Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin’s Disease Prior to Cancer Therapy
Purpose: Hodgkin’s disease (HD) is a type of cancer affecting men in the reproductive age with potential consequences on their fertility status. This study aims to analyze sperm parameters, alterations in proteomic profiles and validate selected protein biomarkers of spermatozoa in men with HD und...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Korean Society for Sexual Medicine and Andrology
2020-10-01
|
Series: | The World Journal of Men's Health |
Subjects: |
id |
doaj-e42a51e771a349bbbcd2ff2a1541e410 |
---|---|
record_format |
Article |
spelling |
doaj-e42a51e771a349bbbcd2ff2a1541e4102020-11-25T03:06:49ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902020-10-0138452153410.5534/wjmh.190012Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin’s Disease Prior to Cancer TherapyAna D. Martins0https://orcid.org/0000-0002-7261-9596Ashok Agarwal1https://orcid.org/0000-0003-0585-1026Saradha Baskaran2https://orcid.org/0000-0002-4499-8680Peter Natesan Pushparaj3https://orcid.org/0000-0001-7574-1880Gulfam Ahmad4https://orcid.org/0000-0002-4225-8347Manesh Kumar Panner Selvam5https://orcid.org/0000-0002-9120-2278Cleveland ClinicCleveland ClinicCleveland ClinicKing Abdulaziz UniversitySydney UniversityCleveland ClinicPurpose: Hodgkin’s disease (HD) is a type of cancer affecting men in the reproductive age with potential consequences on their fertility status. This study aims to analyze sperm parameters, alterations in proteomic profiles and validate selected protein biomarkers of spermatozoa in men with HD undergoing sperm banking before cancer therapy. Materials and Methods: Semen analysis was carried out in healthy fertile donors (control, n=42), and patients diagnosed with HD (patients, n=38) before cancer therapy. We compared proteomic profiles of spermatozoa from donors (n=3) and patients (n=3) using LTQ-Orbitrap Elite hybrid MS system. Results: A total of 1,169 proteins were identified by global proteomic in both groups. The ingenuity pathway analysis revealed that differentially expressed proteins involved in capacitation, acrosome reaction, binding of sperm to the zona pellucida, sperm motility, regulation of sperm DNA damage, and apoptosis were significantly downregulated in HD patients. Validation of proteins implicated in sperm fertility potential by Western Blot demonstrated that peroxiredoxin 2 (PRDX 2) was underexpressed (p=0.015), and transferrin (p=0.045) and SERPIN A5 (p=0.010) protein levels were overexpressed in spermatozoa of men with HD. Conclusions: Findings of this study indicates that the key proteins involved in sperm fertility potential are significantly altered in men with HD, which provides substantial explanation for the observed low sperm quality in HD subjects prior to cancer therapy. Furthermore, our results suggest PRDX 2, transferrin and SERPIN A5 as possible candidate proteins for assessing sperm quality in HD patients prior to cancer therapy.hodgkin diseaseinfertilitymass spectrometryproteomicsspermatozoatransferrin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana D. Martins Ashok Agarwal Saradha Baskaran Peter Natesan Pushparaj Gulfam Ahmad Manesh Kumar Panner Selvam |
spellingShingle |
Ana D. Martins Ashok Agarwal Saradha Baskaran Peter Natesan Pushparaj Gulfam Ahmad Manesh Kumar Panner Selvam Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin’s Disease Prior to Cancer Therapy The World Journal of Men's Health hodgkin disease infertility mass spectrometry proteomics spermatozoa transferrin |
author_facet |
Ana D. Martins Ashok Agarwal Saradha Baskaran Peter Natesan Pushparaj Gulfam Ahmad Manesh Kumar Panner Selvam |
author_sort |
Ana D. Martins |
title |
Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin’s Disease Prior to Cancer Therapy |
title_short |
Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin’s Disease Prior to Cancer Therapy |
title_full |
Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin’s Disease Prior to Cancer Therapy |
title_fullStr |
Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin’s Disease Prior to Cancer Therapy |
title_full_unstemmed |
Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin’s Disease Prior to Cancer Therapy |
title_sort |
alterations of spermatozoa proteomic profile in men with hodgkin’s disease prior to cancer therapy |
publisher |
Korean Society for Sexual Medicine and Andrology |
series |
The World Journal of Men's Health |
issn |
2287-4208 2287-4690 |
publishDate |
2020-10-01 |
description |
Purpose: Hodgkin’s disease (HD) is a type of cancer affecting men in the reproductive age with potential consequences on
their fertility status. This study aims to analyze sperm parameters, alterations in proteomic profiles and validate selected protein
biomarkers of spermatozoa in men with HD undergoing sperm banking before cancer therapy.
Materials and Methods: Semen analysis was carried out in healthy fertile donors (control, n=42), and patients diagnosed with
HD (patients, n=38) before cancer therapy. We compared proteomic profiles of spermatozoa from donors (n=3) and patients
(n=3) using LTQ-Orbitrap Elite hybrid MS system.
Results: A total of 1,169 proteins were identified by global proteomic in both groups. The ingenuity pathway analysis revealed
that differentially expressed proteins involved in capacitation, acrosome reaction, binding of sperm to the zona pellucida,
sperm motility, regulation of sperm DNA damage, and apoptosis were significantly downregulated in HD patients.
Validation of proteins implicated in sperm fertility potential by Western Blot demonstrated that peroxiredoxin 2 (PRDX 2) was
underexpressed (p=0.015), and transferrin (p=0.045) and SERPIN A5 (p=0.010) protein levels were overexpressed in spermatozoa
of men with HD.
Conclusions: Findings of this study indicates that the key proteins involved in sperm fertility potential are significantly altered
in men with HD, which provides substantial explanation for the observed low sperm quality in HD subjects prior to cancer
therapy. Furthermore, our results suggest PRDX 2, transferrin and SERPIN A5 as possible candidate proteins for assessing
sperm quality in HD patients prior to cancer therapy. |
topic |
hodgkin disease infertility mass spectrometry proteomics spermatozoa transferrin |
work_keys_str_mv |
AT anadmartins alterationsofspermatozoaproteomicprofileinmenwithhodgkinsdiseasepriortocancertherapy AT ashokagarwal alterationsofspermatozoaproteomicprofileinmenwithhodgkinsdiseasepriortocancertherapy AT saradhabaskaran alterationsofspermatozoaproteomicprofileinmenwithhodgkinsdiseasepriortocancertherapy AT peternatesanpushparaj alterationsofspermatozoaproteomicprofileinmenwithhodgkinsdiseasepriortocancertherapy AT gulfamahmad alterationsofspermatozoaproteomicprofileinmenwithhodgkinsdiseasepriortocancertherapy AT maneshkumarpannerselvam alterationsofspermatozoaproteomicprofileinmenwithhodgkinsdiseasepriortocancertherapy |
_version_ |
1724672129595604992 |