Effect of prenatal immobilization stress on spatial memory, anxiety-like behavior and brain BDNF concentration in the F1 generation male mice
Background and Aim: In this study, we investigated the effect of immobilization stress during pregnancy on the spatial memory, anxiety-like behavior and brain BDNF level in F1 generation male NMRI mice. Materials and Methods: Twenty female pregnant mice were randomly divided into stress and cont...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | fas |
Published: |
Kurdistan University of Medical Sciences
2018-08-01
|
Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
Subjects: | |
Online Access: | http://sjku.muk.ac.ir/article-1-4269-en.pdf |
Summary: | Background and Aim: In this study, we investigated the effect of immobilization stress
during pregnancy on the spatial memory, anxiety-like behavior and brain BDNF level in F1
generation male NMRI mice.
Materials and Methods: Twenty female pregnant mice were randomly divided into stress
and control groups. The stress group received stress using a restraint cylinder (6 cm ID, 20 cm
L) 60 min/day from the 1st to the 15th day of pregnancy. The control group did not receive
stress. The male F1 offspring was nursed by their mothers (n=10/group) until reaching weights
between 20 and 25 g. Then they were tested for spatial memory using Barnes maze and
anxiety-like behavior by the elevated plus-maze. Also, brain BDNF level was measured by
means of an ELISA reader.
Results: Barnes maze test results showed that the time and distance to reach the target hole
were significantly (p<0.001) increased in the stress group. Also, the number of errors and
anxiety-like behavior in the stress group were significantly (p<0.001) increased compared to
those in the control group (p<0.001). BDNF level in the brain was significantly (p<0.05)
higher in the control group.
Conclusion: The present study indicated that prenatal stress can lead to decreased level of
BDNF in the brain of the offspring which may result in spatial memory disorder and anxietylike behavior. |
---|---|
ISSN: | 1560-652X 2345-4040 |