Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape

Abstract Background Aroma, berry firmness and berry shape are three main quality traits in table grape production, and also the important target traits in grapevine breeding. However, the information about their genetic mechanisms is limited, which results in low accuracy and efficiency of quality b...

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Main Authors: Huiling Wang, Ailing Yan, Lei Sun, Guojun Zhang, Xiaoyue Wang, Jiancheng Ren, Haiying Xu
Format: Article
Language:English
Published: BMC 2020-09-01
Series:BMC Plant Biology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12870-020-02630-x
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spelling doaj-e439f846addc4b468fe63616101018682020-11-25T03:24:55ZengBMCBMC Plant Biology1471-22292020-09-0120111510.1186/s12870-020-02630-xNovel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grapeHuiling Wang0Ailing Yan1Lei Sun2Guojun Zhang3Xiaoyue Wang4Jiancheng Ren5Haiying Xu6Beijing Academy of Forestry and Pomology SciencesBeijing Academy of Forestry and Pomology SciencesBeijing Academy of Forestry and Pomology SciencesBeijing Academy of Forestry and Pomology SciencesBeijing Academy of Forestry and Pomology SciencesBeijing Academy of Forestry and Pomology SciencesBeijing Academy of Forestry and Pomology SciencesAbstract Background Aroma, berry firmness and berry shape are three main quality traits in table grape production, and also the important target traits in grapevine breeding. However, the information about their genetic mechanisms is limited, which results in low accuracy and efficiency of quality breeding in grapevine. Mapping and isolation of quantitative trait locus (QTLs) based on the construction of genetic linkage map is a powerful approach to decipher the genetic determinants of complex quantitative traits. Results In the present work, a final integrated map consisting of 3411 SLAF markers on 19 linkage groups (LGs) with an average distance of 0.98 cM between adjacent markers was generated using the specific length amplified fragment sequencing (SLAF-seq) technique. A total of 9 significant stable QTLs for Muscat flavor, berry firmness and berry shape were identified on two linkage groups among the hybrids analyzed over three consecutive years from 2016 to 2018. Notably, new stable QTLs for berry firmness and berry shape were found on LG 8 respectively for the first time. Based on biological function and expression profiles of candidate genes in the major QTL regions, 3 genes (VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350) related to berry firmness and 3 genes (VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200) linked to berry shape were highlighted. Overexpression of VIT_08s0032g01110 in transgenic Arabidopsis plants caused the change of pod shape. Conclusions A new high-density genetic map with total 3411 markers was constructed with SLAF-seq technique, and thus enabled the detection of narrow interval QTLs for relevant traits in grapevine. VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350 were found to be related to berry firmness, while VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200 were linked to berry shape.http://link.springer.com/article/10.1186/s12870-020-02630-xGrapevineHigh-density genetic linkage mapMuscat flavorBerry firmnessBerry shapeCandidate genes
collection DOAJ
language English
format Article
sources DOAJ
author Huiling Wang
Ailing Yan
Lei Sun
Guojun Zhang
Xiaoyue Wang
Jiancheng Ren
Haiying Xu
spellingShingle Huiling Wang
Ailing Yan
Lei Sun
Guojun Zhang
Xiaoyue Wang
Jiancheng Ren
Haiying Xu
Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
BMC Plant Biology
Grapevine
High-density genetic linkage map
Muscat flavor
Berry firmness
Berry shape
Candidate genes
author_facet Huiling Wang
Ailing Yan
Lei Sun
Guojun Zhang
Xiaoyue Wang
Jiancheng Ren
Haiying Xu
author_sort Huiling Wang
title Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_short Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_full Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_fullStr Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_full_unstemmed Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_sort novel stable qtls identification for berry quality traits based on high-density genetic linkage map construction in table grape
publisher BMC
series BMC Plant Biology
issn 1471-2229
publishDate 2020-09-01
description Abstract Background Aroma, berry firmness and berry shape are three main quality traits in table grape production, and also the important target traits in grapevine breeding. However, the information about their genetic mechanisms is limited, which results in low accuracy and efficiency of quality breeding in grapevine. Mapping and isolation of quantitative trait locus (QTLs) based on the construction of genetic linkage map is a powerful approach to decipher the genetic determinants of complex quantitative traits. Results In the present work, a final integrated map consisting of 3411 SLAF markers on 19 linkage groups (LGs) with an average distance of 0.98 cM between adjacent markers was generated using the specific length amplified fragment sequencing (SLAF-seq) technique. A total of 9 significant stable QTLs for Muscat flavor, berry firmness and berry shape were identified on two linkage groups among the hybrids analyzed over three consecutive years from 2016 to 2018. Notably, new stable QTLs for berry firmness and berry shape were found on LG 8 respectively for the first time. Based on biological function and expression profiles of candidate genes in the major QTL regions, 3 genes (VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350) related to berry firmness and 3 genes (VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200) linked to berry shape were highlighted. Overexpression of VIT_08s0032g01110 in transgenic Arabidopsis plants caused the change of pod shape. Conclusions A new high-density genetic map with total 3411 markers was constructed with SLAF-seq technique, and thus enabled the detection of narrow interval QTLs for relevant traits in grapevine. VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350 were found to be related to berry firmness, while VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200 were linked to berry shape.
topic Grapevine
High-density genetic linkage map
Muscat flavor
Berry firmness
Berry shape
Candidate genes
url http://link.springer.com/article/10.1186/s12870-020-02630-x
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