Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways
Objective. Parathyroid hormone (PTH) is considered to be essential during the tooth development. Stem cells from the apical papilla (SCAPs) are responsible for dentine formation. However, the interaction between PTH and SCAPs remains unclear. This study was aimed at investigating the effects of PTH...
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doaj-e4357ba9fbfc4de385ab6d8ace05d03d2020-11-25T02:57:28ZengHindawi LimitedStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/51281285128128Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK PathwaysXiyao Pang0Ying Zhuang1Zehan Li2Shuanglin Jing3Qin Cai4Fengge Zhang5Changao Xue6Jinhua Yu7Department of Stomatology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, ChinaDepartment of Stomatology, Binhai County People’s Hospital, Yancheng, Jiangsu, ChinaEndodontic Department, School of Stomatology, Nanjing Medical University, Nanjing, Jiangsu, ChinaEndodontic Department, School of Stomatology, Nanjing Medical University, Nanjing, Jiangsu, ChinaDepartment of Stomatology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, ChinaDepartment of Stomatology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, ChinaDepartment of Stomatology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, ChinaEndodontic Department, School of Stomatology, Nanjing Medical University, Nanjing, Jiangsu, ChinaObjective. Parathyroid hormone (PTH) is considered to be essential during the tooth development. Stem cells from the apical papilla (SCAPs) are responsible for dentine formation. However, the interaction between PTH and SCAPs remains unclear. This study was aimed at investigating the effects of PTH on odonto/osteogenic differentiation capacity of SCAPs and elucidating the underlying molecular mechanisms. Materials and Methods. Here, SCAPs were isolated and identified in vitro. Effects of PTH on the proliferation of SCAPs were determined by Cell Counting Kit-8 (CCK-8), flow cytometry (FCM), and EdU. Alkaline phosphatase (ALP) activity, alizarin red staining, Western blot, and RT-PCR were carried out to detect the odonto/osteogenic differentiation of PTH-treated SCAPs as well as the participation of the MAPK signaling pathway. Results. An ALP activity assay determined that 10-8 mol/L PTH was the optimal concentration for the induction of SCAPs with no significant influence on the proliferation of SCAPs as indicated by CCK-8, FCM, and EdU. The expression of odonto/osteogenic markers was significantly upregulated in mRNA levels and protein levels. Moreover, intermittent treatment of PTH also increased phosphorylation of JNK and P38, and the differentiation was suppressed following the inhibition of JNK and P38 MAPK pathways. Conclusion. PTH can regulate the odonto/osteogenic differentiation of SCAPs via JNK and P38 MAPK pathways.http://dx.doi.org/10.1155/2020/5128128 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiyao Pang Ying Zhuang Zehan Li Shuanglin Jing Qin Cai Fengge Zhang Changao Xue Jinhua Yu |
spellingShingle |
Xiyao Pang Ying Zhuang Zehan Li Shuanglin Jing Qin Cai Fengge Zhang Changao Xue Jinhua Yu Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways Stem Cells International |
author_facet |
Xiyao Pang Ying Zhuang Zehan Li Shuanglin Jing Qin Cai Fengge Zhang Changao Xue Jinhua Yu |
author_sort |
Xiyao Pang |
title |
Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways |
title_short |
Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways |
title_full |
Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways |
title_fullStr |
Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways |
title_full_unstemmed |
Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways |
title_sort |
intermittent administration of parathyroid hormone enhances odonto/osteogenic differentiation of stem cells from the apical papilla via jnk and p38 mapk pathways |
publisher |
Hindawi Limited |
series |
Stem Cells International |
issn |
1687-966X 1687-9678 |
publishDate |
2020-01-01 |
description |
Objective. Parathyroid hormone (PTH) is considered to be essential during the tooth development. Stem cells from the apical papilla (SCAPs) are responsible for dentine formation. However, the interaction between PTH and SCAPs remains unclear. This study was aimed at investigating the effects of PTH on odonto/osteogenic differentiation capacity of SCAPs and elucidating the underlying molecular mechanisms. Materials and Methods. Here, SCAPs were isolated and identified in vitro. Effects of PTH on the proliferation of SCAPs were determined by Cell Counting Kit-8 (CCK-8), flow cytometry (FCM), and EdU. Alkaline phosphatase (ALP) activity, alizarin red staining, Western blot, and RT-PCR were carried out to detect the odonto/osteogenic differentiation of PTH-treated SCAPs as well as the participation of the MAPK signaling pathway. Results. An ALP activity assay determined that 10-8 mol/L PTH was the optimal concentration for the induction of SCAPs with no significant influence on the proliferation of SCAPs as indicated by CCK-8, FCM, and EdU. The expression of odonto/osteogenic markers was significantly upregulated in mRNA levels and protein levels. Moreover, intermittent treatment of PTH also increased phosphorylation of JNK and P38, and the differentiation was suppressed following the inhibition of JNK and P38 MAPK pathways. Conclusion. PTH can regulate the odonto/osteogenic differentiation of SCAPs via JNK and P38 MAPK pathways. |
url |
http://dx.doi.org/10.1155/2020/5128128 |
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