Inhibition effects of Bevacizumab on migration of human Tenon capsule fibroblasts

• Main Authors: Jing Li, An-Ming Xie
• Format: Article
• Language: English
• Published: Press of International Journal of Ophthalmology (IJO PRESS) 2015-01-01
• Series: Guoji Yanke Zazhi
• Subjects: human Tenon capsule fibroblasts; bevacizumab; migration; bleb scarring
• Online Access: http://ies.ijo.cn/cn_publish/2015/1/201501008.pdf

AIM: To investigate the inhibition effect of bevacizumab on human Tenon capsule fibroblasts(HTFs)and discuss the countermeasures of bleb scarringscarring in glaucoma surgery countermeasures. <p>METHODS: Adopted cell recovery method and followed the aseptic principles, we performed the culture of HTFs which came from the Central Laboratory of Shaanxi People's Hospital cell library. Wound Healing assay: We scraped a cell-free zone on the cell surface when the cells reached confluence at 80%. The control group was added to serum-free DMEM medium. The HTFs of bevacizumab group were stimulated with 1mg/mL concentrations without DEME for 0, 24, 48, and 72h. The scratch width was observed and measured.<p>RESULTS:HTFs were long fusiform shape under microscope, the nucleus is in the center of the cell with larger nucleus, abundant cytoplasm, were arranged in a whorled growth out of shape, strong ability to proliferate, conform to general forms of fibroblast. The morphological and biological characteristics of cells after cryopreservation and resuscitation remain unchanged. Wound healing assay: 0h, equal to the initial width of the two groups, 24h when the migration distance of the two groups of cells are basically the same, 48h when the control group cell migration distance is greater than that in the bevacizumab processing group, 72h when the control group scratches basichealing, bevacizumab treated cells migrate closer than 48h no significant change, and a lot of cells died. <p>CONCLUSION:Cell recovery method can successfully cultured HTFs, which was stability on morphology and biological properties, laying the cellular basis for experimental research.Fibroblast itself has a strong ability to migrate, outside-derived bevacizumab can inhibit HTFs migration evidently and it will cause excessive cell death when. Bevacizumab has certain extent inhibitory effect on HTFs migration, and it is likely to become one of the important drugs for creating bleb scarring after glaucoma surgery in the future.