Molecular Imaging of Pancreatic Duct Adenocarcinoma Using a Type 2 Cannabinoid Receptor-Targeted Near-Infrared Fluorescent Probe

Molecular Imaging of Pancreatic Duct Adenocarcinoma Using a Type 2 Cannabinoid Receptor-Targeted Near-Infrared Fluorescent Probe

Imaging probes targeting type 2 cannabinoid receptor (CB2R) overexpressed in pancreatic duct adenocarcinoma (PDAC) tissue have the potential to improve early detection and surgical outcome of PDAC. The aim of our study was to evaluate the molecular imaging potential of a CB2R-targeted near-infrared...

Full description

Bibliographic Details
Main Authors: Xiaoxia Guo, Xiaoxi Ling, Fang Du, Qingbing Wang, Wei Huang, Zhongmin Wang, Xiaoyi Ding, Mingfeng Bai, Zhiyuan Wu
Format: Article
Language:English
Published: Elsevier 2018-10-01
Series:Translational Oncology
Online Access:http://www.sciencedirect.com/science/article/pii/S1936523318301827
id doaj-e3e711fa859f4236b81c746a233bf3be
record_format Article
spelling doaj-e3e711fa859f4236b81c746a233bf3be2020-11-24T23:31:19ZengElsevierTranslational Oncology1936-52332018-10-0111510651073Molecular Imaging of Pancreatic Duct Adenocarcinoma Using a Type 2 Cannabinoid Receptor-Targeted Near-Infrared Fluorescent ProbeXiaoxia Guo0Xiaoxi Ling1Fang Du2Qingbing Wang3Wei Huang4Zhongmin Wang5Xiaoyi Ding6Mingfeng Bai7Zhiyuan Wu8Department of Interventional Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDepartment of Medicine, University of Pittsburgh, 3501 Fifth Ave, Pittsburgh, PA 15213, USADepartment of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDepartment of Interventional Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDepartment of Interventional Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDepartment of Interventional Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDepartment of Interventional Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Address all correspondence to: Xiaoyi Ding or Zhiyuan Wu, Department of Interventional Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China or Mingfeng Bai, Vanderbilt University Institute of Imaging Science (VUIIS), Vanderbilt University Medical Center, Nashville, TN, 37232, USA.Vanderbilt University Institute of Imaging Science (VUIIS), Vanderbilt University Medical Center, Nashville, TN 37232, USA; Center for Molecular Probes, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Radiology and Radiological Sciences, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Vanderbilt-Ingram Cancer Center (VICC), Vanderbilt University Medical Center, Nashville, TN 37232, USA; Address all correspondence to: Xiaoyi Ding or Zhiyuan Wu, Department of Interventional Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China or Mingfeng Bai, Vanderbilt University Institute of Imaging Science (VUIIS), Vanderbilt University Medical Center, Nashville, TN, 37232, USA.Department of Interventional Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Address all correspondence to: Xiaoyi Ding or Zhiyuan Wu, Department of Interventional Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China or Mingfeng Bai, Vanderbilt University Institute of Imaging Science (VUIIS), Vanderbilt University Medical Center, Nashville, TN, 37232, USA.Imaging probes targeting type 2 cannabinoid receptor (CB2R) overexpressed in pancreatic duct adenocarcinoma (PDAC) tissue have the potential to improve early detection and surgical outcome of PDAC. The aim of our study was to evaluate the molecular imaging potential of a CB2R-targeted near-infrared (NIR) fluorescent probe (NIR760-XLP6) for PDAC. CB2R overexpression was observed in both PDAC patient tissues and various pancreatic cancer cell lines. In vitro fluorescence imaging indicated specific binding of NIR760-XLP6 to CB2R in human PDAC PANC-1 cells. In a xenograft mouse tumor model, NIR760-XLP6 showed remarkable 50- (ex vivo) and 3.2-fold (in vivo) tumor to normal contrast enhancement with minimal liver and kidney uptake. In a PDAC lymph node metastasis model, significant signal contrast was observed in bilateral axillary lymph nodes with PDAC metastasis after injection of the probe. In conclusion, NIR760-XLP6 exhibits promising characteristics for imaging PDAC, and CB2R appears to be an attractive target for PDAC imaging.http://www.sciencedirect.com/science/article/pii/S1936523318301827
collection DOAJ
language English
format Article
sources DOAJ
author Xiaoxia Guo
Xiaoxi Ling
Fang Du
Qingbing Wang
Wei Huang
Zhongmin Wang
Xiaoyi Ding
Mingfeng Bai
Zhiyuan Wu
spellingShingle Xiaoxia Guo
Xiaoxi Ling
Fang Du
Qingbing Wang
Wei Huang
Zhongmin Wang
Xiaoyi Ding
Mingfeng Bai
Zhiyuan Wu
Molecular Imaging of Pancreatic Duct Adenocarcinoma Using a Type 2 Cannabinoid Receptor-Targeted Near-Infrared Fluorescent Probe
Translational Oncology
author_facet Xiaoxia Guo
Xiaoxi Ling
Fang Du
Qingbing Wang
Wei Huang
Zhongmin Wang
Xiaoyi Ding
Mingfeng Bai
Zhiyuan Wu
author_sort Xiaoxia Guo
title Molecular Imaging of Pancreatic Duct Adenocarcinoma Using a Type 2 Cannabinoid Receptor-Targeted Near-Infrared Fluorescent Probe
title_short Molecular Imaging of Pancreatic Duct Adenocarcinoma Using a Type 2 Cannabinoid Receptor-Targeted Near-Infrared Fluorescent Probe
title_full Molecular Imaging of Pancreatic Duct Adenocarcinoma Using a Type 2 Cannabinoid Receptor-Targeted Near-Infrared Fluorescent Probe
title_fullStr Molecular Imaging of Pancreatic Duct Adenocarcinoma Using a Type 2 Cannabinoid Receptor-Targeted Near-Infrared Fluorescent Probe
title_full_unstemmed Molecular Imaging of Pancreatic Duct Adenocarcinoma Using a Type 2 Cannabinoid Receptor-Targeted Near-Infrared Fluorescent Probe
title_sort molecular imaging of pancreatic duct adenocarcinoma using a type 2 cannabinoid receptor-targeted near-infrared fluorescent probe
publisher Elsevier
series Translational Oncology
issn 1936-5233
publishDate 2018-10-01
description Imaging probes targeting type 2 cannabinoid receptor (CB2R) overexpressed in pancreatic duct adenocarcinoma (PDAC) tissue have the potential to improve early detection and surgical outcome of PDAC. The aim of our study was to evaluate the molecular imaging potential of a CB2R-targeted near-infrared (NIR) fluorescent probe (NIR760-XLP6) for PDAC. CB2R overexpression was observed in both PDAC patient tissues and various pancreatic cancer cell lines. In vitro fluorescence imaging indicated specific binding of NIR760-XLP6 to CB2R in human PDAC PANC-1 cells. In a xenograft mouse tumor model, NIR760-XLP6 showed remarkable 50- (ex vivo) and 3.2-fold (in vivo) tumor to normal contrast enhancement with minimal liver and kidney uptake. In a PDAC lymph node metastasis model, significant signal contrast was observed in bilateral axillary lymph nodes with PDAC metastasis after injection of the probe. In conclusion, NIR760-XLP6 exhibits promising characteristics for imaging PDAC, and CB2R appears to be an attractive target for PDAC imaging.
url http://www.sciencedirect.com/science/article/pii/S1936523318301827
work_keys_str_mv AT xiaoxiaguo molecularimagingofpancreaticductadenocarcinomausingatype2cannabinoidreceptortargetednearinfraredfluorescentprobe
AT xiaoxiling molecularimagingofpancreaticductadenocarcinomausingatype2cannabinoidreceptortargetednearinfraredfluorescentprobe
AT fangdu molecularimagingofpancreaticductadenocarcinomausingatype2cannabinoidreceptortargetednearinfraredfluorescentprobe
AT qingbingwang molecularimagingofpancreaticductadenocarcinomausingatype2cannabinoidreceptortargetednearinfraredfluorescentprobe
AT weihuang molecularimagingofpancreaticductadenocarcinomausingatype2cannabinoidreceptortargetednearinfraredfluorescentprobe
AT zhongminwang molecularimagingofpancreaticductadenocarcinomausingatype2cannabinoidreceptortargetednearinfraredfluorescentprobe
AT xiaoyiding molecularimagingofpancreaticductadenocarcinomausingatype2cannabinoidreceptortargetednearinfraredfluorescentprobe
AT mingfengbai molecularimagingofpancreaticductadenocarcinomausingatype2cannabinoidreceptortargetednearinfraredfluorescentprobe
AT zhiyuanwu molecularimagingofpancreaticductadenocarcinomausingatype2cannabinoidreceptortargetednearinfraredfluorescentprobe
_version_ 1725538386809192448

Similar Items