant tumors lead to bad prognosis among women. Some researches already focus on the carcinogenesis and pathogenesis of cervical cancer, but it is still necessary to identify more key genes and pathways. Methods Differentially expressed genes were identified by GEO2R from the gene expression omnibus (GEO) website, then gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyzed by DAVID. Meanwhile, protein–protein interaction network was constructed by STRING, and both key genes and modules were found in visualizing network through Cytoscape. Besides, GEPIA did the differential expression of key genes and survival analysis. Finally, the expression of genes related to prognosis was further explored by UNLCAN, oncomine, and the human protein atlas. Results Totally 57 differentially expressed genes were founded, not only enriched in G1/S transition of mitotic cell cycle, mitotic nuclear division, and cell division but also participated in cytokine–cytokine receptor interaction, toll‐like receptor signaling pathway, and amoebiasis. Additionally, 12 hub genes and 3 key modules were screened in the Cytoscape visualization network. Further survival analysis showed that TYMS (OMIM accession number 188350), MCM2 (OMIM accession number 116945), HELLS (OMIM accession number 603946), TOP2A (OMIM accession number 126430), and CXCL8 (OMIM accession number 146930) were associated with the prognosis of cervical cancer. Conclusion This study aim to better understand the characteristics of some genes and signaling pathways about cervical cancer by bioinformatics, and could provide further research ideas to find new mechanism, more prognostic factors, and potential therapeutic targets for cervical cancer.https://doi.org/10.1002/mgg3.1200bioinformatics analysiscervical cancerdiagnosis and prognosisdifferentially expressed genes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hua‐ju Yang Jin‐min Xue Jie Li Ling‐hong Wan Yu‐xi Zhu |
spellingShingle |
Hua‐ju Yang Jin‐min Xue Jie Li Ling‐hong Wan Yu‐xi Zhu Identification of key genes and pathways of diagnosis and prognosis in cervical cancer by bioinformatics analysis Molecular Genetics & Genomic Medicine bioinformatics analysis cervical cancer diagnosis and prognosis differentially expressed genes |
author_facet |
Hua‐ju Yang Jin‐min Xue Jie Li Ling‐hong Wan Yu‐xi Zhu |
author_sort |
Hua‐ju Yang |
title |
Identification of key genes and pathways of diagnosis and prognosis in cervical cancer by bioinformatics analysis |
title_short |
Identification of key genes and pathways of diagnosis and prognosis in cervical cancer by bioinformatics analysis |
title_full |
Identification of key genes and pathways of diagnosis and prognosis in cervical cancer by bioinformatics analysis |
title_fullStr |
Identification of key genes and pathways of diagnosis and prognosis in cervical cancer by bioinformatics analysis |
title_full_unstemmed |
Identification of key genes and pathways of diagnosis and prognosis in cervical cancer by bioinformatics analysis |
title_sort |
identification of key genes and pathways of diagnosis and prognosis in cervical cancer by bioinformatics analysis |
publisher |
Wiley |
series |
Molecular Genetics & Genomic Medicine |
issn |
2324-9269 |
publishDate |
2020-06-01 |
description |
Abstract Background Cervical cancer as one of the most common malignant tumors lead to bad prognosis among women. Some researches already focus on the carcinogenesis and pathogenesis of cervical cancer, but it is still necessary to identify more key genes and pathways. Methods Differentially expressed genes were identified by GEO2R from the gene expression omnibus (GEO) website, then gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyzed by DAVID. Meanwhile, protein–protein interaction network was constructed by STRING, and both key genes and modules were found in visualizing network through Cytoscape. Besides, GEPIA did the differential expression of key genes and survival analysis. Finally, the expression of genes related to prognosis was further explored by UNLCAN, oncomine, and the human protein atlas. Results Totally 57 differentially expressed genes were founded, not only enriched in G1/S transition of mitotic cell cycle, mitotic nuclear division, and cell division but also participated in cytokine–cytokine receptor interaction, toll‐like receptor signaling pathway, and amoebiasis. Additionally, 12 hub genes and 3 key modules were screened in the Cytoscape visualization network. Further survival analysis showed that TYMS (OMIM accession number 188350), MCM2 (OMIM accession number 116945), HELLS (OMIM accession number 603946), TOP2A (OMIM accession number 126430), and CXCL8 (OMIM accession number 146930) were associated with the prognosis of cervical cancer. Conclusion This study aim to better understand the characteristics of some genes and signaling pathways about cervical cancer by bioinformatics, and could provide further research ideas to find new mechanism, more prognostic factors, and potential therapeutic targets for cervical cancer. |
topic |
bioinformatics analysis cervical cancer diagnosis and prognosis differentially expressed genes |
url |
https://doi.org/10.1002/mgg3.1200 |
work_keys_str_mv |
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