Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China

Abstract Background Rattus norvegicus and Suncus murinus are important reservoirs of zoonotic bacterial diseases. An understanding of the composition of gut and oropharynx bacteria in these animals is important for monitoring and preventing such diseases. We therefore examined gut and oropharynx bac...

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Main Authors: Wen-qiao He, Yi-quan Xiong, Jing Ge, Yan-xia Chen, Xue-jiao Chen, Xue-shan Zhong, Ze-jin Ou, Yu-han Gao, Ming-ji Cheng, Yun Mo, Yu-qi Wen, Min Qiu, Shu-ting Huo, Shao-wei Chen, Xue-yan Zheng, Huan He, Yong-zhi Li, Fang-fei You, Min-yi Zhang, Qing Chen
Format: Article
Language:English
Published: BMC 2020-10-01
Series:BMC Veterinary Research
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12917-020-02619-6
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language English
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author Wen-qiao He
Yi-quan Xiong
Jing Ge
Yan-xia Chen
Xue-jiao Chen
Xue-shan Zhong
Ze-jin Ou
Yu-han Gao
Ming-ji Cheng
Yun Mo
Yu-qi Wen
Min Qiu
Shu-ting Huo
Shao-wei Chen
Xue-yan Zheng
Huan He
Yong-zhi Li
Fang-fei You
Min-yi Zhang
Qing Chen
spellingShingle Wen-qiao He
Yi-quan Xiong
Jing Ge
Yan-xia Chen
Xue-jiao Chen
Xue-shan Zhong
Ze-jin Ou
Yu-han Gao
Ming-ji Cheng
Yun Mo
Yu-qi Wen
Min Qiu
Shu-ting Huo
Shao-wei Chen
Xue-yan Zheng
Huan He
Yong-zhi Li
Fang-fei You
Min-yi Zhang
Qing Chen
Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
BMC Veterinary Research
Rattus norvegicus
Suncus murinus
Bacterial composition
Next-generation sequencing
author_facet Wen-qiao He
Yi-quan Xiong
Jing Ge
Yan-xia Chen
Xue-jiao Chen
Xue-shan Zhong
Ze-jin Ou
Yu-han Gao
Ming-ji Cheng
Yun Mo
Yu-qi Wen
Min Qiu
Shu-ting Huo
Shao-wei Chen
Xue-yan Zheng
Huan He
Yong-zhi Li
Fang-fei You
Min-yi Zhang
Qing Chen
author_sort Wen-qiao He
title Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
title_short Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
title_full Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
title_fullStr Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
title_full_unstemmed Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
title_sort composition of gut and oropharynx bacterial communities in rattus norvegicus and suncus murinus in china
publisher BMC
series BMC Veterinary Research
issn 1746-6148
publishDate 2020-10-01
description Abstract Background Rattus norvegicus and Suncus murinus are important reservoirs of zoonotic bacterial diseases. An understanding of the composition of gut and oropharynx bacteria in these animals is important for monitoring and preventing such diseases. We therefore examined gut and oropharynx bacterial composition in these animals in China. Results Proteobacteria, Firmicutes and Bacteroidetes were the most abundant phyla in faecal and throat swab samples of both animals. However, the composition of the bacterial community differed significantly between sample types and animal species. Firmicutes exhibited the highest relative abundance in throat swab samples of R. norvegicus, followed by Proteobacteria and Bacteroidetes. In throat swab specimens of S. murinus, Proteobacteria was the predominant phylum, followed by Firmicutes and Bacteroidetes. Firmicutes showed the highest relative abundance in faecal specimens of R. norvegicus, followed by Bacteroidetes and Proteobacteria. Firmicutes and Proteobacteria had almost equal abundance in faecal specimens of S. murinus, with Bacteroidetes accounting for only 3.07%. The family Streptococcaceae was most common in throat swab samples of R. norvegicus, while Prevotellaceae was most common in its faecal samples. Pseudomonadaceae was the predominant family in throat swab samples of S. murinus, while Enterobacteriaceae was most common in faecal samples. We annotated 33.28% sequences from faecal samples of S. murinus as potential human pathogenic bacteria, approximately 3.06-fold those in R. norvegicus. Potential pathogenic bacteria annotated in throat swab samples of S. murinus were 1.35-fold those in R. norvegicus. Conclusions Bacterial composition of throat swabs and faecal samples from R. norvegicus differed from those of S. murinus. Both species carried various pathogenic bacteria, therefore both should be closely monitored in the future, especially for S. murinus.
topic Rattus norvegicus
Suncus murinus
Bacterial composition
Next-generation sequencing
url http://link.springer.com/article/10.1186/s12917-020-02619-6
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spelling doaj-e46b724868ae4b5a9e6391cf817352742020-11-25T03:52:47ZengBMCBMC Veterinary Research1746-61482020-10-0116111110.1186/s12917-020-02619-6Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in ChinaWen-qiao He0Yi-quan Xiong1Jing Ge2Yan-xia Chen3Xue-jiao Chen4Xue-shan Zhong5Ze-jin Ou6Yu-han Gao7Ming-ji Cheng8Yun Mo9Yu-qi Wen10Min Qiu11Shu-ting Huo12Shao-wei Chen13Xue-yan Zheng14Huan He15Yong-zhi Li16Fang-fei You17Min-yi Zhang18Qing Chen19Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityAbstract Background Rattus norvegicus and Suncus murinus are important reservoirs of zoonotic bacterial diseases. An understanding of the composition of gut and oropharynx bacteria in these animals is important for monitoring and preventing such diseases. We therefore examined gut and oropharynx bacterial composition in these animals in China. Results Proteobacteria, Firmicutes and Bacteroidetes were the most abundant phyla in faecal and throat swab samples of both animals. However, the composition of the bacterial community differed significantly between sample types and animal species. Firmicutes exhibited the highest relative abundance in throat swab samples of R. norvegicus, followed by Proteobacteria and Bacteroidetes. In throat swab specimens of S. murinus, Proteobacteria was the predominant phylum, followed by Firmicutes and Bacteroidetes. Firmicutes showed the highest relative abundance in faecal specimens of R. norvegicus, followed by Bacteroidetes and Proteobacteria. Firmicutes and Proteobacteria had almost equal abundance in faecal specimens of S. murinus, with Bacteroidetes accounting for only 3.07%. The family Streptococcaceae was most common in throat swab samples of R. norvegicus, while Prevotellaceae was most common in its faecal samples. Pseudomonadaceae was the predominant family in throat swab samples of S. murinus, while Enterobacteriaceae was most common in faecal samples. We annotated 33.28% sequences from faecal samples of S. murinus as potential human pathogenic bacteria, approximately 3.06-fold those in R. norvegicus. Potential pathogenic bacteria annotated in throat swab samples of S. murinus were 1.35-fold those in R. norvegicus. Conclusions Bacterial composition of throat swabs and faecal samples from R. norvegicus differed from those of S. murinus. Both species carried various pathogenic bacteria, therefore both should be closely monitored in the future, especially for S. murinus.http://link.springer.com/article/10.1186/s12917-020-02619-6Rattus norvegicusSuncus murinusBacterial compositionNext-generation sequencing