A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting
She-donkey’s milk (DM) and goat’s milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess...
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2014-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/15/8/13697 |
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doaj-e3e397472e144d36bbc3ae495c1646d22020-11-24T23:55:27ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-08-01158136971371910.3390/ijms150813697ijms150813697A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS FingerprintingFrancesco Di Girolamo0Andrea Masotti1Guglielmo Salvatori2Margherita Scapaticci3Maurizio Muraca4Lorenza Putignani5Department of Laboratory Medicine, Bambino Gesù Children's Hospital, IRCCS,Piazza Sant'Onofrio 4, Rome 00165, ItalyGene Expression-Microarrays Laboratory, Bambino Gesù Children's Hospital, IRCCS,Piazza Sant'Onofrio 4, Rome 00165, ItalyNeonatal Intensive Care Unit, Department of Medical and Surgical Neonatology,Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4, Rome 00165, ItalyDepartment of Laboratory Medicine, San Camillo Hospital, Viale Vittorio Veneto 18,Treviso 31100, ItalyDepartment of Laboratory Medicine, Bambino Gesù Children's Hospital, IRCCS,Piazza Sant'Onofrio 4, Rome 00165, ItalyParasitology Unit, Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4,Rome 00165, ItalyShe-donkey’s milk (DM) and goat’s milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess the authenticity of these precious nutrients. In this experimental work, a sensitive and robust matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling was designed to assess the genuineness of DM and GM milks. This workflow allows the identification of DM and GM adulteration at levels of 0.5%, thus, representing a sensitive tool for milk adulteration analysis, if compared with other laborious and time-consuming analytical procedures.http://www.mdpi.com/1422-0067/15/8/13697milkadulterationMALDI-TOF MS |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Francesco Di Girolamo Andrea Masotti Guglielmo Salvatori Margherita Scapaticci Maurizio Muraca Lorenza Putignani |
| spellingShingle |
Francesco Di Girolamo Andrea Masotti Guglielmo Salvatori Margherita Scapaticci Maurizio Muraca Lorenza Putignani A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting International Journal of Molecular Sciences milk adulteration MALDI-TOF MS |
| author_facet |
Francesco Di Girolamo Andrea Masotti Guglielmo Salvatori Margherita Scapaticci Maurizio Muraca Lorenza Putignani |
| author_sort |
Francesco Di Girolamo |
| title |
A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting |
| title_short |
A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting |
| title_full |
A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting |
| title_fullStr |
A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting |
| title_full_unstemmed |
A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting |
| title_sort |
sensitive and effective proteomic approach to identify she-donkey’s and goat’s milk adulterations by maldi-tof ms fingerprinting |
| publisher |
MDPI AG |
| series |
International Journal of Molecular Sciences |
| issn |
1422-0067 |
| publishDate |
2014-08-01 |
| description |
She-donkey’s milk (DM) and goat’s milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess the authenticity of these precious nutrients. In this experimental work, a sensitive and robust matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling was designed to assess the genuineness of DM and GM milks. This workflow allows the identification of DM and GM adulteration at levels of 0.5%, thus, representing a sensitive tool for milk adulteration analysis, if compared with other laborious and time-consuming analytical procedures. |
| topic |
milk adulteration MALDI-TOF MS |
| url |
http://www.mdpi.com/1422-0067/15/8/13697 |
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