Metabolism of palmitic acid in the subcellular fractions of mouse brain
After an intracerebral injection of [14C]palmitic acid to C57BL/10J mice, the radioactivity in the brains decreased rapidly with time. The incorporated radioactivity was primarily in the 16:0 acyl groups of the diacyl phosphoglycerides at 1 and 3 days after injection. At longer times, increasing pro...
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doaj-e3ee502b0dd143c18bd39e2ca2a41a822021-04-24T05:49:41ZengElsevierJournal of Lipid Research0022-22751973-03-01142206214Metabolism of palmitic acid in the subcellular fractions of mouse brainGrace Y. Sun0Lloyd A. Horrocks1Laboratory of Neurochemistry, Cleveland Psychiatric Institute, Cleveland Ohio 44109, and; Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio 43210Laboratory of Neurochemistry, Cleveland Psychiatric Institute, Cleveland Ohio 44109, and; Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio 43210After an intracerebral injection of [14C]palmitic acid to C57BL/10J mice, the radioactivity in the brains decreased rapidly with time. The incorporated radioactivity was primarily in the 16:0 acyl groups of the diacyl phosphoglycerides at 1 and 3 days after injection. At longer times, increasing proportions of the radioactivity were found in cerebrosides, alkenyl groups, and other acyl groups. The specific radioactivities of the phosphoglycerides were highest in the microsomal fraction at 1 day after injection. The exchange of the diacyl glycerophosphorylcholines and diacyl glycerophosphorylethanolamines between the microsomes and the myelin required 8–14 days. When calculated on the basis of the radioactivity in the 16:0 acyl groups, the half-lives for both of these phosphoglycerides were 6–8 days in all subcellular fractions during the period from 14 to 30 days after injection. The radioactivity in the total lipids from the purified myelin fraction did not decline until more than 14 days after injection because of the reutilization of labeled 16:0 acyl groups for lipid biosynthesis. Recycling of the acyl groups explains the long halflives reported for myelin phosphoglycerides after injection of [14C]acetic acid. Lipids with a relatively high specific radioactivity were lost from the myelin fraction during the purification procedure. The most likely source of these lipids is the most recently formed myelin that is not consolidated into the myelin sheath.http://www.sciencedirect.com/science/article/pii/S002222752036908Xphosphoglyceridesturnoveracyl groupsmicrosomesmyelin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Grace Y. Sun Lloyd A. Horrocks |
spellingShingle |
Grace Y. Sun Lloyd A. Horrocks Metabolism of palmitic acid in the subcellular fractions of mouse brain Journal of Lipid Research phosphoglycerides turnover acyl groups microsomes myelin |
author_facet |
Grace Y. Sun Lloyd A. Horrocks |
author_sort |
Grace Y. Sun |
title |
Metabolism of palmitic acid in the subcellular fractions of mouse brain |
title_short |
Metabolism of palmitic acid in the subcellular fractions of mouse brain |
title_full |
Metabolism of palmitic acid in the subcellular fractions of mouse brain |
title_fullStr |
Metabolism of palmitic acid in the subcellular fractions of mouse brain |
title_full_unstemmed |
Metabolism of palmitic acid in the subcellular fractions of mouse brain |
title_sort |
metabolism of palmitic acid in the subcellular fractions of mouse brain |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1973-03-01 |
description |
After an intracerebral injection of [14C]palmitic acid to C57BL/10J mice, the radioactivity in the brains decreased rapidly with time. The incorporated radioactivity was primarily in the 16:0 acyl groups of the diacyl phosphoglycerides at 1 and 3 days after injection. At longer times, increasing proportions of the radioactivity were found in cerebrosides, alkenyl groups, and other acyl groups. The specific radioactivities of the phosphoglycerides were highest in the microsomal fraction at 1 day after injection. The exchange of the diacyl glycerophosphorylcholines and diacyl glycerophosphorylethanolamines between the microsomes and the myelin required 8–14 days. When calculated on the basis of the radioactivity in the 16:0 acyl groups, the half-lives for both of these phosphoglycerides were 6–8 days in all subcellular fractions during the period from 14 to 30 days after injection. The radioactivity in the total lipids from the purified myelin fraction did not decline until more than 14 days after injection because of the reutilization of labeled 16:0 acyl groups for lipid biosynthesis. Recycling of the acyl groups explains the long halflives reported for myelin phosphoglycerides after injection of [14C]acetic acid. Lipids with a relatively high specific radioactivity were lost from the myelin fraction during the purification procedure. The most likely source of these lipids is the most recently formed myelin that is not consolidated into the myelin sheath. |
topic |
phosphoglycerides turnover acyl groups microsomes myelin |
url |
http://www.sciencedirect.com/science/article/pii/S002222752036908X |
work_keys_str_mv |
AT graceysun metabolismofpalmiticacidinthesubcellularfractionsofmousebrain AT lloydahorrocks metabolismofpalmiticacidinthesubcellularfractionsofmousebrain |
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1721511828075315200 |