SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress
Abstract Background The sucrose non-fermenting 1-related kinases 2 (SnRK2s) play important roles in osmotic stress responses in A. thaliana and rice (Oryza sativa L.). Osmotic stress/ABA–activated protein kinase 2 (SAPK2) is a member of SnRK2s subclass II in rice, but its function in rice yield unde...
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doaj-e3974300b1ad458d96677d1b781345222020-11-25T04:04:24ZengSpringerOpenRice1939-84251939-84332020-06-0113111210.1186/s12284-020-00395-3SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stressDengji Lou0Zhen Chen1Diqiu Yu2Xiaoyan Yang3School of Chemical, Biological and Environmental Sciences, Yuxi Normal UniversitySchool of Chemical, Biological and Environmental Sciences, Yuxi Normal UniversityState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan UniversityFaculty of Life Science and Technology, Kunming University of Science and TechnologyAbstract Background The sucrose non-fermenting 1-related kinases 2 (SnRK2s) play important roles in osmotic stress responses in A. thaliana and rice (Oryza sativa L.). Osmotic stress/ABA–activated protein kinase 2 (SAPK2) is a member of SnRK2s subclass II in rice, but its function in rice yield under drought stress is unclear. Results Compared with wild-type (Oryza.Sativa L.spp.japonica, WT) plants, the sapk2 rice mutant lines were shorter and produced fewer grains per panicle, smaller grains and lower grain yield under reproductive stage drought stress (RDS). Subsequent analysis suggested that SAPK2 considerably influences the nitrogen, phosphorus, and potassium contents of rice grains. The examination of rice seedling growth and development under nutrient-deprived conditions (−N, −K, and − P) proved that SAPK2 can significantly affect rice seedling growth and root development in hydroponic cultures lacking N and K. Moreover, the NO3 − influx rate and nitrate concentration analysis indicated that SAPK2 promotes nitrate uptake and assimilation by regulating nitrate-related transporters. Conclusion These results suggest that SAPK2 could enhance grain production by regulating nitrogen utilization efficiency under RDS. Our work provided insights to breeding drought tolerant rice with high nutrient uptake.http://link.springer.com/article/10.1186/s12284-020-00395-3RiceSAPK2Grain yieldGrain sizeNO3 − influxDrought stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dengji Lou Zhen Chen Diqiu Yu Xiaoyan Yang |
spellingShingle |
Dengji Lou Zhen Chen Diqiu Yu Xiaoyan Yang SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress Rice Rice SAPK2 Grain yield Grain size NO3 − influx Drought stress |
author_facet |
Dengji Lou Zhen Chen Diqiu Yu Xiaoyan Yang |
author_sort |
Dengji Lou |
title |
SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress |
title_short |
SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress |
title_full |
SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress |
title_fullStr |
SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress |
title_full_unstemmed |
SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress |
title_sort |
sapk2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress |
publisher |
SpringerOpen |
series |
Rice |
issn |
1939-8425 1939-8433 |
publishDate |
2020-06-01 |
description |
Abstract Background The sucrose non-fermenting 1-related kinases 2 (SnRK2s) play important roles in osmotic stress responses in A. thaliana and rice (Oryza sativa L.). Osmotic stress/ABA–activated protein kinase 2 (SAPK2) is a member of SnRK2s subclass II in rice, but its function in rice yield under drought stress is unclear. Results Compared with wild-type (Oryza.Sativa L.spp.japonica, WT) plants, the sapk2 rice mutant lines were shorter and produced fewer grains per panicle, smaller grains and lower grain yield under reproductive stage drought stress (RDS). Subsequent analysis suggested that SAPK2 considerably influences the nitrogen, phosphorus, and potassium contents of rice grains. The examination of rice seedling growth and development under nutrient-deprived conditions (−N, −K, and − P) proved that SAPK2 can significantly affect rice seedling growth and root development in hydroponic cultures lacking N and K. Moreover, the NO3 − influx rate and nitrate concentration analysis indicated that SAPK2 promotes nitrate uptake and assimilation by regulating nitrate-related transporters. Conclusion These results suggest that SAPK2 could enhance grain production by regulating nitrogen utilization efficiency under RDS. Our work provided insights to breeding drought tolerant rice with high nutrient uptake. |
topic |
Rice SAPK2 Grain yield Grain size NO3 − influx Drought stress |
url |
http://link.springer.com/article/10.1186/s12284-020-00395-3 |
work_keys_str_mv |
AT dengjilou sapk2contributestoriceyieldbymodulatingnitrogenmetabolicprocessesunderreproductivestagedroughtstress AT zhenchen sapk2contributestoriceyieldbymodulatingnitrogenmetabolicprocessesunderreproductivestagedroughtstress AT diqiuyu sapk2contributestoriceyieldbymodulatingnitrogenmetabolicprocessesunderreproductivestagedroughtstress AT xiaoyanyang sapk2contributestoriceyieldbymodulatingnitrogenmetabolicprocessesunderreproductivestagedroughtstress |
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1724436960357908480 |