Insights into deep-sea sediment fungal communities from the East Indian Ocean using targeted environmental sequencing combined with traditional cultivation.
The fungal diversity in deep-sea environments has recently gained an increasing amount attention. Our knowledge and understanding of the true fungal diversity and the role it plays in deep-sea environments, however, is still limited. We investigated the fungal community structure in five sediments f...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4182876?pdf=render |
id |
doaj-e4d04456dea94ea781b302c00654e86d |
---|---|
record_format |
Article |
spelling |
doaj-e4d04456dea94ea781b302c00654e86d2020-11-25T01:18:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e10911810.1371/journal.pone.0109118Insights into deep-sea sediment fungal communities from the East Indian Ocean using targeted environmental sequencing combined with traditional cultivation.Xiao-yong ZhangGui-ling TangXin-ya XuXu-hua NongShu-hua QiThe fungal diversity in deep-sea environments has recently gained an increasing amount attention. Our knowledge and understanding of the true fungal diversity and the role it plays in deep-sea environments, however, is still limited. We investigated the fungal community structure in five sediments from a depth of ∼ 4000 m in the East India Ocean using a combination of targeted environmental sequencing and traditional cultivation. This approach resulted in the recovery of a total of 45 fungal operational taxonomic units (OTUs) and 20 culturable fungal phylotypes. This finding indicates that there is a great amount of fungal diversity in the deep-sea sediments collected in the East Indian Ocean. Three fungal OTUs and one culturable phylotype demonstrated high divergence (89%-97%) from the existing sequences in the GenBank. Moreover, 44.4% fungal OTUs and 30% culturable fungal phylotypes are new reports for deep-sea sediments. These results suggest that the deep-sea sediments from the East India Ocean can serve as habitats for new fungal communities compared with other deep-sea environments. In addition, different fungal community could be detected when using targeted environmental sequencing compared with traditional cultivation in this study, which suggests that a combination of targeted environmental sequencing or traditional cultivation alone. This study is the first to report new insights into the fungal communities in deep-sea sediments environmental sequencing and traditional cultivation will generate a more diverse fungal community in deep-sea environments than using either from the East Indian Ocean, which increases our knowledge and understanding of the fungal diversity in deep-sea environments.http://europepmc.org/articles/PMC4182876?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao-yong Zhang Gui-ling Tang Xin-ya Xu Xu-hua Nong Shu-hua Qi |
spellingShingle |
Xiao-yong Zhang Gui-ling Tang Xin-ya Xu Xu-hua Nong Shu-hua Qi Insights into deep-sea sediment fungal communities from the East Indian Ocean using targeted environmental sequencing combined with traditional cultivation. PLoS ONE |
author_facet |
Xiao-yong Zhang Gui-ling Tang Xin-ya Xu Xu-hua Nong Shu-hua Qi |
author_sort |
Xiao-yong Zhang |
title |
Insights into deep-sea sediment fungal communities from the East Indian Ocean using targeted environmental sequencing combined with traditional cultivation. |
title_short |
Insights into deep-sea sediment fungal communities from the East Indian Ocean using targeted environmental sequencing combined with traditional cultivation. |
title_full |
Insights into deep-sea sediment fungal communities from the East Indian Ocean using targeted environmental sequencing combined with traditional cultivation. |
title_fullStr |
Insights into deep-sea sediment fungal communities from the East Indian Ocean using targeted environmental sequencing combined with traditional cultivation. |
title_full_unstemmed |
Insights into deep-sea sediment fungal communities from the East Indian Ocean using targeted environmental sequencing combined with traditional cultivation. |
title_sort |
insights into deep-sea sediment fungal communities from the east indian ocean using targeted environmental sequencing combined with traditional cultivation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
The fungal diversity in deep-sea environments has recently gained an increasing amount attention. Our knowledge and understanding of the true fungal diversity and the role it plays in deep-sea environments, however, is still limited. We investigated the fungal community structure in five sediments from a depth of ∼ 4000 m in the East India Ocean using a combination of targeted environmental sequencing and traditional cultivation. This approach resulted in the recovery of a total of 45 fungal operational taxonomic units (OTUs) and 20 culturable fungal phylotypes. This finding indicates that there is a great amount of fungal diversity in the deep-sea sediments collected in the East Indian Ocean. Three fungal OTUs and one culturable phylotype demonstrated high divergence (89%-97%) from the existing sequences in the GenBank. Moreover, 44.4% fungal OTUs and 30% culturable fungal phylotypes are new reports for deep-sea sediments. These results suggest that the deep-sea sediments from the East India Ocean can serve as habitats for new fungal communities compared with other deep-sea environments. In addition, different fungal community could be detected when using targeted environmental sequencing compared with traditional cultivation in this study, which suggests that a combination of targeted environmental sequencing or traditional cultivation alone. This study is the first to report new insights into the fungal communities in deep-sea sediments environmental sequencing and traditional cultivation will generate a more diverse fungal community in deep-sea environments than using either from the East Indian Ocean, which increases our knowledge and understanding of the fungal diversity in deep-sea environments. |
url |
http://europepmc.org/articles/PMC4182876?pdf=render |
work_keys_str_mv |
AT xiaoyongzhang insightsintodeepseasedimentfungalcommunitiesfromtheeastindianoceanusingtargetedenvironmentalsequencingcombinedwithtraditionalcultivation AT guilingtang insightsintodeepseasedimentfungalcommunitiesfromtheeastindianoceanusingtargetedenvironmentalsequencingcombinedwithtraditionalcultivation AT xinyaxu insightsintodeepseasedimentfungalcommunitiesfromtheeastindianoceanusingtargetedenvironmentalsequencingcombinedwithtraditionalcultivation AT xuhuanong insightsintodeepseasedimentfungalcommunitiesfromtheeastindianoceanusingtargetedenvironmentalsequencingcombinedwithtraditionalcultivation AT shuhuaqi insightsintodeepseasedimentfungalcommunitiesfromtheeastindianoceanusingtargetedenvironmentalsequencingcombinedwithtraditionalcultivation |
_version_ |
1725140623270346752 |