IGF-1 Upregulates Biglycan and Decorin by Increasing Translation and Reducing ADAMTS5 Expression
Proteoglycan (PG) is a glycosaminoglycan (GAG)-conjugated protein essential for maintaining tissue strength and elasticity. The most abundant skin PGs, biglycan and decorin, have been reported to decrease as skin ages. Insulin-like growth factor-1 (IGF-1) is important in various physiological functi...
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doaj-e3ea07e68cfd4bfc91fa9030c7205be92021-01-31T00:04:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01221403140310.3390/ijms22031403IGF-1 Upregulates Biglycan and Decorin by Increasing Translation and Reducing ADAMTS5 ExpressionHanon Lee0Jiyeong Lim1Jang-Hee Oh2Soyun Cho3Jin Ho Chung4Department of Biomedical Sciences, Seoul National University Graduate School, Seoul 03080, KoreaDepartment of Biomedical Sciences, Seoul National University Graduate School, Seoul 03080, KoreaDepartment of Dermatology, Seoul National University College of Medicine, Seoul 03080, KoreaDepartment of Dermatology, Seoul National University College of Medicine, Seoul 03080, KoreaDepartment of Biomedical Sciences, Seoul National University Graduate School, Seoul 03080, KoreaProteoglycan (PG) is a glycosaminoglycan (GAG)-conjugated protein essential for maintaining tissue strength and elasticity. The most abundant skin PGs, biglycan and decorin, have been reported to decrease as skin ages. Insulin-like growth factor-1 (IGF-1) is important in various physiological functions such as cell survival, growth, and apoptosis. It is well known that the serum level of IGF-1 decreases with age. Therefore, we investigated whether and how IGF-1 affects biglycan and decorin. When primary cultured normal human dermal fibroblasts (NHDFs) were treated with IGF-1, protein levels of biglycan and decorin increased, despite no difference in mRNA expression. This increase was not inhibited by transcription blockade using actinomycin D, suggesting that it is mediated by IGF-1-induced enhanced translation. Additionally, both mRNA and protein expression of ADAMTS5, a PG-degrading enzyme, were decreased in IGF-1-treated NHDFs. Knockdown of ADAMTS5 via RNA interference increased protein expression of biglycan and decorin. Moreover, mRNA and protein expression of ADAMTS5 increased in aged human skin tissues compared to young tissue. Overall, IGF-1 increases biglycan and decorin, which is achieved by improving protein translation to increase synthesis and preventing ADAMTS5-mediated degradation. This suggests a new role of IGF-1 as a regulator for biglycan and decorin in skin aging process.https://www.mdpi.com/1422-0067/22/3/1403insulin-like growth factor-1 (IGF-1)proteoglycan (PG)biglycandecorina disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5)skin aging |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hanon Lee Jiyeong Lim Jang-Hee Oh Soyun Cho Jin Ho Chung |
spellingShingle |
Hanon Lee Jiyeong Lim Jang-Hee Oh Soyun Cho Jin Ho Chung IGF-1 Upregulates Biglycan and Decorin by Increasing Translation and Reducing ADAMTS5 Expression International Journal of Molecular Sciences insulin-like growth factor-1 (IGF-1) proteoglycan (PG) biglycan decorin a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) skin aging |
author_facet |
Hanon Lee Jiyeong Lim Jang-Hee Oh Soyun Cho Jin Ho Chung |
author_sort |
Hanon Lee |
title |
IGF-1 Upregulates Biglycan and Decorin by Increasing Translation and Reducing ADAMTS5 Expression |
title_short |
IGF-1 Upregulates Biglycan and Decorin by Increasing Translation and Reducing ADAMTS5 Expression |
title_full |
IGF-1 Upregulates Biglycan and Decorin by Increasing Translation and Reducing ADAMTS5 Expression |
title_fullStr |
IGF-1 Upregulates Biglycan and Decorin by Increasing Translation and Reducing ADAMTS5 Expression |
title_full_unstemmed |
IGF-1 Upregulates Biglycan and Decorin by Increasing Translation and Reducing ADAMTS5 Expression |
title_sort |
igf-1 upregulates biglycan and decorin by increasing translation and reducing adamts5 expression |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-01-01 |
description |
Proteoglycan (PG) is a glycosaminoglycan (GAG)-conjugated protein essential for maintaining tissue strength and elasticity. The most abundant skin PGs, biglycan and decorin, have been reported to decrease as skin ages. Insulin-like growth factor-1 (IGF-1) is important in various physiological functions such as cell survival, growth, and apoptosis. It is well known that the serum level of IGF-1 decreases with age. Therefore, we investigated whether and how IGF-1 affects biglycan and decorin. When primary cultured normal human dermal fibroblasts (NHDFs) were treated with IGF-1, protein levels of biglycan and decorin increased, despite no difference in mRNA expression. This increase was not inhibited by transcription blockade using actinomycin D, suggesting that it is mediated by IGF-1-induced enhanced translation. Additionally, both mRNA and protein expression of ADAMTS5, a PG-degrading enzyme, were decreased in IGF-1-treated NHDFs. Knockdown of ADAMTS5 via RNA interference increased protein expression of biglycan and decorin. Moreover, mRNA and protein expression of ADAMTS5 increased in aged human skin tissues compared to young tissue. Overall, IGF-1 increases biglycan and decorin, which is achieved by improving protein translation to increase synthesis and preventing ADAMTS5-mediated degradation. This suggests a new role of IGF-1 as a regulator for biglycan and decorin in skin aging process. |
topic |
insulin-like growth factor-1 (IGF-1) proteoglycan (PG) biglycan decorin a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) skin aging |
url |
https://www.mdpi.com/1422-0067/22/3/1403 |
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