Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures
The study aims to identify the phenotypic marker expressions of different human adult stem cells derived from, namely, bone marrow, subcutaneous fat, and omentum fat, cultured in different media, namely, DMEM-Low Glucose, Alpha-MEM, DMEM-F12 and DMEM-KO and under long term culture conditions (>P2...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2015-01-01
|
Series: | Stem Cells International |
Online Access: | http://dx.doi.org/10.1155/2015/146051 |
id |
doaj-e38e00000bc24bf2bccc96cf599c0092 |
---|---|
record_format |
Article |
spelling |
doaj-e38e00000bc24bf2bccc96cf599c00922020-11-24T20:59:08ZengHindawi LimitedStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/146051146051Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term CulturesIndumathi Somasundaram0Rashmi Mishra1Harikrishnan Radhakrishnan2Rajkumar Sankaran3Venkata Naga Srikanth Garikipati4Dhanasekaran Marappagounder5Department of Stem Cells, National Institute of Nutrition, Secunderabad 500 007, IndiaInstitute for Biochemistry and Molecular Biology, Ulm University, M24, Level 3, Room 358, Albert-Einstein-Allee 11, 89081 Ulm, GermanyBerlin School of Integrative Oncology, Buch, 131254 Berlin, GermanyLifeline RIGID Hospitals, Chennai 600 010, IndiaStem Cell Therapy Program, Center for Translational Medicine, Temple University School of Medicine, Temple University, Room MERB 9-943, 3500 North Broad Street, Philadelphia, PA 19104, USARee Laboratories Private Limited, Andheri West, Mumbai 400 053, IndiaThe study aims to identify the phenotypic marker expressions of different human adult stem cells derived from, namely, bone marrow, subcutaneous fat, and omentum fat, cultured in different media, namely, DMEM-Low Glucose, Alpha-MEM, DMEM-F12 and DMEM-KO and under long term culture conditions (>P20). We characterized immunophenotype by using various hematopoietic, mesenchymal, endothelial markers, and cell adhesion molecules in the long term cultures (Passages-P1, P3, P5, P9, P12, P15, and P20.) Interestingly, data revealed similar marker expression profiles irrespective of source, basal media, and extensive culturing. This demonstrates that all adult stem cell sources mentioned in this study share similar phenotypic marker and all media seem appropriate for culturing these sources. However, a disparity was observed in the markers such as CD49d, CD54, CD117, CD29, and CD106, thereby warranting further research on these markers. Besides the aforesaid objective, it is understood from the study that immunophenotyping acts as a valuable tool to identify inherent property of each cell, thereby leading to a valuable cell based therapy.http://dx.doi.org/10.1155/2015/146051 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Indumathi Somasundaram Rashmi Mishra Harikrishnan Radhakrishnan Rajkumar Sankaran Venkata Naga Srikanth Garikipati Dhanasekaran Marappagounder |
spellingShingle |
Indumathi Somasundaram Rashmi Mishra Harikrishnan Radhakrishnan Rajkumar Sankaran Venkata Naga Srikanth Garikipati Dhanasekaran Marappagounder Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures Stem Cells International |
author_facet |
Indumathi Somasundaram Rashmi Mishra Harikrishnan Radhakrishnan Rajkumar Sankaran Venkata Naga Srikanth Garikipati Dhanasekaran Marappagounder |
author_sort |
Indumathi Somasundaram |
title |
Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures |
title_short |
Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures |
title_full |
Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures |
title_fullStr |
Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures |
title_full_unstemmed |
Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures |
title_sort |
human adult stem cells maintain a constant phenotype profile irrespective of their origin, basal media, and long term cultures |
publisher |
Hindawi Limited |
series |
Stem Cells International |
issn |
1687-966X 1687-9678 |
publishDate |
2015-01-01 |
description |
The study aims to identify the phenotypic marker expressions of different human adult stem cells derived from, namely, bone marrow, subcutaneous fat, and omentum fat, cultured in different media, namely, DMEM-Low Glucose, Alpha-MEM, DMEM-F12 and DMEM-KO and under long term culture conditions (>P20). We characterized immunophenotype by using various hematopoietic, mesenchymal, endothelial markers, and cell adhesion molecules in the long term cultures (Passages-P1, P3, P5, P9, P12, P15, and P20.) Interestingly, data revealed similar marker expression profiles irrespective of source, basal media, and extensive culturing. This demonstrates that all adult stem cell sources mentioned in this study share similar phenotypic marker and all media seem appropriate for culturing these sources. However, a disparity was observed in the markers such as CD49d, CD54, CD117, CD29, and CD106, thereby warranting further research on these markers. Besides the aforesaid objective, it is understood from the study that immunophenotyping acts as a valuable tool to identify inherent property of each cell, thereby leading to a valuable cell based therapy. |
url |
http://dx.doi.org/10.1155/2015/146051 |
work_keys_str_mv |
AT indumathisomasundaram humanadultstemcellsmaintainaconstantphenotypeprofileirrespectiveoftheiroriginbasalmediaandlongtermcultures AT rashmimishra humanadultstemcellsmaintainaconstantphenotypeprofileirrespectiveoftheiroriginbasalmediaandlongtermcultures AT harikrishnanradhakrishnan humanadultstemcellsmaintainaconstantphenotypeprofileirrespectiveoftheiroriginbasalmediaandlongtermcultures AT rajkumarsankaran humanadultstemcellsmaintainaconstantphenotypeprofileirrespectiveoftheiroriginbasalmediaandlongtermcultures AT venkatanagasrikanthgarikipati humanadultstemcellsmaintainaconstantphenotypeprofileirrespectiveoftheiroriginbasalmediaandlongtermcultures AT dhanasekaranmarappagounder humanadultstemcellsmaintainaconstantphenotypeprofileirrespectiveoftheiroriginbasalmediaandlongtermcultures |
_version_ |
1716783603799556096 |