Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes

Despite high relevance of tear osmolarity and eye abnormality, numerous methods for detecting tear osmolarity rely upon expensive osmometers. We report a reliable method for simply determining sodium ion-based osmolarity in artificial tears using sequential DNAzymes. When sodium ion-specific DNAzyme...

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Main Authors:	Eun Hye Kim, Eun-Song Lee, Dong Yun Lee, Young-Pil Kim
Format:	Article
Language:	English
Published:	MDPI AG 2017-12-01
Series:	Sensors
Subjects:	DNAzyme osmolarity sodium ion salinity tear dry eye
Online Access:	https://www.mdpi.com/1424-8220/17/12/2840

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spelling	doaj-e36d6391c91a4d8289db72b18511a5fc2020-11-24T20:46:28ZengMDPI AGSensors1424-82202017-12-011712284010.3390/s17122840s17122840Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymesEun Hye Kim0Eun-Song Lee1Dong Yun Lee2Young-Pil Kim3Department of Life Science, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, Hanyang University, Seoul 04763, KoreaDepartment of Bioengineering, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, Hanyang University, Seoul 04763, KoreaDespite high relevance of tear osmolarity and eye abnormality, numerous methods for detecting tear osmolarity rely upon expensive osmometers. We report a reliable method for simply determining sodium ion-based osmolarity in artificial tears using sequential DNAzymes. When sodium ion-specific DNAzyme and peroxidase-like DNAzyme were used as a sensing and detecting probe, respectively, the concentration of Na+ in artificial tears could be measured by absorbance or fluorescence intensity, which was highly correlated with osmolarity over the diagnostic range (R2 > 0.98). Our approach is useful for studying eye diseases in relation to osmolarity.https://www.mdpi.com/1424-8220/17/12/2840DNAzymeosmolaritysodium ionsalinityteardry eye
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Eun Hye Kim Eun-Song Lee Dong Yun Lee Young-Pil Kim
spellingShingle	Eun Hye Kim Eun-Song Lee Dong Yun Lee Young-Pil Kim Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes Sensors DNAzyme osmolarity sodium ion salinity tear dry eye
author_facet	Eun Hye Kim Eun-Song Lee Dong Yun Lee Young-Pil Kim
author_sort	Eun Hye Kim
title	Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes
title_short	Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes
title_full	Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes
title_fullStr	Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes
title_full_unstemmed	Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes
title_sort	facile determination of sodium ion and osmolarity in artificial tears by sequential dnazymes
publisher	MDPI AG
series	Sensors
issn	1424-8220
publishDate	2017-12-01
description	Despite high relevance of tear osmolarity and eye abnormality, numerous methods for detecting tear osmolarity rely upon expensive osmometers. We report a reliable method for simply determining sodium ion-based osmolarity in artificial tears using sequential DNAzymes. When sodium ion-specific DNAzyme and peroxidase-like DNAzyme were used as a sensing and detecting probe, respectively, the concentration of Na+ in artificial tears could be measured by absorbance or fluorescence intensity, which was highly correlated with osmolarity over the diagnostic range (R2 > 0.98). Our approach is useful for studying eye diseases in relation to osmolarity.
topic	DNAzyme osmolarity sodium ion salinity tear dry eye
url	https://www.mdpi.com/1424-8220/17/12/2840
work_keys_str_mv	AT eunhyekim faciledeterminationofsodiumionandosmolarityinartificialtearsbysequentialdnazymes AT eunsonglee faciledeterminationofsodiumionandosmolarityinartificialtearsbysequentialdnazymes AT dongyunlee faciledeterminationofsodiumionandosmolarityinartificialtearsbysequentialdnazymes AT youngpilkim faciledeterminationofsodiumionandosmolarityinartificialtearsbysequentialdnazymes
_version_	1716812541214064640

Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes

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