Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency
In animal experiments, hippocampal neurogenesis and the activity of thiamine-dependent transketolase decrease markedly under conditions of thiamine deficiency. To further investigate the effect of thiamine deficiency on the proliferation of hippocampal progenitor cells (HPCs) and the potential mecha...
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Online Access: | http://dx.doi.org/10.1155/2014/572915 |
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doaj-e3b8c5c3b4ee4b6fbaf3dded584465432020-11-24T22:18:57ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/572915572915Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine DeficiencyYanling Zhao0Yiying Wu1Haolu Hu2Jinghui Cai3Min Ning4Xiushi Ni5Chunjiu Zhong6Department of Geriatrics, Shanghai First People’s Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, ChinaDepartment of Geriatrics, Shanghai First People’s Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, ChinaDepartment of Geriatrics, Shanghai First People’s Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, ChinaDepartment of Geriatrics, Shanghai First People’s Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, ChinaDepartment of Geriatrics, Shanghai First People’s Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, ChinaDepartment of Geriatrics, Shanghai First People’s Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, ChinaDepartment of Neurology, Zhongshan Hospital Affiliated Fudan University, 180 Fenglin Road, Shanghai 200032, ChinaIn animal experiments, hippocampal neurogenesis and the activity of thiamine-dependent transketolase decrease markedly under conditions of thiamine deficiency. To further investigate the effect of thiamine deficiency on the proliferation of hippocampal progenitor cells (HPCs) and the potential mechanisms involved in this effect, we cultured HPCs in vitro in the absence of thiamine and found that proliferation and transketolase activity were both significantly repressed. Furthermore, specific inhibition of transketolase activity by oxythiamine strongly inhibited HPC proliferation in a dose-dependent manner. However, thiamine deficiency itself inhibited the proliferation to a greater degree than did oxythiamine. Taken together, our results suggest that modulation of transketolase activity might be one of the mechanisms by which thiamine regulates the proliferation of hippocampal progenitor cells.http://dx.doi.org/10.1155/2014/572915 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanling Zhao Yiying Wu Haolu Hu Jinghui Cai Min Ning Xiushi Ni Chunjiu Zhong |
spellingShingle |
Yanling Zhao Yiying Wu Haolu Hu Jinghui Cai Min Ning Xiushi Ni Chunjiu Zhong Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency BioMed Research International |
author_facet |
Yanling Zhao Yiying Wu Haolu Hu Jinghui Cai Min Ning Xiushi Ni Chunjiu Zhong |
author_sort |
Yanling Zhao |
title |
Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency |
title_short |
Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency |
title_full |
Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency |
title_fullStr |
Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency |
title_full_unstemmed |
Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency |
title_sort |
downregulation of transketolase activity is related to inhibition of hippocampal progenitor cell proliferation induced by thiamine deficiency |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
In animal experiments, hippocampal neurogenesis and the activity of thiamine-dependent transketolase decrease markedly under conditions of thiamine deficiency. To further investigate the effect of thiamine deficiency on the proliferation of hippocampal progenitor cells (HPCs) and the potential mechanisms involved in this effect, we cultured HPCs in vitro in the absence of thiamine and found that proliferation and transketolase activity were both significantly repressed. Furthermore, specific inhibition of transketolase activity by oxythiamine strongly inhibited HPC proliferation in a dose-dependent manner. However, thiamine deficiency itself inhibited the proliferation to a greater degree than did oxythiamine. Taken together, our results suggest that modulation of transketolase activity might be one of the mechanisms by which thiamine regulates the proliferation of hippocampal progenitor cells. |
url |
http://dx.doi.org/10.1155/2014/572915 |
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