Alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons
The cochlear implant outcome is possibly improved by brain-derived neurotrophic factor treatment protecting spiral ganglion neurons. Implantation of genetically modified mesenchymal stem cells may enable the required long-term brain-derived neurotrophic factor administration. Encapsulation of mesenc...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SAGE Publishing
2020-04-01
|
Series: | Journal of Tissue Engineering |
Online Access: | https://doi.org/10.1177/2041731420911313 |
id |
doaj-e38a5a6e280c4ce69be67c4e7bb61741 |
---|---|
record_format |
Article |
spelling |
doaj-e38a5a6e280c4ce69be67c4e7bb617412020-11-25T03:04:41ZengSAGE PublishingJournal of Tissue Engineering2041-73142020-04-011110.1177/2041731420911313Alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neuronsJana Schwieger0Anika Hamm1Michael M. Gepp2André Schulz3Andrea Hoffmann4Thomas Lenarz5Verena Scheper6NIFE—Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, GermanyDepartment of Orthopaedic Surgery, Hannover Medical School, Hannover, GermanyFraunhofer Project Center for Stem Cell Process Engineering, Würzburg, GermanyFraunhofer Institute for Biomedical Engineering IBMT, Sulzbach, GermanyDepartment of Orthopaedic Surgery, Hannover Medical School, Hannover, GermanyCluster of Excellence Hearing4all, German Research Foundation, Hannover, GermanyCluster of Excellence Hearing4all, German Research Foundation, Hannover, GermanyThe cochlear implant outcome is possibly improved by brain-derived neurotrophic factor treatment protecting spiral ganglion neurons. Implantation of genetically modified mesenchymal stem cells may enable the required long-term brain-derived neurotrophic factor administration. Encapsulation of mesenchymal stem cells in ultra-high viscous alginate may protect the mesenchymal stem cells from the recipient’s immune system and prevent their uncontrolled migration. Alginate stability and survival of mesenchymal stem cells in alginate were evaluated. Brain-derived neurotrophic factor production was measured and its protective effect was analyzed in dissociated rat spiral ganglion neuron co-culture. Since the cochlear implant is an active electrode, alginate–mesenchymal stem cell samples were electrically stimulated and alginate stability and mesenchymal stem cell survival were investigated. Stability of ultra-high viscous-alginate and alginate–mesenchymal stem cells was proven. Brain-derived neurotrophic factor production was detectable and spiral ganglion neuron survival, bipolar morphology, and neurite outgrowth were increased. Moderate electrical stimulation did not affect the mesenchymal stem cell survival and their viability was good within the investigated time frame. Local drug delivery by ultra-high viscous-alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells is a promising strategy to improve the cochlear implant outcome.https://doi.org/10.1177/2041731420911313 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jana Schwieger Anika Hamm Michael M. Gepp André Schulz Andrea Hoffmann Thomas Lenarz Verena Scheper |
spellingShingle |
Jana Schwieger Anika Hamm Michael M. Gepp André Schulz Andrea Hoffmann Thomas Lenarz Verena Scheper Alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons Journal of Tissue Engineering |
author_facet |
Jana Schwieger Anika Hamm Michael M. Gepp André Schulz Andrea Hoffmann Thomas Lenarz Verena Scheper |
author_sort |
Jana Schwieger |
title |
Alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons |
title_short |
Alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons |
title_full |
Alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons |
title_fullStr |
Alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons |
title_full_unstemmed |
Alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons |
title_sort |
alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons |
publisher |
SAGE Publishing |
series |
Journal of Tissue Engineering |
issn |
2041-7314 |
publishDate |
2020-04-01 |
description |
The cochlear implant outcome is possibly improved by brain-derived neurotrophic factor treatment protecting spiral ganglion neurons. Implantation of genetically modified mesenchymal stem cells may enable the required long-term brain-derived neurotrophic factor administration. Encapsulation of mesenchymal stem cells in ultra-high viscous alginate may protect the mesenchymal stem cells from the recipient’s immune system and prevent their uncontrolled migration. Alginate stability and survival of mesenchymal stem cells in alginate were evaluated. Brain-derived neurotrophic factor production was measured and its protective effect was analyzed in dissociated rat spiral ganglion neuron co-culture. Since the cochlear implant is an active electrode, alginate–mesenchymal stem cell samples were electrically stimulated and alginate stability and mesenchymal stem cell survival were investigated. Stability of ultra-high viscous-alginate and alginate–mesenchymal stem cells was proven. Brain-derived neurotrophic factor production was detectable and spiral ganglion neuron survival, bipolar morphology, and neurite outgrowth were increased. Moderate electrical stimulation did not affect the mesenchymal stem cell survival and their viability was good within the investigated time frame. Local drug delivery by ultra-high viscous-alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells is a promising strategy to improve the cochlear implant outcome. |
url |
https://doi.org/10.1177/2041731420911313 |
work_keys_str_mv |
AT janaschwieger alginateencapsulatedbrainderivedneurotrophicfactoroverexpressingmesenchymalstemcellsareapromisingdrugdeliverysystemforprotectionofauditoryneurons AT anikahamm alginateencapsulatedbrainderivedneurotrophicfactoroverexpressingmesenchymalstemcellsareapromisingdrugdeliverysystemforprotectionofauditoryneurons AT michaelmgepp alginateencapsulatedbrainderivedneurotrophicfactoroverexpressingmesenchymalstemcellsareapromisingdrugdeliverysystemforprotectionofauditoryneurons AT andreschulz alginateencapsulatedbrainderivedneurotrophicfactoroverexpressingmesenchymalstemcellsareapromisingdrugdeliverysystemforprotectionofauditoryneurons AT andreahoffmann alginateencapsulatedbrainderivedneurotrophicfactoroverexpressingmesenchymalstemcellsareapromisingdrugdeliverysystemforprotectionofauditoryneurons AT thomaslenarz alginateencapsulatedbrainderivedneurotrophicfactoroverexpressingmesenchymalstemcellsareapromisingdrugdeliverysystemforprotectionofauditoryneurons AT verenascheper alginateencapsulatedbrainderivedneurotrophicfactoroverexpressingmesenchymalstemcellsareapromisingdrugdeliverysystemforprotectionofauditoryneurons |
_version_ |
1724680359513161728 |