Improvement of Salt Tolerance Using Wild Rice Genes
Salt stress causes significant reductions in rice production worldwide; thus, improving salt tolerance is a promising approach to meet the increasing food demand. Wild rice germplasm is considered a valuable genetic resource for improving rice cultivars. However, information regarding the improvemen...
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doaj-e44a5d4b4f4240c891639bff0091bdd02020-11-24T23:01:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-01-01810.3389/fpls.2017.02269322548Improvement of Salt Tolerance Using Wild Rice GenesRuidang Quan0Ruidang Quan1Juan Wang2Juan Wang3Jian Hui4Haibo Bai5Xuelian Lyu6Yongxing Zhu7Haiwen Zhang8Haiwen Zhang9Zhijin Zhang10Zhijin Zhang11Shuhua Li12Rongfeng Huang13Rongfeng Huang14Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, ChinaNingxia Academy of Agriculture and Forestry Sciences, Yinchuan, ChinaNingxia Academy of Agriculture and Forestry Sciences, Yinchuan, ChinaNingxia Academy of Agriculture and Forestry Sciences, Yinchuan, ChinaNingxia Academy of Agriculture and Forestry Sciences, Yinchuan, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, ChinaNingxia Academy of Agriculture and Forestry Sciences, Yinchuan, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, ChinaSalt stress causes significant reductions in rice production worldwide; thus, improving salt tolerance is a promising approach to meet the increasing food demand. Wild rice germplasm is considered a valuable genetic resource for improving rice cultivars. However, information regarding the improvement of salt tolerance in cultivated rice using wild rice genes is limited. In this study, we identified a salt-tolerant line Dongxiang/Ningjing 15 (DJ15) under salt-stress field conditions from the population of a salt tolerant Dongxiang wild rice × a cultivated rice variety Ningjing16 (NJ16). Genomic resequencing analysis of NJ16, DJ15 and Dongxiang wild rice revealed that the introgressed genomic fragments were unevenly distributed over the 12 chromosomes (Chr.) and mainly identified on Chr. 6, 7, 10, and 11. Using quantitative trait locus (QTL) mapping, we found 9 QTL for salt tolerance (qST) at the seedling stage located on Chr. 1, 3, 4, 5, 6, 8, and 10. In addition, sequence variant analysis within the QTL regions demonstrated that SKC1/HKT8/HKT1;5 and HAK6 transporters along with numerous transcriptional factors were the candidate genes for the salt tolerant QTL. The DJ15/Koshihikari recombinant inbred lines that contained both qST1.2 and qST6, two QTL with the highest effect for salt tolerance, were more tolerant than the parental lines under salt-stress field conditions. Furthermore, the qST6 near-isogenic lines with IR29 background were more tolerant than IR29, indicating that qST1.2 and qST6 could improve salt tolerance in rice. Overall, our study indicates that wild rice genes could markedly improve the salt tolerance of cultivated rice.http://journal.frontiersin.org/article/10.3389/fpls.2017.02269/fullwild ricecultivated ricesalt toleranceQTLgene introgression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ruidang Quan Ruidang Quan Juan Wang Juan Wang Jian Hui Haibo Bai Xuelian Lyu Yongxing Zhu Haiwen Zhang Haiwen Zhang Zhijin Zhang Zhijin Zhang Shuhua Li Rongfeng Huang Rongfeng Huang |
spellingShingle |
Ruidang Quan Ruidang Quan Juan Wang Juan Wang Jian Hui Haibo Bai Xuelian Lyu Yongxing Zhu Haiwen Zhang Haiwen Zhang Zhijin Zhang Zhijin Zhang Shuhua Li Rongfeng Huang Rongfeng Huang Improvement of Salt Tolerance Using Wild Rice Genes Frontiers in Plant Science wild rice cultivated rice salt tolerance QTL gene introgression |
author_facet |
Ruidang Quan Ruidang Quan Juan Wang Juan Wang Jian Hui Haibo Bai Xuelian Lyu Yongxing Zhu Haiwen Zhang Haiwen Zhang Zhijin Zhang Zhijin Zhang Shuhua Li Rongfeng Huang Rongfeng Huang |
author_sort |
Ruidang Quan |
title |
Improvement of Salt Tolerance Using Wild Rice Genes |
title_short |
Improvement of Salt Tolerance Using Wild Rice Genes |
title_full |
Improvement of Salt Tolerance Using Wild Rice Genes |
title_fullStr |
Improvement of Salt Tolerance Using Wild Rice Genes |
title_full_unstemmed |
Improvement of Salt Tolerance Using Wild Rice Genes |
title_sort |
improvement of salt tolerance using wild rice genes |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2018-01-01 |
description |
Salt stress causes significant reductions in rice production worldwide; thus, improving salt tolerance is a promising approach to meet the increasing food demand. Wild rice germplasm is considered a valuable genetic resource for improving rice cultivars. However, information regarding the improvement of salt tolerance in cultivated rice using wild rice genes is limited. In this study, we identified a salt-tolerant line Dongxiang/Ningjing 15 (DJ15) under salt-stress field conditions from the population of a salt tolerant Dongxiang wild rice × a cultivated rice variety Ningjing16 (NJ16). Genomic resequencing analysis of NJ16, DJ15 and Dongxiang wild rice revealed that the introgressed genomic fragments were unevenly distributed over the 12 chromosomes (Chr.) and mainly identified on Chr. 6, 7, 10, and 11. Using quantitative trait locus (QTL) mapping, we found 9 QTL for salt tolerance (qST) at the seedling stage located on Chr. 1, 3, 4, 5, 6, 8, and 10. In addition, sequence variant analysis within the QTL regions demonstrated that SKC1/HKT8/HKT1;5 and HAK6 transporters along with numerous transcriptional factors were the candidate genes for the salt tolerant QTL. The DJ15/Koshihikari recombinant inbred lines that contained both qST1.2 and qST6, two QTL with the highest effect for salt tolerance, were more tolerant than the parental lines under salt-stress field conditions. Furthermore, the qST6 near-isogenic lines with IR29 background were more tolerant than IR29, indicating that qST1.2 and qST6 could improve salt tolerance in rice. Overall, our study indicates that wild rice genes could markedly improve the salt tolerance of cultivated rice. |
topic |
wild rice cultivated rice salt tolerance QTL gene introgression |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.02269/full |
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