Nox4 Maintains Blood Pressure during Low Sodium Diet
The NADPH oxidase Nox4 is a hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-producing enzyme, with the highest expression in the kidney. As the kidney is involved in volume and blood pressure control through sodium handling, we set out to determine the impact of a low sodium d...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-07-01
|
Series: | Antioxidants |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-3921/10/7/1103 |
id |
doaj-e4be28dfc22642af9934e88fbb2172fa |
---|---|
record_format |
Article |
spelling |
doaj-e4be28dfc22642af9934e88fbb2172fa2021-07-23T13:28:46ZengMDPI AGAntioxidants2076-39212021-07-01101103110310.3390/antiox10071103Nox4 Maintains Blood Pressure during Low Sodium DietFlávia Rezende0Pedro Felipe Malacarne1Niklas Müller2Birgit Rathkolb3Martin Hrabě de Angelis4Katrin Schröder5Ralf P Brandes6Institute for Cardiovascular Physiology, Goethe University, Theodor-Stern Kai 7, 60590 Frankfurt, GermanyInstitute for Cardiovascular Physiology, Goethe University, Theodor-Stern Kai 7, 60590 Frankfurt, GermanyInstitute for Cardiovascular Physiology, Goethe University, Theodor-Stern Kai 7, 60590 Frankfurt, GermanyInstitute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstaedter Landstr. 1, 85764 Neuherberg, GermanyInstitute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstaedter Landstr. 1, 85764 Neuherberg, GermanyInstitute for Cardiovascular Physiology, Goethe University, Theodor-Stern Kai 7, 60590 Frankfurt, GermanyInstitute for Cardiovascular Physiology, Goethe University, Theodor-Stern Kai 7, 60590 Frankfurt, GermanyThe NADPH oxidase Nox4 is a hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-producing enzyme, with the highest expression in the kidney. As the kidney is involved in volume and blood pressure control through sodium handling, we set out to determine the impact of a low sodium diet on these parameters in WT and Nox4-/- mice. Nox4 expression in the murine kidney was restricted to the proximal tubule. Nevertheless, low-sodium-induced weight loss and sodium sparing function was similar in WT and Nox4-/- mice, disputing an important function of renal Nox4 in sodium handling. In contrast, a low sodium diet resulted in a reduction in systolic blood pressure in Nox4-/- as compared to WT mice. This was associated with a selectively lower pressure to heart-rate ratio, as well as heart to body weight ratio. In general, a low sodium diet leads to activation of sympathetic tone and the renin angiotensin system, which subsequently increases peripheral resistance. Our observations suggest that the control by this system is attenuated in Nox4-/- mice, resulting in lower blood pressure in response to low sodium.https://www.mdpi.com/2076-3921/10/7/1103NADPH oxidase 4proximal tubule cellsreactive oxygen species |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Flávia Rezende Pedro Felipe Malacarne Niklas Müller Birgit Rathkolb Martin Hrabě de Angelis Katrin Schröder Ralf P Brandes |
spellingShingle |
Flávia Rezende Pedro Felipe Malacarne Niklas Müller Birgit Rathkolb Martin Hrabě de Angelis Katrin Schröder Ralf P Brandes Nox4 Maintains Blood Pressure during Low Sodium Diet Antioxidants NADPH oxidase 4 proximal tubule cells reactive oxygen species |
author_facet |
Flávia Rezende Pedro Felipe Malacarne Niklas Müller Birgit Rathkolb Martin Hrabě de Angelis Katrin Schröder Ralf P Brandes |
author_sort |
Flávia Rezende |
title |
Nox4 Maintains Blood Pressure during Low Sodium Diet |
title_short |
Nox4 Maintains Blood Pressure during Low Sodium Diet |
title_full |
Nox4 Maintains Blood Pressure during Low Sodium Diet |
title_fullStr |
Nox4 Maintains Blood Pressure during Low Sodium Diet |
title_full_unstemmed |
Nox4 Maintains Blood Pressure during Low Sodium Diet |
title_sort |
nox4 maintains blood pressure during low sodium diet |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2021-07-01 |
description |
The NADPH oxidase Nox4 is a hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-producing enzyme, with the highest expression in the kidney. As the kidney is involved in volume and blood pressure control through sodium handling, we set out to determine the impact of a low sodium diet on these parameters in WT and Nox4-/- mice. Nox4 expression in the murine kidney was restricted to the proximal tubule. Nevertheless, low-sodium-induced weight loss and sodium sparing function was similar in WT and Nox4-/- mice, disputing an important function of renal Nox4 in sodium handling. In contrast, a low sodium diet resulted in a reduction in systolic blood pressure in Nox4-/- as compared to WT mice. This was associated with a selectively lower pressure to heart-rate ratio, as well as heart to body weight ratio. In general, a low sodium diet leads to activation of sympathetic tone and the renin angiotensin system, which subsequently increases peripheral resistance. Our observations suggest that the control by this system is attenuated in Nox4-/- mice, resulting in lower blood pressure in response to low sodium. |
topic |
NADPH oxidase 4 proximal tubule cells reactive oxygen species |
url |
https://www.mdpi.com/2076-3921/10/7/1103 |
work_keys_str_mv |
AT flaviarezende nox4maintainsbloodpressureduringlowsodiumdiet AT pedrofelipemalacarne nox4maintainsbloodpressureduringlowsodiumdiet AT niklasmuller nox4maintainsbloodpressureduringlowsodiumdiet AT birgitrathkolb nox4maintainsbloodpressureduringlowsodiumdiet AT martinhrabedeangelis nox4maintainsbloodpressureduringlowsodiumdiet AT katrinschroder nox4maintainsbloodpressureduringlowsodiumdiet AT ralfpbrandes nox4maintainsbloodpressureduringlowsodiumdiet |
_version_ |
1721289731596091392 |