Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by <i>Leptospira</i> in TLR2-Overexpressed HEK293 Cells
<i>Leptospira</i> infection can cause potential hazards to human health by stimulating inflammation, which is mediated mainly through the Toll-like receptor 2 (TLR2) pathway. Gold nanoparticles (AuNPs) are promising for medical applications, as they display both bioinert and noncytotoxic...
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doaj-e3e537a4c9c7493fbcbe1cbb191e47c82020-12-17T00:00:26ZengMDPI AGNanomaterials2079-49912020-12-01102522252210.3390/nano10122522Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by <i>Leptospira</i> in TLR2-Overexpressed HEK293 CellsKanidta Sooklert0Chawikan Boonwong1Pattama Ekpo2Rojrit Rojanathanes3Kanitha Patarakul4Chintana Chirathaworn5Sasin Thamakaison6Amornpun Sereemaspun7Nanomedicine Research Unit, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandResearch Division, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandResearch Division, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandNanomedicine Research Unit, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandNanomedicine Research Unit, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand<i>Leptospira</i> infection can cause potential hazards to human health by stimulating inflammation, which is mediated mainly through the Toll-like receptor 2 (TLR2) pathway. Gold nanoparticles (AuNPs) are promising for medical applications, as they display both bioinert and noncytotoxic characteristics. AuNPs have been shown to have the ability to modify immune responses. To understand the in vitro immunomodulatory effect of AuNPs in a <i>Leptospira</i> infection model, the activation of TLR2 expression was examined in HEK-Blue-hTLR2 cells treated with <i>Leptospira</i> serovars and/or AuNPs (10 and 20 nm). The ability of AuNPs to modulate an inflammatory response induced by <i>Leptospira</i> was examined in terms of transcript expression level modulation of three proinflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-1β and IL-6) using two-stage quantitative real-time reverse transcriptase PCR. The results revealed that the administration of 10 nm AuNPs could augment the <i>Leptospira</i>-induced TLR2 signaling response and upregulate the expression of all three cytokine gene transcripts, whereas the 20 nm AuNPs attenuated the TLR2 activation and expression of proinflammatory cytokines. This indicates that AuNPs can modulate inflammatory parameters in <i>Leptospira</i> infection and different-sized AuNPs had different immunomodulatory functions in this model.https://www.mdpi.com/2079-4991/10/12/2522gold nanoparticleTLR2leptospirosissize-dependent |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kanidta Sooklert Chawikan Boonwong Pattama Ekpo Rojrit Rojanathanes Kanitha Patarakul Chintana Chirathaworn Sasin Thamakaison Amornpun Sereemaspun |
spellingShingle |
Kanidta Sooklert Chawikan Boonwong Pattama Ekpo Rojrit Rojanathanes Kanitha Patarakul Chintana Chirathaworn Sasin Thamakaison Amornpun Sereemaspun Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by <i>Leptospira</i> in TLR2-Overexpressed HEK293 Cells Nanomaterials gold nanoparticle TLR2 leptospirosis size-dependent |
author_facet |
Kanidta Sooklert Chawikan Boonwong Pattama Ekpo Rojrit Rojanathanes Kanitha Patarakul Chintana Chirathaworn Sasin Thamakaison Amornpun Sereemaspun |
author_sort |
Kanidta Sooklert |
title |
Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by <i>Leptospira</i> in TLR2-Overexpressed HEK293 Cells |
title_short |
Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by <i>Leptospira</i> in TLR2-Overexpressed HEK293 Cells |
title_full |
Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by <i>Leptospira</i> in TLR2-Overexpressed HEK293 Cells |
title_fullStr |
Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by <i>Leptospira</i> in TLR2-Overexpressed HEK293 Cells |
title_full_unstemmed |
Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by <i>Leptospira</i> in TLR2-Overexpressed HEK293 Cells |
title_sort |
effect of gold nanoparticles on the tlr2-mediated inflammatory responses induced by <i>leptospira</i> in tlr2-overexpressed hek293 cells |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2020-12-01 |
description |
<i>Leptospira</i> infection can cause potential hazards to human health by stimulating inflammation, which is mediated mainly through the Toll-like receptor 2 (TLR2) pathway. Gold nanoparticles (AuNPs) are promising for medical applications, as they display both bioinert and noncytotoxic characteristics. AuNPs have been shown to have the ability to modify immune responses. To understand the in vitro immunomodulatory effect of AuNPs in a <i>Leptospira</i> infection model, the activation of TLR2 expression was examined in HEK-Blue-hTLR2 cells treated with <i>Leptospira</i> serovars and/or AuNPs (10 and 20 nm). The ability of AuNPs to modulate an inflammatory response induced by <i>Leptospira</i> was examined in terms of transcript expression level modulation of three proinflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-1β and IL-6) using two-stage quantitative real-time reverse transcriptase PCR. The results revealed that the administration of 10 nm AuNPs could augment the <i>Leptospira</i>-induced TLR2 signaling response and upregulate the expression of all three cytokine gene transcripts, whereas the 20 nm AuNPs attenuated the TLR2 activation and expression of proinflammatory cytokines. This indicates that AuNPs can modulate inflammatory parameters in <i>Leptospira</i> infection and different-sized AuNPs had different immunomodulatory functions in this model. |
topic |
gold nanoparticle TLR2 leptospirosis size-dependent |
url |
https://www.mdpi.com/2079-4991/10/12/2522 |
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