The complete mitochondrial genome of Lerema accius and its phylogenetic implications

The complete mitochondrial genome of Lerema accius and its phylogenetic implications

Butterflies and moths (Lepidoptera) are becoming model organisms for genetics and evolutionary biology. Decoding the Lepidoptera genomes, both nuclear and mitochondrial, is an essential step in these studies. Here we describe a protocol to assemble mitogenomes from Next Generation Sequencing reads o...

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Bibliographic Details
Main Authors: Qian Cong, Nick V. Grishin
Format: Article
Language:English
Published: PeerJ Inc. 2016-01-01
Series:PeerJ
Subjects:
Illumina sequencing
Clouded Skipper
De novo assembly
Hesperiinae
Online Access:https://peerj.com/articles/1546.pdf
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spelling doaj-e4c044e6a6c2467592d5f7a8c8b01e362020-11-25T00:31:56ZengPeerJ Inc.PeerJ2167-83592016-01-014e154610.7717/peerj.1546The complete mitochondrial genome of Lerema accius and its phylogenetic implicationsQian Cong0Nick V. Grishin1Departments of Biophysics and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX, United StatesDepartments of Biophysics and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX, United StatesButterflies and moths (Lepidoptera) are becoming model organisms for genetics and evolutionary biology. Decoding the Lepidoptera genomes, both nuclear and mitochondrial, is an essential step in these studies. Here we describe a protocol to assemble mitogenomes from Next Generation Sequencing reads obtained through whole-genome sequencing and report the 15,338 bp mitogenome of Lerema accius. The mitogenome is AT-rich and encodes 13 proteins, 22 transfer-RNAs, and two ribosomal-RNAs, with a gene order typical for Lepidoptera mitogenomes. A phylogenetic study based on the protein sequences using both Bayesian Inference and Maximum Likelihood methods consistently place Lerema accius with other grass skippers (Hesperiinae).https://peerj.com/articles/1546.pdfIllumina sequencingClouded SkipperDe novo assemblyHesperiinae
collection DOAJ
language English
format Article
sources DOAJ
author Qian Cong
Nick V. Grishin
spellingShingle Qian Cong
Nick V. Grishin
The complete mitochondrial genome of Lerema accius and its phylogenetic implications
PeerJ
Illumina sequencing
Clouded Skipper
De novo assembly
Hesperiinae
author_facet Qian Cong
Nick V. Grishin
author_sort Qian Cong
title The complete mitochondrial genome of Lerema accius and its phylogenetic implications
title_short The complete mitochondrial genome of Lerema accius and its phylogenetic implications
title_full The complete mitochondrial genome of Lerema accius and its phylogenetic implications
title_fullStr The complete mitochondrial genome of Lerema accius and its phylogenetic implications
title_full_unstemmed The complete mitochondrial genome of Lerema accius and its phylogenetic implications
title_sort complete mitochondrial genome of lerema accius and its phylogenetic implications
publisher PeerJ Inc.
series PeerJ
issn 2167-8359
publishDate 2016-01-01
description Butterflies and moths (Lepidoptera) are becoming model organisms for genetics and evolutionary biology. Decoding the Lepidoptera genomes, both nuclear and mitochondrial, is an essential step in these studies. Here we describe a protocol to assemble mitogenomes from Next Generation Sequencing reads obtained through whole-genome sequencing and report the 15,338 bp mitogenome of Lerema accius. The mitogenome is AT-rich and encodes 13 proteins, 22 transfer-RNAs, and two ribosomal-RNAs, with a gene order typical for Lepidoptera mitogenomes. A phylogenetic study based on the protein sequences using both Bayesian Inference and Maximum Likelihood methods consistently place Lerema accius with other grass skippers (Hesperiinae).
topic Illumina sequencing
Clouded Skipper
De novo assembly
Hesperiinae
url https://peerj.com/articles/1546.pdf
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