Changing trends in epidemiology and antifungal susceptibility patterns of six bloodstream Candida species isolates over a 12-year period in Kuwait.
Changing trends in incidence and antifungal susceptibility patterns of six Candida species causing candidemia in Kuwait between 2006-2017 are reported. A total of 2075 isolates obtained from 1448 patients were analyzed. Identity of Candida species isolates was determined by phenotypic methods and co...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0216250 |
id |
doaj-e41fb70337474fd6a698dbfa6a1d512b |
---|---|
record_format |
Article |
spelling |
doaj-e41fb70337474fd6a698dbfa6a1d512b2021-03-03T20:42:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01145e021625010.1371/journal.pone.0216250Changing trends in epidemiology and antifungal susceptibility patterns of six bloodstream Candida species isolates over a 12-year period in Kuwait.Ziauddin KhanSuhail AhmadNoura Al-SweihEiman MokaddasKhalifa Al-BanwanWadha AlfouzanInaam Al-ObaidKhaled Al-ObaidMohammad AsadzadehAhlam JeraghLeena JosephSoumya VargheseSandhya VayalilOmar Al-MusallamChanging trends in incidence and antifungal susceptibility patterns of six Candida species causing candidemia in Kuwait between 2006-2017 are reported. A total of 2075 isolates obtained from 1448 patients were analyzed. Identity of Candida species isolates was determined by phenotypic methods and confirmed by PCR amplification/PCR-sequencing of rDNA and/or MALDI-TOF MS. Antifungal susceptibility was determined by Etest. C. albicans accounted for 539 (37.22%) cases followed by C. parapsilosis (n = 502, 34.67%), C. tropicalis (n = 210, 14.5%), C. glabrata (n = 148, 10.22%), C. krusei (n = 27, 1.81%) and C. dubliniensis (n = 22, 1.5%). The comparative percent distribution of Candida species causing candidemia between 2006-2011 and 2012-2017 was as follows: C. albicans 41.8% and 33.1%, C. parapsilosis complex 32.01% and 37.04%, C. tropicalis 13.59% and 15.31%, and C. glabrata 8.77% and 11.51%, C. krusei 2.0% and 1.7%, and C. dubliniensis 1.75 and 1.3%, respectively. Three of 371 C. albicans isolates during 2006-2011 and five of 363 during 2012-2017 were resistant to fluconazole. Among C. parapsilosis isolates, one of 310 during 2006-2011 and 21 of 446 during 2012-2017 were resistant to this drug. Furthermore, at an epidemiologic cutoff value (ECV) of ≤0.5 μg/ml, 70.1% C. albicans isolates were wild-type for fluconazole during 2006-2011 as compared to 58.1% during 2012-2017. Likewise, at an ECV of ≤2 μg/ml, 98.0% of C. parapsilosis isolates were wild-type during 2006-2011 as compared to 93.4% during 2012-2017. Clonal spread of fluconazole-resistant C. parapsilosis in one major hospital was documented. An 8.8% shift in favor of non-albicans Candida species with concomitant increase in MICs between the two periods preludes emergence of fluconazole-resistant candidemia cases in Kuwait.https://doi.org/10.1371/journal.pone.0216250 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ziauddin Khan Suhail Ahmad Noura Al-Sweih Eiman Mokaddas Khalifa Al-Banwan Wadha Alfouzan Inaam Al-Obaid Khaled Al-Obaid Mohammad Asadzadeh Ahlam Jeragh Leena Joseph Soumya Varghese Sandhya Vayalil Omar Al-Musallam |
spellingShingle |
Ziauddin Khan Suhail Ahmad Noura Al-Sweih Eiman Mokaddas Khalifa Al-Banwan Wadha Alfouzan Inaam Al-Obaid Khaled Al-Obaid Mohammad Asadzadeh Ahlam Jeragh Leena Joseph Soumya Varghese Sandhya Vayalil Omar Al-Musallam Changing trends in epidemiology and antifungal susceptibility patterns of six bloodstream Candida species isolates over a 12-year period in Kuwait. PLoS ONE |
author_facet |
Ziauddin Khan Suhail Ahmad Noura Al-Sweih Eiman Mokaddas Khalifa Al-Banwan Wadha Alfouzan Inaam Al-Obaid Khaled Al-Obaid Mohammad Asadzadeh Ahlam Jeragh Leena Joseph Soumya Varghese Sandhya Vayalil Omar Al-Musallam |
author_sort |
Ziauddin Khan |
title |
Changing trends in epidemiology and antifungal susceptibility patterns of six bloodstream Candida species isolates over a 12-year period in Kuwait. |
title_short |
Changing trends in epidemiology and antifungal susceptibility patterns of six bloodstream Candida species isolates over a 12-year period in Kuwait. |
title_full |
Changing trends in epidemiology and antifungal susceptibility patterns of six bloodstream Candida species isolates over a 12-year period in Kuwait. |
title_fullStr |
Changing trends in epidemiology and antifungal susceptibility patterns of six bloodstream Candida species isolates over a 12-year period in Kuwait. |
title_full_unstemmed |
Changing trends in epidemiology and antifungal susceptibility patterns of six bloodstream Candida species isolates over a 12-year period in Kuwait. |
title_sort |
changing trends in epidemiology and antifungal susceptibility patterns of six bloodstream candida species isolates over a 12-year period in kuwait. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
Changing trends in incidence and antifungal susceptibility patterns of six Candida species causing candidemia in Kuwait between 2006-2017 are reported. A total of 2075 isolates obtained from 1448 patients were analyzed. Identity of Candida species isolates was determined by phenotypic methods and confirmed by PCR amplification/PCR-sequencing of rDNA and/or MALDI-TOF MS. Antifungal susceptibility was determined by Etest. C. albicans accounted for 539 (37.22%) cases followed by C. parapsilosis (n = 502, 34.67%), C. tropicalis (n = 210, 14.5%), C. glabrata (n = 148, 10.22%), C. krusei (n = 27, 1.81%) and C. dubliniensis (n = 22, 1.5%). The comparative percent distribution of Candida species causing candidemia between 2006-2011 and 2012-2017 was as follows: C. albicans 41.8% and 33.1%, C. parapsilosis complex 32.01% and 37.04%, C. tropicalis 13.59% and 15.31%, and C. glabrata 8.77% and 11.51%, C. krusei 2.0% and 1.7%, and C. dubliniensis 1.75 and 1.3%, respectively. Three of 371 C. albicans isolates during 2006-2011 and five of 363 during 2012-2017 were resistant to fluconazole. Among C. parapsilosis isolates, one of 310 during 2006-2011 and 21 of 446 during 2012-2017 were resistant to this drug. Furthermore, at an epidemiologic cutoff value (ECV) of ≤0.5 μg/ml, 70.1% C. albicans isolates were wild-type for fluconazole during 2006-2011 as compared to 58.1% during 2012-2017. Likewise, at an ECV of ≤2 μg/ml, 98.0% of C. parapsilosis isolates were wild-type during 2006-2011 as compared to 93.4% during 2012-2017. Clonal spread of fluconazole-resistant C. parapsilosis in one major hospital was documented. An 8.8% shift in favor of non-albicans Candida species with concomitant increase in MICs between the two periods preludes emergence of fluconazole-resistant candidemia cases in Kuwait. |
url |
https://doi.org/10.1371/journal.pone.0216250 |
work_keys_str_mv |
AT ziauddinkhan changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT suhailahmad changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT nouraalsweih changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT eimanmokaddas changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT khalifaalbanwan changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT wadhaalfouzan changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT inaamalobaid changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT khaledalobaid changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT mohammadasadzadeh changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT ahlamjeragh changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT leenajoseph changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT soumyavarghese changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT sandhyavayalil changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait AT omaralmusallam changingtrendsinepidemiologyandantifungalsusceptibilitypatternsofsixbloodstreamcandidaspeciesisolatesovera12yearperiodinkuwait |
_version_ |
1714821090069446656 |