Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models.
The utilization of 3D, physiologically relevant in vitro cancer models to investigate complex interactions between tumor and stroma has been increasing. Prior work has generally focused on the cancer cells and, the role of fibroblast culture conditions on tumor-stromal cell interactions is still lar...
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doaj-e4d062b51734496cbc19bffd25142d3c2020-11-24T21:32:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7637310.1371/journal.pone.0076373Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models.Kyung Eun SungXiaojing SuErwin BerthierCarolyn PehlkeAndreas FriedlDavid J BeebeThe utilization of 3D, physiologically relevant in vitro cancer models to investigate complex interactions between tumor and stroma has been increasing. Prior work has generally focused on the cancer cells and, the role of fibroblast culture conditions on tumor-stromal cell interactions is still largely unknown. Here, we focus on the stroma by comparing functional behaviors of human mammary fibroblasts (HMFs) cultured in 2D and 3D and their effects on the invasive progression of breast cancer cells (MCF10DCIS.com). We identified increased levels of several paracrine factors from HMFs cultured in 3D conditions that drive the invasive transition. Using a microscale co-culture model with improved compartmentalization and sensitivity, we demonstrated that HMFs cultured in 3D intensify the promotion of the invasive progression through the HGF/c-Met interaction. This study highlights the importance of the 3D stromal microenvironment in the development of multiple cell type in vitro cancer models.http://europepmc.org/articles/PMC3790689?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kyung Eun Sung Xiaojing Su Erwin Berthier Carolyn Pehlke Andreas Friedl David J Beebe |
spellingShingle |
Kyung Eun Sung Xiaojing Su Erwin Berthier Carolyn Pehlke Andreas Friedl David J Beebe Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models. PLoS ONE |
author_facet |
Kyung Eun Sung Xiaojing Su Erwin Berthier Carolyn Pehlke Andreas Friedl David J Beebe |
author_sort |
Kyung Eun Sung |
title |
Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models. |
title_short |
Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models. |
title_full |
Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models. |
title_fullStr |
Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models. |
title_full_unstemmed |
Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models. |
title_sort |
understanding the impact of 2d and 3d fibroblast cultures on in vitro breast cancer models. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
The utilization of 3D, physiologically relevant in vitro cancer models to investigate complex interactions between tumor and stroma has been increasing. Prior work has generally focused on the cancer cells and, the role of fibroblast culture conditions on tumor-stromal cell interactions is still largely unknown. Here, we focus on the stroma by comparing functional behaviors of human mammary fibroblasts (HMFs) cultured in 2D and 3D and their effects on the invasive progression of breast cancer cells (MCF10DCIS.com). We identified increased levels of several paracrine factors from HMFs cultured in 3D conditions that drive the invasive transition. Using a microscale co-culture model with improved compartmentalization and sensitivity, we demonstrated that HMFs cultured in 3D intensify the promotion of the invasive progression through the HGF/c-Met interaction. This study highlights the importance of the 3D stromal microenvironment in the development of multiple cell type in vitro cancer models. |
url |
http://europepmc.org/articles/PMC3790689?pdf=render |
work_keys_str_mv |
AT kyungeunsung understandingtheimpactof2dand3dfibroblastculturesoninvitrobreastcancermodels AT xiaojingsu understandingtheimpactof2dand3dfibroblastculturesoninvitrobreastcancermodels AT erwinberthier understandingtheimpactof2dand3dfibroblastculturesoninvitrobreastcancermodels AT carolynpehlke understandingtheimpactof2dand3dfibroblastculturesoninvitrobreastcancermodels AT andreasfriedl understandingtheimpactof2dand3dfibroblastculturesoninvitrobreastcancermodels AT davidjbeebe understandingtheimpactof2dand3dfibroblastculturesoninvitrobreastcancermodels |
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