Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells
Cartilage tissue engineering is a promising option for repairing cartilage defects, although harvesting a large number of seeding cells remains a major challenge. Cartilage stem/progenitor cells (CSPCs) seem to be a promising cell source. Hypoxic extracellular vesicles (EVs) may play a major role in...
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Taylor & Francis Group
2021-01-01
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Online Access: | http://dx.doi.org/10.1080/21623945.2021.1945210 |
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doaj-e404e61b404a4bec86d6875c9da918412021-07-06T12:16:10ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2021-01-0110132233710.1080/21623945.2021.19452101945210Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cellsKe Xue0Yongkang Jiang1Xiaodie Zhang2Jun Wu3Lin Qi4Kai Liu5Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue EngineeringShanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue EngineeringShanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue EngineeringThe First People’s Hospital of ChangzhouHuadong Hospital, Fudan UniversityShanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue EngineeringCartilage tissue engineering is a promising option for repairing cartilage defects, although harvesting a large number of seeding cells remains a major challenge. Cartilage stem/progenitor cells (CSPCs) seem to be a promising cell source. Hypoxic extracellular vesicles (EVs) may play a major role in cell-cell and tissue-tissue communication. In the current study, we aimed to evaluate the effect of hypoxic adipose-derived stem cells (ADSCs)-derived EVs on CSPCs proliferation and differentiation. The characteristics of ADSCs-derived EVs were identified, and proliferation, migration, and cartilage-related gene expression of CSPCs were measured with or without the presence of hypoxic ADSCs-derived EVs. SEM, histological staining, biochemical and biomechanical analysis was performed to evaluate the effect of hypoxic ADSCs-derived EVs on CSPCs in alginate hydrogel culture. The results indicated that the majority of ADSC-derived EVs exhibited a round-shaped or cup-shaped morphology with a diameter of 40–1000 nm and expressed CD9, CD63, and CD81. CSPCs migration and proliferation were enhanced by hypoxic ADSCs-derived EVs, which also increased the expression of cartilage-related genes. The hypoxic ADSCs-derived EVs induce CSPCs to produce significantly more cartilage matrix and proteoglycan. In conclusion, hypoxic ADSCs-derived EVs improved the proliferation and chondrogenic differentiation of CSPCs for cartilage tissue engineering.http://dx.doi.org/10.1080/21623945.2021.1945210cartilage stem/progenitor cellsextracellular vesicleshypoxic adipose-derived stem cellscartilage tissue engineeringproliferationdifferentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ke Xue Yongkang Jiang Xiaodie Zhang Jun Wu Lin Qi Kai Liu |
spellingShingle |
Ke Xue Yongkang Jiang Xiaodie Zhang Jun Wu Lin Qi Kai Liu Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells Adipocyte cartilage stem/progenitor cells extracellular vesicles hypoxic adipose-derived stem cells cartilage tissue engineering proliferation differentiation |
author_facet |
Ke Xue Yongkang Jiang Xiaodie Zhang Jun Wu Lin Qi Kai Liu |
author_sort |
Ke Xue |
title |
Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells |
title_short |
Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells |
title_full |
Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells |
title_fullStr |
Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells |
title_full_unstemmed |
Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells |
title_sort |
hypoxic adscs-derived evs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells |
publisher |
Taylor & Francis Group |
series |
Adipocyte |
issn |
2162-3945 2162-397X |
publishDate |
2021-01-01 |
description |
Cartilage tissue engineering is a promising option for repairing cartilage defects, although harvesting a large number of seeding cells remains a major challenge. Cartilage stem/progenitor cells (CSPCs) seem to be a promising cell source. Hypoxic extracellular vesicles (EVs) may play a major role in cell-cell and tissue-tissue communication. In the current study, we aimed to evaluate the effect of hypoxic adipose-derived stem cells (ADSCs)-derived EVs on CSPCs proliferation and differentiation. The characteristics of ADSCs-derived EVs were identified, and proliferation, migration, and cartilage-related gene expression of CSPCs were measured with or without the presence of hypoxic ADSCs-derived EVs. SEM, histological staining, biochemical and biomechanical analysis was performed to evaluate the effect of hypoxic ADSCs-derived EVs on CSPCs in alginate hydrogel culture. The results indicated that the majority of ADSC-derived EVs exhibited a round-shaped or cup-shaped morphology with a diameter of 40–1000 nm and expressed CD9, CD63, and CD81. CSPCs migration and proliferation were enhanced by hypoxic ADSCs-derived EVs, which also increased the expression of cartilage-related genes. The hypoxic ADSCs-derived EVs induce CSPCs to produce significantly more cartilage matrix and proteoglycan. In conclusion, hypoxic ADSCs-derived EVs improved the proliferation and chondrogenic differentiation of CSPCs for cartilage tissue engineering. |
topic |
cartilage stem/progenitor cells extracellular vesicles hypoxic adipose-derived stem cells cartilage tissue engineering proliferation differentiation |
url |
http://dx.doi.org/10.1080/21623945.2021.1945210 |
work_keys_str_mv |
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1721317479177781248 |