Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation
AIM: To investigate the effect of endothelial progenitor cells (EPCs) labeled by carboxy fluorescein diacetate succinimidyl ester (CFSE) on murine oxygen-induced retinopathy (OIR) by intravitreal transplantation. METHODS: After isolated from human umbilical cord blood mononuclear cells, EPCs were c...
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doaj-e3cdac3dab6f4168b9d0948ace36dee52020-11-24T22:09:15ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982016-11-019111578158310.18240/ijo.2016.11.07Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantationDan Wang0Bo Zhang1Hui Shi2Wei Yang3Ming-Chao Bi4Xiang-Fu Song5Chen Zhang6Jian-Hui Cheng7Ji-Long Hao8E Song9Department of Ophthalmology, First Hospital, Jilin University, Changchun 130021, Jilin Province, ChinaDepartment of Neurosurgery, First Hospital, Jilin University, Changchun 130021, Jilin Province, ChinaDepartment of Ophthalmology, First Hospital, Jilin University, Changchun 130021, Jilin Province, ChinaDepartment of Ophthalmology, First Hospital, Jilin University, Changchun 130021, Jilin Province, ChinaDepartment of Ophthalmology, First Hospital, Jilin University, Changchun 130021, Jilin Province, ChinaSchool of Public Health, Jilin University, Changchun 130021, Jilin Province, ChinaSchool of Medicine, Tongji University, Shanghai 200092, ChinaLixiang Eye Hospital, Soochow University, Soochow 215021, Jiangsu Province, ChinaDepartment of Ophthalmology, First Hospital, Jilin University, Changchun 130021, Jilin Province, ChinaDepartment of Ophthalmology, First Hospital, Jilin University, Changchun 130021, Jilin Province, China; Lixiang Eye Hospital, Soochow University, Soochow 215021, Jiangsu Province, ChinaAIM: To investigate the effect of endothelial progenitor cells (EPCs) labeled by carboxy fluorescein diacetate succinimidyl ester (CFSE) on murine oxygen-induced retinopathy (OIR) by intravitreal transplantation. METHODS: After isolated from human umbilical cord blood mononuclear cells, EPCs were cultivated and then labeled with CFSE in vitro. C57BL/6J mice were placed to 75% hyperoxia chamber from P7 to P12 to establish OIR model. At P12, OIR mice were intravitreally injected with 1 μL suspension contained 2×105 EPCs (EPCs group) or isometric phosphate buffered saline (PBS group). The contralateral eye of each mice received no injection (OIR group). Evans blue angiography and frozen section were examined to track the labeled cells in OIR group at P15 and P19. Using retina paraffin sections and adenosinediphos phatase staining at P12 and P19, the effect of EPCs on OIR mice was evaluated quantitatively and qualitatively. RESULTS: The retinas from EPCs group with less non-perfusion area and fewer peripheral tufts were observed at P19, comparing with that from PBS or OIR group. The retinopathy in EPCs group receded earlier with less non-ganglion cells and neovascular nuclei, together with relatively regular distribution. The counts of the neovascular nuclei at P19 were reduced by 44% or 45%, compared with those of OIR group or PBS group respectively. Three days after EPCs injection, a large number of EPCs appeared in the vitreous cavity and adhered to the retinal surface. While at one week, the cells gathered between the internal plexiform layer and the inner limiting membrane, and some EPCs appeared in retinal vessels. CONCLUSION: EPCs transplantation can participate in the reparative procedure of the neovascularization in OIR.http://www.ijo.cn/en_publish/2016/11/20161107.pdfendothelial progenitor cellsretinal neovascularizationoxygen induced retinopathyretinopathy of prematuritycell transplantationmousemouse |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dan Wang Bo Zhang Hui Shi Wei Yang Ming-Chao Bi Xiang-Fu Song Chen Zhang Jian-Hui Cheng Ji-Long Hao E Song |
spellingShingle |
Dan Wang Bo Zhang Hui Shi Wei Yang Ming-Chao Bi Xiang-Fu Song Chen Zhang Jian-Hui Cheng Ji-Long Hao E Song Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation International Journal of Ophthalmology endothelial progenitor cells retinal neovascularization oxygen induced retinopathy retinopathy of prematurity cell transplantation mouse mouse |
author_facet |
Dan Wang Bo Zhang Hui Shi Wei Yang Ming-Chao Bi Xiang-Fu Song Chen Zhang Jian-Hui Cheng Ji-Long Hao E Song |
author_sort |
Dan Wang |
title |
Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation |
title_short |
Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation |
title_full |
Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation |
title_fullStr |
Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation |
title_full_unstemmed |
Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation |
title_sort |
effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
International Journal of Ophthalmology |
issn |
2222-3959 2227-4898 |
publishDate |
2016-11-01 |
description |
AIM: To investigate the effect of endothelial progenitor cells (EPCs) labeled by carboxy fluorescein diacetate succinimidyl ester (CFSE) on murine oxygen-induced retinopathy (OIR) by intravitreal transplantation.
METHODS: After isolated from human umbilical cord blood mononuclear cells, EPCs were cultivated and then labeled with CFSE in vitro. C57BL/6J mice were placed to 75% hyperoxia chamber from P7 to P12 to establish OIR model. At P12, OIR mice were intravitreally injected with 1 μL suspension contained 2×105 EPCs (EPCs group) or isometric phosphate buffered saline (PBS group). The contralateral eye of each mice received no injection (OIR group). Evans blue angiography and frozen section were examined to track the labeled cells in OIR group at P15 and P19. Using retina paraffin sections and adenosinediphos phatase staining at P12 and P19, the effect of EPCs on OIR mice was evaluated quantitatively and qualitatively.
RESULTS: The retinas from EPCs group with less non-perfusion area and fewer peripheral tufts were observed at P19, comparing with that from PBS or OIR group. The retinopathy in EPCs group receded earlier with less non-ganglion cells and neovascular nuclei, together with relatively regular distribution. The counts of the neovascular nuclei at P19 were reduced by 44% or 45%, compared with those of OIR group or PBS group respectively. Three days after EPCs injection, a large number of EPCs appeared in the vitreous cavity and adhered to the retinal surface. While at one week, the cells gathered between the internal plexiform layer and the inner limiting membrane, and some EPCs appeared in retinal vessels.
CONCLUSION: EPCs transplantation can participate in the reparative procedure of the neovascularization in OIR. |
topic |
endothelial progenitor cells retinal neovascularization oxygen induced retinopathy retinopathy of prematurity cell transplantation mouse mouse |
url |
http://www.ijo.cn/en_publish/2016/11/20161107.pdf |
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