MicroRNA-103 Protects Coronary Artery Endothelial Cells against H2O2-Induced Oxidative Stress via BNIP3-Mediated End-Stage Autophagy and Antipyroptosis Pathways
Endothelial cell damage caused by oxidative stress is widely considered to be a triggering event in atherosclerosis (AS). However, the specific effect elicited by autophagy in endothelial cells undergoing oxidative stress remains controversial, especially during end-stage autophagy. The inhibition o...
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doaj-e3f522998e7246f0b3547b7aa7f3bcda2020-11-25T01:53:43ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942020-01-01202010.1155/2020/83513428351342MicroRNA-103 Protects Coronary Artery Endothelial Cells against H2O2-Induced Oxidative Stress via BNIP3-Mediated End-Stage Autophagy and Antipyroptosis PathwaysYiran Wang0Xianjing Song1Zhibo Li2Ning Liu3Youyou Yan4Tianyi Li5Wei Sun6Yinuo Guan7Ming Li8Yibo Yang9Xingru Yang10Bin Liu11Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaEndothelial cell damage caused by oxidative stress is widely considered to be a triggering event in atherosclerosis (AS). However, the specific effect elicited by autophagy in endothelial cells undergoing oxidative stress remains controversial, especially during end-stage autophagy. The inhibition of end-stage autophagy has been reported to increase cell pyroptosis and contribute to endothelial damage. Several studies have shown that microRNA-103 is involved in end-stage autophagy; however, its specific mechanism of action is not yet characterized. In this study, we addressed the regulatory role of miR-103 in autophagy during oxidative stress of endothelial cells. Hydrogen peroxide (H2O2) treatment was used as an in vitro model of oxidative stress. MTS and ROS levels were measured to evaluate cell activity. qRT-PCR was used to detect the expression of miR-103. Autophagy was examined using western blot, immunofluorescence staining, and electron microscopy, while western blot analysis detected pyroptosis-related proteins. Results show that miR-103 expression decreased under oxidative stress. Further, miR-103 repressed transcription of Bcl-2/adenovirus E1B 19 kDa interacting protein (BNIP3). The oxidative stress caused by H2O2 caused cell damage from 2 hours (P<0.05) and increased the level of intracellular reactive oxygen species (P<0.05); at the same time, the damage could be further aggravated by the stimulation of bafA1 (P<0.05). Under the stimulation of H2O2, the expression of miR-103 decreased (P<0.05). However, high expression of miR-103 could reduce the accumulation of LC3II and P62 (P<0.05) by inhibiting the downstream target gene Bcl-2/adenovirus E1B 19 kDa interacting protein (BNIP3), thus reducing the occurrence of cell pyroptosis (P<0.05). This process could be blocked by end-stage autophagy inhibitor bafA1 (P<0.05), which further indicated that miR-103 affected cell injury by autophagy. On the contrary, the low expression of miR-103 promoted the accumulation of autophagy protein and increased the occurrence of pyroptosis (P<0.05). In conclusion, inhibition of miR-103 restrained end-stage of autophagy by regulating BNIP3, thus changing the occurrence of cell pyroptosis.http://dx.doi.org/10.1155/2020/8351342 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yiran Wang Xianjing Song Zhibo Li Ning Liu Youyou Yan Tianyi Li Wei Sun Yinuo Guan Ming Li Yibo Yang Xingru Yang Bin Liu |
spellingShingle |
Yiran Wang Xianjing Song Zhibo Li Ning Liu Youyou Yan Tianyi Li Wei Sun Yinuo Guan Ming Li Yibo Yang Xingru Yang Bin Liu MicroRNA-103 Protects Coronary Artery Endothelial Cells against H2O2-Induced Oxidative Stress via BNIP3-Mediated End-Stage Autophagy and Antipyroptosis Pathways Oxidative Medicine and Cellular Longevity |
author_facet |
Yiran Wang Xianjing Song Zhibo Li Ning Liu Youyou Yan Tianyi Li Wei Sun Yinuo Guan Ming Li Yibo Yang Xingru Yang Bin Liu |
author_sort |
Yiran Wang |
title |
MicroRNA-103 Protects Coronary Artery Endothelial Cells against H2O2-Induced Oxidative Stress via BNIP3-Mediated End-Stage Autophagy and Antipyroptosis Pathways |
title_short |
MicroRNA-103 Protects Coronary Artery Endothelial Cells against H2O2-Induced Oxidative Stress via BNIP3-Mediated End-Stage Autophagy and Antipyroptosis Pathways |
title_full |
MicroRNA-103 Protects Coronary Artery Endothelial Cells against H2O2-Induced Oxidative Stress via BNIP3-Mediated End-Stage Autophagy and Antipyroptosis Pathways |
title_fullStr |
MicroRNA-103 Protects Coronary Artery Endothelial Cells against H2O2-Induced Oxidative Stress via BNIP3-Mediated End-Stage Autophagy and Antipyroptosis Pathways |
title_full_unstemmed |
MicroRNA-103 Protects Coronary Artery Endothelial Cells against H2O2-Induced Oxidative Stress via BNIP3-Mediated End-Stage Autophagy and Antipyroptosis Pathways |
title_sort |
microrna-103 protects coronary artery endothelial cells against h2o2-induced oxidative stress via bnip3-mediated end-stage autophagy and antipyroptosis pathways |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2020-01-01 |
description |
Endothelial cell damage caused by oxidative stress is widely considered to be a triggering event in atherosclerosis (AS). However, the specific effect elicited by autophagy in endothelial cells undergoing oxidative stress remains controversial, especially during end-stage autophagy. The inhibition of end-stage autophagy has been reported to increase cell pyroptosis and contribute to endothelial damage. Several studies have shown that microRNA-103 is involved in end-stage autophagy; however, its specific mechanism of action is not yet characterized. In this study, we addressed the regulatory role of miR-103 in autophagy during oxidative stress of endothelial cells. Hydrogen peroxide (H2O2) treatment was used as an in vitro model of oxidative stress. MTS and ROS levels were measured to evaluate cell activity. qRT-PCR was used to detect the expression of miR-103. Autophagy was examined using western blot, immunofluorescence staining, and electron microscopy, while western blot analysis detected pyroptosis-related proteins. Results show that miR-103 expression decreased under oxidative stress. Further, miR-103 repressed transcription of Bcl-2/adenovirus E1B 19 kDa interacting protein (BNIP3). The oxidative stress caused by H2O2 caused cell damage from 2 hours (P<0.05) and increased the level of intracellular reactive oxygen species (P<0.05); at the same time, the damage could be further aggravated by the stimulation of bafA1 (P<0.05). Under the stimulation of H2O2, the expression of miR-103 decreased (P<0.05). However, high expression of miR-103 could reduce the accumulation of LC3II and P62 (P<0.05) by inhibiting the downstream target gene Bcl-2/adenovirus E1B 19 kDa interacting protein (BNIP3), thus reducing the occurrence of cell pyroptosis (P<0.05). This process could be blocked by end-stage autophagy inhibitor bafA1 (P<0.05), which further indicated that miR-103 affected cell injury by autophagy. On the contrary, the low expression of miR-103 promoted the accumulation of autophagy protein and increased the occurrence of pyroptosis (P<0.05). In conclusion, inhibition of miR-103 restrained end-stage of autophagy by regulating BNIP3, thus changing the occurrence of cell pyroptosis. |
url |
http://dx.doi.org/10.1155/2020/8351342 |
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