Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae
Abstract Background Anopheles sinensis is a dominant natural vector of Plasmodium vivax in China, Taiwan, Japan, and Korea. Recent genome sequencing of An. sinensis provides important insights into the genomic basis of vectorial capacity. However, the lack of a physical genome map with chromosome as...
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doaj-e39d2f736fec439f97391627ec18db622020-11-24T21:03:00ZengBMCMalaria Journal1475-28752017-06-0116111010.1186/s12936-017-1888-7Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiaeYun Wei0Biao Cheng1Guoding Zhu2Danyu Shen3Jiangtao Liang4Cong Wang5Jing Wang6Jianxia Tang7Jun Cao8Igor V. Sharakhov9Ai Xia10Department of Entomology, Nanjing Agricultural UniversityDepartment of Entomology, Nanjing Agricultural UniversityKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic DiseasesDepartment of Entomology, Nanjing Agricultural UniversityDepartment of Entomology, Nanjing Agricultural UniversityDepartment of Entomology, Nanjing Agricultural UniversityDepartment of Entomology, Nanjing Agricultural UniversityKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic DiseasesKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic DiseasesDepartment of Entomology, Fralin Life Science Institute, Virginia TechDepartment of Entomology, Nanjing Agricultural UniversityAbstract Background Anopheles sinensis is a dominant natural vector of Plasmodium vivax in China, Taiwan, Japan, and Korea. Recent genome sequencing of An. sinensis provides important insights into the genomic basis of vectorial capacity. However, the lack of a physical genome map with chromosome assignment and orientation of sequencing scaffolds hinders comparative analyses with other genomes to infer evolutionary changes relevant to the vector capacity. Results Here, a physical genome map for An. sinensis was constructed by assigning 52 scaffolds onto the chromosomes using fluorescence in situ hybridization (FISH). This chromosome-based genome assembly composes approximately 36% of the total An. sinensis genome. Comparisons of 3955 orthologous genes between An. sinensis and Anopheles gambiae identified 361 conserved synteny blocks and 267 inversions fixed between these two lineages. The rate of gene order reshuffling on the X chromosome is approximately 3.2 times higher than that on the autosomes. Conclusions The physical map will facilitate detailed genomic analysis of An. sinensis and contribute to understanding of the patterns and mechanisms of large-scale genome rearrangements in anopheline mosquitoes.http://link.springer.com/article/10.1186/s12936-017-1888-7Chromosomal evolutionGRIMMInversion fixationFluorescence in situ hybridizationOrthoDBSynteny blocks |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yun Wei Biao Cheng Guoding Zhu Danyu Shen Jiangtao Liang Cong Wang Jing Wang Jianxia Tang Jun Cao Igor V. Sharakhov Ai Xia |
spellingShingle |
Yun Wei Biao Cheng Guoding Zhu Danyu Shen Jiangtao Liang Cong Wang Jing Wang Jianxia Tang Jun Cao Igor V. Sharakhov Ai Xia Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae Malaria Journal Chromosomal evolution GRIMM Inversion fixation Fluorescence in situ hybridization OrthoDB Synteny blocks |
author_facet |
Yun Wei Biao Cheng Guoding Zhu Danyu Shen Jiangtao Liang Cong Wang Jing Wang Jianxia Tang Jun Cao Igor V. Sharakhov Ai Xia |
author_sort |
Yun Wei |
title |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_short |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_full |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_fullStr |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_full_unstemmed |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_sort |
comparative physical genome mapping of malaria vectors anopheles sinensis and anopheles gambiae |
publisher |
BMC |
series |
Malaria Journal |
issn |
1475-2875 |
publishDate |
2017-06-01 |
description |
Abstract Background Anopheles sinensis is a dominant natural vector of Plasmodium vivax in China, Taiwan, Japan, and Korea. Recent genome sequencing of An. sinensis provides important insights into the genomic basis of vectorial capacity. However, the lack of a physical genome map with chromosome assignment and orientation of sequencing scaffolds hinders comparative analyses with other genomes to infer evolutionary changes relevant to the vector capacity. Results Here, a physical genome map for An. sinensis was constructed by assigning 52 scaffolds onto the chromosomes using fluorescence in situ hybridization (FISH). This chromosome-based genome assembly composes approximately 36% of the total An. sinensis genome. Comparisons of 3955 orthologous genes between An. sinensis and Anopheles gambiae identified 361 conserved synteny blocks and 267 inversions fixed between these two lineages. The rate of gene order reshuffling on the X chromosome is approximately 3.2 times higher than that on the autosomes. Conclusions The physical map will facilitate detailed genomic analysis of An. sinensis and contribute to understanding of the patterns and mechanisms of large-scale genome rearrangements in anopheline mosquitoes. |
topic |
Chromosomal evolution GRIMM Inversion fixation Fluorescence in situ hybridization OrthoDB Synteny blocks |
url |
http://link.springer.com/article/10.1186/s12936-017-1888-7 |
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