Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
Abstract Background Rattus norvegicus and Suncus murinus are important reservoirs of zoonotic bacterial diseases. An understanding of the composition of gut and oropharynx bacteria in these animals is important for monitoring and preventing such diseases. We therefore examined gut and oropharynx bac...
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2020-10-01
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Online Access: | http://link.springer.com/article/10.1186/s12917-020-02619-6 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen-qiao He Yi-quan Xiong Jing Ge Yan-xia Chen Xue-jiao Chen Xue-shan Zhong Ze-jin Ou Yu-han Gao Ming-ji Cheng Yun Mo Yu-qi Wen Min Qiu Shu-ting Huo Shao-wei Chen Xue-yan Zheng Huan He Yong-zhi Li Fang-fei You Min-yi Zhang Qing Chen |
spellingShingle |
Wen-qiao He Yi-quan Xiong Jing Ge Yan-xia Chen Xue-jiao Chen Xue-shan Zhong Ze-jin Ou Yu-han Gao Ming-ji Cheng Yun Mo Yu-qi Wen Min Qiu Shu-ting Huo Shao-wei Chen Xue-yan Zheng Huan He Yong-zhi Li Fang-fei You Min-yi Zhang Qing Chen Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China BMC Veterinary Research Rattus norvegicus Suncus murinus Bacterial composition Next-generation sequencing |
author_facet |
Wen-qiao He Yi-quan Xiong Jing Ge Yan-xia Chen Xue-jiao Chen Xue-shan Zhong Ze-jin Ou Yu-han Gao Ming-ji Cheng Yun Mo Yu-qi Wen Min Qiu Shu-ting Huo Shao-wei Chen Xue-yan Zheng Huan He Yong-zhi Li Fang-fei You Min-yi Zhang Qing Chen |
author_sort |
Wen-qiao He |
title |
Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China |
title_short |
Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China |
title_full |
Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China |
title_fullStr |
Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China |
title_full_unstemmed |
Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China |
title_sort |
composition of gut and oropharynx bacterial communities in rattus norvegicus and suncus murinus in china |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2020-10-01 |
description |
Abstract Background Rattus norvegicus and Suncus murinus are important reservoirs of zoonotic bacterial diseases. An understanding of the composition of gut and oropharynx bacteria in these animals is important for monitoring and preventing such diseases. We therefore examined gut and oropharynx bacterial composition in these animals in China. Results Proteobacteria, Firmicutes and Bacteroidetes were the most abundant phyla in faecal and throat swab samples of both animals. However, the composition of the bacterial community differed significantly between sample types and animal species. Firmicutes exhibited the highest relative abundance in throat swab samples of R. norvegicus, followed by Proteobacteria and Bacteroidetes. In throat swab specimens of S. murinus, Proteobacteria was the predominant phylum, followed by Firmicutes and Bacteroidetes. Firmicutes showed the highest relative abundance in faecal specimens of R. norvegicus, followed by Bacteroidetes and Proteobacteria. Firmicutes and Proteobacteria had almost equal abundance in faecal specimens of S. murinus, with Bacteroidetes accounting for only 3.07%. The family Streptococcaceae was most common in throat swab samples of R. norvegicus, while Prevotellaceae was most common in its faecal samples. Pseudomonadaceae was the predominant family in throat swab samples of S. murinus, while Enterobacteriaceae was most common in faecal samples. We annotated 33.28% sequences from faecal samples of S. murinus as potential human pathogenic bacteria, approximately 3.06-fold those in R. norvegicus. Potential pathogenic bacteria annotated in throat swab samples of S. murinus were 1.35-fold those in R. norvegicus. Conclusions Bacterial composition of throat swabs and faecal samples from R. norvegicus differed from those of S. murinus. Both species carried various pathogenic bacteria, therefore both should be closely monitored in the future, especially for S. murinus. |
topic |
Rattus norvegicus Suncus murinus Bacterial composition Next-generation sequencing |
url |
http://link.springer.com/article/10.1186/s12917-020-02619-6 |
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doaj-e46b724868ae4b5a9e6391cf817352742020-11-25T03:52:47ZengBMCBMC Veterinary Research1746-61482020-10-0116111110.1186/s12917-020-02619-6Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in ChinaWen-qiao He0Yi-quan Xiong1Jing Ge2Yan-xia Chen3Xue-jiao Chen4Xue-shan Zhong5Ze-jin Ou6Yu-han Gao7Ming-ji Cheng8Yun Mo9Yu-qi Wen10Min Qiu11Shu-ting Huo12Shao-wei Chen13Xue-yan Zheng14Huan He15Yong-zhi Li16Fang-fei You17Min-yi Zhang18Qing Chen19Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityAbstract Background Rattus norvegicus and Suncus murinus are important reservoirs of zoonotic bacterial diseases. An understanding of the composition of gut and oropharynx bacteria in these animals is important for monitoring and preventing such diseases. We therefore examined gut and oropharynx bacterial composition in these animals in China. Results Proteobacteria, Firmicutes and Bacteroidetes were the most abundant phyla in faecal and throat swab samples of both animals. However, the composition of the bacterial community differed significantly between sample types and animal species. Firmicutes exhibited the highest relative abundance in throat swab samples of R. norvegicus, followed by Proteobacteria and Bacteroidetes. In throat swab specimens of S. murinus, Proteobacteria was the predominant phylum, followed by Firmicutes and Bacteroidetes. Firmicutes showed the highest relative abundance in faecal specimens of R. norvegicus, followed by Bacteroidetes and Proteobacteria. Firmicutes and Proteobacteria had almost equal abundance in faecal specimens of S. murinus, with Bacteroidetes accounting for only 3.07%. The family Streptococcaceae was most common in throat swab samples of R. norvegicus, while Prevotellaceae was most common in its faecal samples. Pseudomonadaceae was the predominant family in throat swab samples of S. murinus, while Enterobacteriaceae was most common in faecal samples. We annotated 33.28% sequences from faecal samples of S. murinus as potential human pathogenic bacteria, approximately 3.06-fold those in R. norvegicus. Potential pathogenic bacteria annotated in throat swab samples of S. murinus were 1.35-fold those in R. norvegicus. Conclusions Bacterial composition of throat swabs and faecal samples from R. norvegicus differed from those of S. murinus. Both species carried various pathogenic bacteria, therefore both should be closely monitored in the future, especially for S. murinus.http://link.springer.com/article/10.1186/s12917-020-02619-6Rattus norvegicusSuncus murinusBacterial compositionNext-generation sequencing |