Biomathematical description of synthetic peptide libraries.
Libraries of randomised peptides displayed on phages or viral particles are essential tools in a wide spectrum of applications. However, there is only limited understanding of a library's fundamental dynamics and the influences of encoding schemes and sizes on their quality. Numeric properties...
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2015-01-01
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doaj-e3a31ae20b9b4cf788d39cc08696bb712020-11-25T01:53:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012920010.1371/journal.pone.0129200Biomathematical description of synthetic peptide libraries.Timo SieberEric HareHeike HofmannMartin TrepelLibraries of randomised peptides displayed on phages or viral particles are essential tools in a wide spectrum of applications. However, there is only limited understanding of a library's fundamental dynamics and the influences of encoding schemes and sizes on their quality. Numeric properties of libraries, such as the expected number of different peptides and the library's coverage, have long been in use as measures of a library's quality. Here, we present a graphical framework of these measures together with a library's relative efficiency to help to describe libraries in enough detail for researchers to plan new experiments in a more informed manner. In particular, these values allow us to answer-in a probabilistic fashion-the question of whether a specific library does indeed contain one of the "best" possible peptides. The framework is implemented in a web-interface based on two packages, discreteRV and peptider, to the statistical software environment R. We further provide a user-friendly web-interface called PeLiCa (Peptide Library Calculator, http://www.pelica.org), allowing scientists to plan and analyse their peptide libraries.http://europepmc.org/articles/PMC4456392?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Timo Sieber Eric Hare Heike Hofmann Martin Trepel |
spellingShingle |
Timo Sieber Eric Hare Heike Hofmann Martin Trepel Biomathematical description of synthetic peptide libraries. PLoS ONE |
author_facet |
Timo Sieber Eric Hare Heike Hofmann Martin Trepel |
author_sort |
Timo Sieber |
title |
Biomathematical description of synthetic peptide libraries. |
title_short |
Biomathematical description of synthetic peptide libraries. |
title_full |
Biomathematical description of synthetic peptide libraries. |
title_fullStr |
Biomathematical description of synthetic peptide libraries. |
title_full_unstemmed |
Biomathematical description of synthetic peptide libraries. |
title_sort |
biomathematical description of synthetic peptide libraries. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Libraries of randomised peptides displayed on phages or viral particles are essential tools in a wide spectrum of applications. However, there is only limited understanding of a library's fundamental dynamics and the influences of encoding schemes and sizes on their quality. Numeric properties of libraries, such as the expected number of different peptides and the library's coverage, have long been in use as measures of a library's quality. Here, we present a graphical framework of these measures together with a library's relative efficiency to help to describe libraries in enough detail for researchers to plan new experiments in a more informed manner. In particular, these values allow us to answer-in a probabilistic fashion-the question of whether a specific library does indeed contain one of the "best" possible peptides. The framework is implemented in a web-interface based on two packages, discreteRV and peptider, to the statistical software environment R. We further provide a user-friendly web-interface called PeLiCa (Peptide Library Calculator, http://www.pelica.org), allowing scientists to plan and analyse their peptide libraries. |
url |
http://europepmc.org/articles/PMC4456392?pdf=render |
work_keys_str_mv |
AT timosieber biomathematicaldescriptionofsyntheticpeptidelibraries AT erichare biomathematicaldescriptionofsyntheticpeptidelibraries AT heikehofmann biomathematicaldescriptionofsyntheticpeptidelibraries AT martintrepel biomathematicaldescriptionofsyntheticpeptidelibraries |
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