Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats
In the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of wild grape seed procyanidins (WGP) against oxidative stress damage in ethanol-treated HepG2 cell and Sprague-Dawley (SD)-rat models. In HepG2 cells, WGP not only diminished the ethanol (EtOH, 100 mM)...
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doaj-e3af03466ce64b13a4b70420e301abef2020-11-24T21:45:06ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-05-0117575810.3390/ijms17050758ijms17050758Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and RatsMin Ji Bak0Van-Long Truong1Se-Yeon Ko2Xuan Ngan Giang Nguyen3Pajaree Ingkasupart4Mira Jun5Jin Young Shin6Woo-Sik Jeong7Department of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, KoreaDepartment of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, KoreaDepartment of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, KoreaDepartment of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, KoreaDepartment of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, KoreaDepartment of Food Science and Nutrition, Dong-A University, Busan 49315, KoreaChemistry and Environmental Science, City University of New York—Medgar Evers College, New York, NY 11225, USADepartment of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, KoreaIn the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of wild grape seed procyanidins (WGP) against oxidative stress damage in ethanol-treated HepG2 cell and Sprague-Dawley (SD)-rat models. In HepG2 cells, WGP not only diminished the ethanol (EtOH, 100 mM)-induced reactive oxygen species (ROS) formation and cytochrome P450 2E1 (CYP2E1) expression, but also renovated both the activity and expression of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase. Additionally, to investigate the hepatoprotective effect of WGP, rats were orally administered 10 or 50 mg/kg WGP once daily for seven days prior to the single oral administration of EtOH (6 g/kg). The results show that WGP administration decreased the EtOH-induced augment of the levels of serum aspartate transaminase and alanine transaminase as well as serum alcohol and acetaldehyde. WGP treatment upregulated the activities and protein levels of hepatic alcohol dehydrogenase, aldehyde dehydrogenase, and antioxidant enzymes but downregulated the protein expression level of liver CYP2E1 in EtOH-treated rats. Moreover, the decreased phosphorylation levels of mitogen activated protein kinases (MAPKs) by ethanol were induced in both HepG2 cell and rat models. Overall, pretreatment of WGP displayed the protective activity against EtOH-mediated toxicity through the regulation of antioxidant enzymes and alcohol metabolism systems via MAPKs pathways.http://www.mdpi.com/1422-0067/17/5/758procyanidinsalcoholoxidative stresshepatoprotectionCYP2E1MAPKs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Min Ji Bak Van-Long Truong Se-Yeon Ko Xuan Ngan Giang Nguyen Pajaree Ingkasupart Mira Jun Jin Young Shin Woo-Sik Jeong |
spellingShingle |
Min Ji Bak Van-Long Truong Se-Yeon Ko Xuan Ngan Giang Nguyen Pajaree Ingkasupart Mira Jun Jin Young Shin Woo-Sik Jeong Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats International Journal of Molecular Sciences procyanidins alcohol oxidative stress hepatoprotection CYP2E1 MAPKs |
author_facet |
Min Ji Bak Van-Long Truong Se-Yeon Ko Xuan Ngan Giang Nguyen Pajaree Ingkasupart Mira Jun Jin Young Shin Woo-Sik Jeong |
author_sort |
Min Ji Bak |
title |
Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats |
title_short |
Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats |
title_full |
Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats |
title_fullStr |
Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats |
title_full_unstemmed |
Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats |
title_sort |
antioxidant and hepatoprotective effects of procyanidins from wild grape (vitis amurensis) seeds in ethanol-induced cells and rats |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2016-05-01 |
description |
In the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of wild grape seed procyanidins (WGP) against oxidative stress damage in ethanol-treated HepG2 cell and Sprague-Dawley (SD)-rat models. In HepG2 cells, WGP not only diminished the ethanol (EtOH, 100 mM)-induced reactive oxygen species (ROS) formation and cytochrome P450 2E1 (CYP2E1) expression, but also renovated both the activity and expression of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase. Additionally, to investigate the hepatoprotective effect of WGP, rats were orally administered 10 or 50 mg/kg WGP once daily for seven days prior to the single oral administration of EtOH (6 g/kg). The results show that WGP administration decreased the EtOH-induced augment of the levels of serum aspartate transaminase and alanine transaminase as well as serum alcohol and acetaldehyde. WGP treatment upregulated the activities and protein levels of hepatic alcohol dehydrogenase, aldehyde dehydrogenase, and antioxidant enzymes but downregulated the protein expression level of liver CYP2E1 in EtOH-treated rats. Moreover, the decreased phosphorylation levels of mitogen activated protein kinases (MAPKs) by ethanol were induced in both HepG2 cell and rat models. Overall, pretreatment of WGP displayed the protective activity against EtOH-mediated toxicity through the regulation of antioxidant enzymes and alcohol metabolism systems via MAPKs pathways. |
topic |
procyanidins alcohol oxidative stress hepatoprotection CYP2E1 MAPKs |
url |
http://www.mdpi.com/1422-0067/17/5/758 |
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