Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of <i>Allium Mongolicum</i> Regel and Their Intestinal Motility Evaluation

• Main Authors: Yongzhe Dong, Jingya Ruan, Zhijuan Ding, Wei Zhao, Mimi Hao, Ying Zhang, Hongyu Jiang, Yi Zhang, Tao Wang
• Format: Article
• Language: English
• Published: MDPI AG 2020-01-01
• Series: Molecules
• Subjects: <i>allium mongolicum</i> regel; flavonoids; phenolic acids; mouse isolated intestine tissue; qualitative analysis
• Online Access: https://www.mdpi.com/1420-3049/25/3/577

To clarify whether flavonoids and phenols in <i>Allium mongolicum</i> Regel have the effect of improving gastrointestinal function and analyze its quality, this study was designed to isolate and identify them from the aerial parts of <i>A. mongolicum</i> by using various chromatographic and spectrophotometric methods, a bioassay on motility of mouse isolated intestine tissue, as well as qualitative analysis using liquid chromatography/mass spectrometry (LC-MS) analysis. As a result, 31 flavonoids and phenolic acids were obtained and identified, including six new flavonoid glycosides, mongoflavonosides A₁ (<b>1</b>), A₂ (<b>2</b>), A₃ (<b>3</b>), A₄ (<b>4</b>), B₁ (<b>5</b>), B₂ (<b>6</b>), and four new phenolic acid glycosides, mongophenosides A₁ (<b>7</b>), A₂ (<b>8</b>), A₃ (<b>9</b>), B (<b>10</b>). Among them, eleven flavonoids and three phenolic acids showed significant increase in the height of mouse small intestinal muscle. It was a first systematic bioactive constituents&#8217; study for <i>A. mongolicum</i> on gastrointestinal tract. Furthermore, according to the retention time (<i>t</i>_R) and the exact mass-to-charge ratio (<i>m/z</i>), thirty-one compounds were unambiguously identified by comparing to the standard references by using LC-MS. Then, on the basis of generalized rules of MS/MS fragmentation pattern, chromatographic behaviors, as well as biosynthetic laws of the 31 isolates, five flavonoid glycosides and one phenolic acid glycoside were tentatively speculated. On the basis of the study, a fast analysis method for flavonoids and phenolic acids in <i>A. mongolicum</i> was established.