Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål)

• Main Authors: Guangcun He, Yi Ding, Lili Zhu, Xinxin Peng, Shuzhen Wang, Bingfang Liu, Chunxiao Zhang, Hangjin Yu, Xi Zhou, Shengli Jing
• Format: Article
• Language: English
• Published: MDPI AG 2012-07-01
• Series: International Journal of Molecular Sciences
• Subjects: <em>Nilaparvata lugens</em> microsatellites; polymorphism; genetic diversity
• Online Access: http://www.mdpi.com/1422-0067/13/8/9527
• ISSN: 1422-0067

Brown planthopper (<em>Nilaparvata lugens</em> Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from <em>N. lugens</em> genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics.