Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål)
Brown planthopper (<em>Nilaparvata lugens</em> Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from <em>N. lugens</em> genomic libraries using the met...
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MDPI AG
2012-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/13/8/9527 |
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doaj-e3dae61e7b5049d6a8f251ab1a4698612020-11-25T00:13:10ZengMDPI AGInternational Journal of Molecular Sciences1422-00672012-07-011389527953310.3390/ijms13089527Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål)Guangcun HeYi DingLili ZhuXinxin PengShuzhen WangBingfang LiuChunxiao ZhangHangjin YuXi ZhouShengli JingBrown planthopper (<em>Nilaparvata lugens</em> Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from <em>N. lugens</em> genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics.http://www.mdpi.com/1422-0067/13/8/9527<em>Nilaparvata lugens</em>microsatellitespolymorphismgenetic diversity |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Guangcun He Yi Ding Lili Zhu Xinxin Peng Shuzhen Wang Bingfang Liu Chunxiao Zhang Hangjin Yu Xi Zhou Shengli Jing |
| spellingShingle |
Guangcun He Yi Ding Lili Zhu Xinxin Peng Shuzhen Wang Bingfang Liu Chunxiao Zhang Hangjin Yu Xi Zhou Shengli Jing Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål) International Journal of Molecular Sciences <em>Nilaparvata lugens</em> microsatellites polymorphism genetic diversity |
| author_facet |
Guangcun He Yi Ding Lili Zhu Xinxin Peng Shuzhen Wang Bingfang Liu Chunxiao Zhang Hangjin Yu Xi Zhou Shengli Jing |
| author_sort |
Guangcun He |
| title |
Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål) |
| title_short |
Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål) |
| title_full |
Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål) |
| title_fullStr |
Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål) |
| title_full_unstemmed |
Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål) |
| title_sort |
isolation and characterization of microsatellite markers in brown planthopper (<em>nilaparvata </em><em>l</em><em>ugens</em> stål) |
| publisher |
MDPI AG |
| series |
International Journal of Molecular Sciences |
| issn |
1422-0067 |
| publishDate |
2012-07-01 |
| description |
Brown planthopper (<em>Nilaparvata lugens</em> Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from <em>N. lugens</em> genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics. |
| topic |
<em>Nilaparvata lugens</em> microsatellites polymorphism genetic diversity |
| url |
http://www.mdpi.com/1422-0067/13/8/9527 |
| work_keys_str_mv |
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