The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum.
The mitochondrial genome in the human malaria parasite Plasmodium falciparum is most unusual. Over half the genome is composed of the genes for three classic mitochondrial proteins: cytochrome oxidase subunits I and III and apocytochrome b. The remainder encodes numerous small RNAs, ranging in size...
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doaj-e4730cdfb5f14681b48d38d4787c1a8b2020-11-24T22:25:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3832010.1371/journal.pone.0038320The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum.Jean E FeaginMaria Isabel HarrellJung C LeeKevin J CoeBryan H SandsJamie J CannoneGermaine TamiMurray N SchnareRobin R GutellThe mitochondrial genome in the human malaria parasite Plasmodium falciparum is most unusual. Over half the genome is composed of the genes for three classic mitochondrial proteins: cytochrome oxidase subunits I and III and apocytochrome b. The remainder encodes numerous small RNAs, ranging in size from 23 to 190 nt. Previous analysis revealed that some of these transcripts have significant sequence identity with highly conserved regions of large and small subunit rRNAs, and can form the expected secondary structures. However, these rRNA fragments are not encoded in linear order; instead, they are intermixed with one another and the protein coding genes, and are coded on both strands of the genome. This unorthodox arrangement hindered the identification of transcripts corresponding to other regions of rRNA that are highly conserved and/or are known to participate directly in protein synthesis.The identification of 14 additional small mitochondrial transcripts from P. falciparum and the assignment of 27 small RNAs (12 SSU RNAs totaling 804 nt, 15 LSU RNAs totaling 1233 nt) to specific regions of rRNA are supported by multiple lines of evidence. The regions now represented are highly similar to those of the small but contiguous mitochondrial rRNAs of Caenorhabditis elegans. The P. falciparum rRNA fragments cluster on the interfaces of the two ribosomal subunits in the three-dimensional structure of the ribosome.All of the rRNA fragments are now presumed to have been identified with experimental methods, and nearly all of these have been mapped onto the SSU and LSU rRNAs. Conversely, all regions of the rRNAs that are known to be directly associated with protein synthesis have been identified in the P. falciparum mitochondrial genome and RNA transcripts. The fragmentation of the rRNA in the P. falciparum mitochondrion is the most extreme example of any rRNA fragmentation discovered.http://europepmc.org/articles/PMC3382252?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jean E Feagin Maria Isabel Harrell Jung C Lee Kevin J Coe Bryan H Sands Jamie J Cannone Germaine Tami Murray N Schnare Robin R Gutell |
spellingShingle |
Jean E Feagin Maria Isabel Harrell Jung C Lee Kevin J Coe Bryan H Sands Jamie J Cannone Germaine Tami Murray N Schnare Robin R Gutell The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum. PLoS ONE |
author_facet |
Jean E Feagin Maria Isabel Harrell Jung C Lee Kevin J Coe Bryan H Sands Jamie J Cannone Germaine Tami Murray N Schnare Robin R Gutell |
author_sort |
Jean E Feagin |
title |
The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum. |
title_short |
The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum. |
title_full |
The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum. |
title_fullStr |
The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum. |
title_full_unstemmed |
The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum. |
title_sort |
fragmented mitochondrial ribosomal rnas of plasmodium falciparum. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
The mitochondrial genome in the human malaria parasite Plasmodium falciparum is most unusual. Over half the genome is composed of the genes for three classic mitochondrial proteins: cytochrome oxidase subunits I and III and apocytochrome b. The remainder encodes numerous small RNAs, ranging in size from 23 to 190 nt. Previous analysis revealed that some of these transcripts have significant sequence identity with highly conserved regions of large and small subunit rRNAs, and can form the expected secondary structures. However, these rRNA fragments are not encoded in linear order; instead, they are intermixed with one another and the protein coding genes, and are coded on both strands of the genome. This unorthodox arrangement hindered the identification of transcripts corresponding to other regions of rRNA that are highly conserved and/or are known to participate directly in protein synthesis.The identification of 14 additional small mitochondrial transcripts from P. falciparum and the assignment of 27 small RNAs (12 SSU RNAs totaling 804 nt, 15 LSU RNAs totaling 1233 nt) to specific regions of rRNA are supported by multiple lines of evidence. The regions now represented are highly similar to those of the small but contiguous mitochondrial rRNAs of Caenorhabditis elegans. The P. falciparum rRNA fragments cluster on the interfaces of the two ribosomal subunits in the three-dimensional structure of the ribosome.All of the rRNA fragments are now presumed to have been identified with experimental methods, and nearly all of these have been mapped onto the SSU and LSU rRNAs. Conversely, all regions of the rRNAs that are known to be directly associated with protein synthesis have been identified in the P. falciparum mitochondrial genome and RNA transcripts. The fragmentation of the rRNA in the P. falciparum mitochondrion is the most extreme example of any rRNA fragmentation discovered. |
url |
http://europepmc.org/articles/PMC3382252?pdf=render |
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