Epigallocatechin-3-gallate and Epigallocatechin-3-O-(3-O-methyl)-gallate Enhance the Bonding Stability of an Etch-and-Rinse Adhesive to Dentin
This study evaluated epigallocatechin-3-gallate (EGCG) and epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG-3Me) modified etch-and-rinse adhesives (Single Bond 2, SB 2) for their antibacterial effect and bonding stability to dentin. EGCG-3Me was isolated and purified with column chromatography and pr...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2017-02-01
|
Series: | Materials |
Subjects: | |
Online Access: | http://www.mdpi.com/1996-1944/10/2/183 |
id |
doaj-e37c8e5a4fa1475ca052345fdb88dacf |
---|---|
record_format |
Article |
spelling |
doaj-e37c8e5a4fa1475ca052345fdb88dacf2020-11-24T20:58:07ZengMDPI AGMaterials1996-19442017-02-0110218310.3390/ma10020183ma10020183Epigallocatechin-3-gallate and Epigallocatechin-3-O-(3-O-methyl)-gallate Enhance the Bonding Stability of an Etch-and-Rinse Adhesive to DentinHao-Han Yu0Ling Zhang1Fan Yu2Fang Li3Zheng-Ya Liu4Ji-Hua Chen5State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, 145 West Changle Road, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, 145 West Changle Road, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, 145 West Changle Road, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, 145 West Changle Road, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, 145 West Changle Road, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, 145 West Changle Road, Xi’an 710032, ChinaThis study evaluated epigallocatechin-3-gallate (EGCG) and epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG-3Me) modified etch-and-rinse adhesives (Single Bond 2, SB 2) for their antibacterial effect and bonding stability to dentin. EGCG-3Me was isolated and purified with column chromatography and preparative high performance liquid chromatography. EGCG and EGCG-3Me were incorporated separately into the adhesive SB 2 at concentrations of 200, 400, and 600 µg/mL. The effect of cured adhesives on the growth of Streptococcus mutans (S. mutans) was determined with scanning electron microscopy and confocal laser scanning microscopy; the biofilm of bacteria was further quantified via optical density 600 values. The inhibition of EGCG and EGCG-3Me on dentin-originated collagen proteases activities was evaluated with a proteases fluorometric assay kit. The degree of conversion (DC) of the adhesives was tested with micro-Raman spectrum. The immediate and post-thermocycling (5000 cycles) bond strength was assessed through Microtensile Bond Strength (MTBS) test. Cured EGCG/EGCG-3Me modified adhesives inhibit the growth of S. mutans in a concentration-dependent manner. The immediate MTBS of SB 2 was not compromised by EGCG/EGCG-3Me modification. EGCG/EGCG-3Me modified adhesive had higher MTBS than SB 2 after thermocycling, showing no correlation with concentration. The DC of the adhesive system was affected depending on the concentration of EGCG/EGCG-3Me and the depth of the hybrid layer. EGCG/EGCG-3Me modified adhesives could inhibit S. mutans adhesion to dentin–resin interface, and maintain the bonding stability. The adhesive modified with 400 µg/mL EGCG-3Me showed antibacterial effect and enhanced bonding stability without affect the DC of adhesive.http://www.mdpi.com/1996-1944/10/2/183antibacterial effectdental adhesiveepigallocatechin-3-gallateepigallocatechin-3-O-(3-O-methyl)-gallatemicrotensile bond strengththermocycling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hao-Han Yu Ling Zhang Fan Yu Fang Li Zheng-Ya Liu Ji-Hua Chen |
spellingShingle |
Hao-Han Yu Ling Zhang Fan Yu Fang Li Zheng-Ya Liu Ji-Hua Chen Epigallocatechin-3-gallate and Epigallocatechin-3-O-(3-O-methyl)-gallate Enhance the Bonding Stability of an Etch-and-Rinse Adhesive to Dentin Materials antibacterial effect dental adhesive epigallocatechin-3-gallate epigallocatechin-3-O-(3-O-methyl)-gallate microtensile bond strength thermocycling |
author_facet |
Hao-Han Yu Ling Zhang Fan Yu Fang Li Zheng-Ya Liu Ji-Hua Chen |
author_sort |
Hao-Han Yu |
title |
Epigallocatechin-3-gallate and Epigallocatechin-3-O-(3-O-methyl)-gallate Enhance the Bonding Stability of an Etch-and-Rinse Adhesive to Dentin |
title_short |
Epigallocatechin-3-gallate and Epigallocatechin-3-O-(3-O-methyl)-gallate Enhance the Bonding Stability of an Etch-and-Rinse Adhesive to Dentin |
title_full |
Epigallocatechin-3-gallate and Epigallocatechin-3-O-(3-O-methyl)-gallate Enhance the Bonding Stability of an Etch-and-Rinse Adhesive to Dentin |
title_fullStr |
Epigallocatechin-3-gallate and Epigallocatechin-3-O-(3-O-methyl)-gallate Enhance the Bonding Stability of an Etch-and-Rinse Adhesive to Dentin |
title_full_unstemmed |
Epigallocatechin-3-gallate and Epigallocatechin-3-O-(3-O-methyl)-gallate Enhance the Bonding Stability of an Etch-and-Rinse Adhesive to Dentin |
title_sort |
epigallocatechin-3-gallate and epigallocatechin-3-o-(3-o-methyl)-gallate enhance the bonding stability of an etch-and-rinse adhesive to dentin |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2017-02-01 |
description |
This study evaluated epigallocatechin-3-gallate (EGCG) and epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG-3Me) modified etch-and-rinse adhesives (Single Bond 2, SB 2) for their antibacterial effect and bonding stability to dentin. EGCG-3Me was isolated and purified with column chromatography and preparative high performance liquid chromatography. EGCG and EGCG-3Me were incorporated separately into the adhesive SB 2 at concentrations of 200, 400, and 600 µg/mL. The effect of cured adhesives on the growth of Streptococcus mutans (S. mutans) was determined with scanning electron microscopy and confocal laser scanning microscopy; the biofilm of bacteria was further quantified via optical density 600 values. The inhibition of EGCG and EGCG-3Me on dentin-originated collagen proteases activities was evaluated with a proteases fluorometric assay kit. The degree of conversion (DC) of the adhesives was tested with micro-Raman spectrum. The immediate and post-thermocycling (5000 cycles) bond strength was assessed through Microtensile Bond Strength (MTBS) test. Cured EGCG/EGCG-3Me modified adhesives inhibit the growth of S. mutans in a concentration-dependent manner. The immediate MTBS of SB 2 was not compromised by EGCG/EGCG-3Me modification. EGCG/EGCG-3Me modified adhesive had higher MTBS than SB 2 after thermocycling, showing no correlation with concentration. The DC of the adhesive system was affected depending on the concentration of EGCG/EGCG-3Me and the depth of the hybrid layer. EGCG/EGCG-3Me modified adhesives could inhibit S. mutans adhesion to dentin–resin interface, and maintain the bonding stability. The adhesive modified with 400 µg/mL EGCG-3Me showed antibacterial effect and enhanced bonding stability without affect the DC of adhesive. |
topic |
antibacterial effect dental adhesive epigallocatechin-3-gallate epigallocatechin-3-O-(3-O-methyl)-gallate microtensile bond strength thermocycling |
url |
http://www.mdpi.com/1996-1944/10/2/183 |
work_keys_str_mv |
AT haohanyu epigallocatechin3gallateandepigallocatechin3o3omethylgallateenhancethebondingstabilityofanetchandrinseadhesivetodentin AT lingzhang epigallocatechin3gallateandepigallocatechin3o3omethylgallateenhancethebondingstabilityofanetchandrinseadhesivetodentin AT fanyu epigallocatechin3gallateandepigallocatechin3o3omethylgallateenhancethebondingstabilityofanetchandrinseadhesivetodentin AT fangli epigallocatechin3gallateandepigallocatechin3o3omethylgallateenhancethebondingstabilityofanetchandrinseadhesivetodentin AT zhengyaliu epigallocatechin3gallateandepigallocatechin3o3omethylgallateenhancethebondingstabilityofanetchandrinseadhesivetodentin AT jihuachen epigallocatechin3gallateandepigallocatechin3o3omethylgallateenhancethebondingstabilityofanetchandrinseadhesivetodentin |
_version_ |
1716786566486032384 |