Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model
Collagenated porcine-derived bone graft materials exhibit osteoconductive properties and the development of different formulations intends to enhance bone regeneration. This study aims to evaluate bone healing in a rabbit cancellous bone defect in response to grafting with different physicochemical...
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doaj-e419a4bed80f4d30b9dfc61219cf2b712021-03-03T00:05:45ZengMDPI AGMolecules1420-30492021-03-01261339133910.3390/molecules26051339Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone ModelRui I. Falacho0Paulo J. Palma1Joana A. Marques2Maria H. Figueiredo3Francisco Caramelo4Isabel Dias5Carlos Viegas6Fernando Guerra7Institute of Oral Implantology and Prosthodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, PortugalInstitute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, PortugalInstitute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, PortugalDentistry Department, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, PortugalCoimbra Institute for Clinical and Biomedical Research (iCBR), Laboratory of Biostatistics and Medical Informatics (LBIM), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalDepartment of Veterinary Sciences, School of Agricultural and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalDepartment of Veterinary Sciences, School of Agricultural and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalInstitute of Oral Implantology and Prosthodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, PortugalCollagenated porcine-derived bone graft materials exhibit osteoconductive properties and the development of different formulations intends to enhance bone regeneration. This study aims to evaluate bone healing in a rabbit cancellous bone defect in response to grafting with different physicochemical forms of heterologous porcine bone. Twenty-six adult male New Zealand White rabbits received two critical size femoral bone defects per animal (n = 52), each randomly assigned to one of the five tested materials (Apatos, Gen-Os, mp3, Putty, and Gel 40). Animals were sacrificed at 15- and 30-days post-surgery. Qualitative and quantitative (new bone, particle and connective tissue percentages) histological analyses were performed. Histomorphometry showed statistically significant differences in all evaluated parameters between mp3 and both Putty and Gel 40 groups, regardless of the timepoint (<i>p </i>< 0.05). Moreover, statistical differences were observed between Apatos and both Putty (<i>p </i>= 0.014) and Gel 40 (<i>p </i>= 0.007) groups, at 30 days, in regard to particle percentage. Within each group, regarding new bone formation, mp3 showed significant differences (<i>p </i>= 0.028) between 15 (40.93 ± 3.49%) and 30 (52.49 ± 11.04%) days. Additionally, intragroup analysis concerning the percentage of particles revealed a significant reduction in particle occupied area from 15 to 30 days in mp3 and Gen-Os groups (<i>p</i> = 0.009). All mp3, Gen-Os and Apatos exhibited promising results in terms of new bone formation, thus presenting suitable alternatives to be used in bone regeneration.https://www.mdpi.com/1420-3049/26/5/1339bone graftingbone regenerationcollagenated bonehistomorphometryporcine bone graftrabbit model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rui I. Falacho Paulo J. Palma Joana A. Marques Maria H. Figueiredo Francisco Caramelo Isabel Dias Carlos Viegas Fernando Guerra |
spellingShingle |
Rui I. Falacho Paulo J. Palma Joana A. Marques Maria H. Figueiredo Francisco Caramelo Isabel Dias Carlos Viegas Fernando Guerra Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model Molecules bone grafting bone regeneration collagenated bone histomorphometry porcine bone graft rabbit model |
author_facet |
Rui I. Falacho Paulo J. Palma Joana A. Marques Maria H. Figueiredo Francisco Caramelo Isabel Dias Carlos Viegas Fernando Guerra |
author_sort |
Rui I. Falacho |
title |
Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model |
title_short |
Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model |
title_full |
Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model |
title_fullStr |
Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model |
title_full_unstemmed |
Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model |
title_sort |
collagenated porcine heterologous bone grafts: histomorphometric evaluation of bone formation using different physical forms in a rabbit cancellous bone model |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-03-01 |
description |
Collagenated porcine-derived bone graft materials exhibit osteoconductive properties and the development of different formulations intends to enhance bone regeneration. This study aims to evaluate bone healing in a rabbit cancellous bone defect in response to grafting with different physicochemical forms of heterologous porcine bone. Twenty-six adult male New Zealand White rabbits received two critical size femoral bone defects per animal (n = 52), each randomly assigned to one of the five tested materials (Apatos, Gen-Os, mp3, Putty, and Gel 40). Animals were sacrificed at 15- and 30-days post-surgery. Qualitative and quantitative (new bone, particle and connective tissue percentages) histological analyses were performed. Histomorphometry showed statistically significant differences in all evaluated parameters between mp3 and both Putty and Gel 40 groups, regardless of the timepoint (<i>p </i>< 0.05). Moreover, statistical differences were observed between Apatos and both Putty (<i>p </i>= 0.014) and Gel 40 (<i>p </i>= 0.007) groups, at 30 days, in regard to particle percentage. Within each group, regarding new bone formation, mp3 showed significant differences (<i>p </i>= 0.028) between 15 (40.93 ± 3.49%) and 30 (52.49 ± 11.04%) days. Additionally, intragroup analysis concerning the percentage of particles revealed a significant reduction in particle occupied area from 15 to 30 days in mp3 and Gen-Os groups (<i>p</i> = 0.009). All mp3, Gen-Os and Apatos exhibited promising results in terms of new bone formation, thus presenting suitable alternatives to be used in bone regeneration. |
topic |
bone grafting bone regeneration collagenated bone histomorphometry porcine bone graft rabbit model |
url |
https://www.mdpi.com/1420-3049/26/5/1339 |
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