Expression profiling of the Dof gene family under abiotic stresses in spinach
Abstract DNA-binding with one finger (Dof) are plant-specific transcription factors involved in numerous pathways of plant development, such as abiotic stresses responses. Although genome-wide analysis of Dof genes has been performed in many species, but these genes in spinach have not been analyzed...
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doaj-e44cf7a2436c4546a6eed8b4bad070d42021-07-18T11:26:58ZengNature Publishing GroupScientific Reports2045-23222021-07-0111111310.1038/s41598-021-93383-6Expression profiling of the Dof gene family under abiotic stresses in spinachHongying Yu0Yaying Ma1Yijing Lu2Jingjing Yue3Ray Ming4College of Agriculture, Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry UniversityCollege of Agriculture, Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry UniversityCollege of Agriculture, Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry UniversityCenter for Genomics and Biotechnology, Fujian Agriculture and Forestry UniversityDepartment of Plant Biology, University of Illinois at Urbana-ChampaignAbstract DNA-binding with one finger (Dof) are plant-specific transcription factors involved in numerous pathways of plant development, such as abiotic stresses responses. Although genome-wide analysis of Dof genes has been performed in many species, but these genes in spinach have not been analyzed yet. We performed a genome-wide analysis and characterization of Dof gene family in spinach (Spinacia oleracea L.). Twenty-two Dof genes were identified and classified into four groups with nine subgroups, which was further corroborated by gene structure and motif analyses. Ka/Ks analysis revealed that SoDofs were subjected to purifying selection. Using cis-acting elements analysis, SoDofs were involved in plant growth and development, plant hormones, and stress responses. Expression profiling demonstrated that SoDofs expressed in leaf and inflorescence, and responded to cold, heat, and drought stresses. SoDof22 expressed the highest level in male flowers and under cold stress. These results provided a genome-wide analysis of SoDof genes, their gender- and tissue-specific expression, and response to abiotic stresses. The knowledge and resources gained from these analyses will benefit spinach improvement.https://doi.org/10.1038/s41598-021-93383-6 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hongying Yu Yaying Ma Yijing Lu Jingjing Yue Ray Ming |
spellingShingle |
Hongying Yu Yaying Ma Yijing Lu Jingjing Yue Ray Ming Expression profiling of the Dof gene family under abiotic stresses in spinach Scientific Reports |
author_facet |
Hongying Yu Yaying Ma Yijing Lu Jingjing Yue Ray Ming |
author_sort |
Hongying Yu |
title |
Expression profiling of the Dof gene family under abiotic stresses in spinach |
title_short |
Expression profiling of the Dof gene family under abiotic stresses in spinach |
title_full |
Expression profiling of the Dof gene family under abiotic stresses in spinach |
title_fullStr |
Expression profiling of the Dof gene family under abiotic stresses in spinach |
title_full_unstemmed |
Expression profiling of the Dof gene family under abiotic stresses in spinach |
title_sort |
expression profiling of the dof gene family under abiotic stresses in spinach |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-07-01 |
description |
Abstract DNA-binding with one finger (Dof) are plant-specific transcription factors involved in numerous pathways of plant development, such as abiotic stresses responses. Although genome-wide analysis of Dof genes has been performed in many species, but these genes in spinach have not been analyzed yet. We performed a genome-wide analysis and characterization of Dof gene family in spinach (Spinacia oleracea L.). Twenty-two Dof genes were identified and classified into four groups with nine subgroups, which was further corroborated by gene structure and motif analyses. Ka/Ks analysis revealed that SoDofs were subjected to purifying selection. Using cis-acting elements analysis, SoDofs were involved in plant growth and development, plant hormones, and stress responses. Expression profiling demonstrated that SoDofs expressed in leaf and inflorescence, and responded to cold, heat, and drought stresses. SoDof22 expressed the highest level in male flowers and under cold stress. These results provided a genome-wide analysis of SoDof genes, their gender- and tissue-specific expression, and response to abiotic stresses. The knowledge and resources gained from these analyses will benefit spinach improvement. |
url |
https://doi.org/10.1038/s41598-021-93383-6 |
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