Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers
Au- and iron-based magnetic nanoparticles (NPs) are promising NPs for biomedical applications due to their unique properties. The combination of a gold coating over a magnetic core puts together the benefits from adding the magnetic properties to the robust chemistry provided by the thiol functional...
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doaj-e3ad694ed3e243d9b650a9e2bef043492020-11-24T23:46:04ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-11-011611276252763910.3390/ijms161126046ijms161126046Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA AptamersMaria Tintoré0Stefania Mazzini1Laura Polito2Marcello Marelli3Alfonso Latorre4Álvaro Somoza5Anna Aviñó6Carme Fàbrega7Ramon Eritja8Department of Chemical and Biomolecular Nanotechnology, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, C/Jordi Girona 18-26, 08034 Barcelona, SpainDepartment of Food, Environmental and Nutritional Sciences (DEFENS), Division of Chemistry and Molecular Biology, University of Milan, Via Celoria 2, 20133 Milan, ItalyDepartment Institute of Molecular Science and Technologies, ISTM-CNR, Via G. Fantoli 16/15, 20138 Milan, ItalyDepartment Institute of Molecular Science and Technologies, ISTM-CNR, Via G. Fantoli 16/15, 20138 Milan, ItalyIMDEA Nanociencia & Nanobiotecnología (IMDEA-Nanociencia), Asociada al Centro Nacional de Biotecnología (CSIC), C/Faraday 9, 28049 Madrid, SpainIMDEA Nanociencia & Nanobiotecnología (IMDEA-Nanociencia), Asociada al Centro Nacional de Biotecnología (CSIC), C/Faraday 9, 28049 Madrid, SpainDepartment of Chemical and Biomolecular Nanotechnology, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, C/Jordi Girona 18-26, 08034 Barcelona, SpainDepartment of Chemical and Biomolecular Nanotechnology, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, C/Jordi Girona 18-26, 08034 Barcelona, SpainDepartment of Chemical and Biomolecular Nanotechnology, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, C/Jordi Girona 18-26, 08034 Barcelona, SpainAu- and iron-based magnetic nanoparticles (NPs) are promising NPs for biomedical applications due to their unique properties. The combination of a gold coating over a magnetic core puts together the benefits from adding the magnetic properties to the robust chemistry provided by the thiol functionalization of gold. Here, the use of Au-coated magnetic NPs for molecular detection of a single methylation in DNA aptamer is described. Binding of α-thrombin to two aptamers conjugated to these NPs causes aggregation, a phenomenon that can be observed by UV, DLS and MRI. These techniques discriminate a single methylation in one of the aptamers, preventing aggregation due to the inability of α-thrombin to recognize it. A parallel study with gold and ferromagnetic NPs is detailed, concluding that the Au coating of FexOy NP does not affect their performance and that they are suitable as complex biosensors. These results prove the high detection potency of Au-coated SPIONs for biomedical applications especially for DNA repair detection.http://www.mdpi.com/1422-0067/16/11/26046biosensorgold-coated superparamagnetic nanoparticlesnanotechnologyaptamers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Tintoré Stefania Mazzini Laura Polito Marcello Marelli Alfonso Latorre Álvaro Somoza Anna Aviñó Carme Fàbrega Ramon Eritja |
spellingShingle |
Maria Tintoré Stefania Mazzini Laura Polito Marcello Marelli Alfonso Latorre Álvaro Somoza Anna Aviñó Carme Fàbrega Ramon Eritja Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers International Journal of Molecular Sciences biosensor gold-coated superparamagnetic nanoparticles nanotechnology aptamers |
author_facet |
Maria Tintoré Stefania Mazzini Laura Polito Marcello Marelli Alfonso Latorre Álvaro Somoza Anna Aviñó Carme Fàbrega Ramon Eritja |
author_sort |
Maria Tintoré |
title |
Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers |
title_short |
Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers |
title_full |
Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers |
title_fullStr |
Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers |
title_full_unstemmed |
Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers |
title_sort |
gold-coated superparamagnetic nanoparticles for single methyl discrimination in dna aptamers |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2015-11-01 |
description |
Au- and iron-based magnetic nanoparticles (NPs) are promising NPs for biomedical applications due to their unique properties. The combination of a gold coating over a magnetic core puts together the benefits from adding the magnetic properties to the robust chemistry provided by the thiol functionalization of gold. Here, the use of Au-coated magnetic NPs for molecular detection of a single methylation in DNA aptamer is described. Binding of α-thrombin to two aptamers conjugated to these NPs causes aggregation, a phenomenon that can be observed by UV, DLS and MRI. These techniques discriminate a single methylation in one of the aptamers, preventing aggregation due to the inability of α-thrombin to recognize it. A parallel study with gold and ferromagnetic NPs is detailed, concluding that the Au coating of FexOy NP does not affect their performance and that they are suitable as complex biosensors. These results prove the high detection potency of Au-coated SPIONs for biomedical applications especially for DNA repair detection. |
topic |
biosensor gold-coated superparamagnetic nanoparticles nanotechnology aptamers |
url |
http://www.mdpi.com/1422-0067/16/11/26046 |
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