Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles
Nanoparticles (NPs) typically accumulate in lysosomes. However, their impact on lysosomal function, as well as autophagy, a lysosomal degradative pathway, is still not well known. We have previously reported in the 1321N1 cell line that amine-modified polystyrene (NH2-PS) NPs induce apoptosis throug...
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2018-04-01
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doaj-e4d5594687934bfdb56bc4e119441bcb2020-11-25T03:57:02ZengThe Royal SocietyOpen Biology2046-24412018-04-018410.1098/rsob.170271170271Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticlesFengjuan WangAnna SalvatiPatricia BoyaNanoparticles (NPs) typically accumulate in lysosomes. However, their impact on lysosomal function, as well as autophagy, a lysosomal degradative pathway, is still not well known. We have previously reported in the 1321N1 cell line that amine-modified polystyrene (NH2-PS) NPs induce apoptosis through damage initiated in the lysosomes leading ultimately to release of lysosomal content in the cytosol, followed by apoptosis. Here, by using a combination of biochemical and cell biological approaches, we have characterized in a mouse embryonic fibroblast cell line that the lysosomal alterations induced by NH2-PS NPs is progressive, initiating from mild lysosomal membrane permeabilization (LMP), to expansion of lysosomal volume and intensive LMP before the summit of cell death. Though the cells initially seem to induce autophagy as a surviving mechanism, the damage of NH2-PS NPs to lysosomes probably results in lysosomal dysfunctions, leading to blockage of autophagic flux at the level of lysosomes and the eventual cell death.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.170271cationic nanoparticleslysosomal membrane permeabilizationautophagyreactive oxygen speciesnanotoxicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fengjuan Wang Anna Salvati Patricia Boya |
spellingShingle |
Fengjuan Wang Anna Salvati Patricia Boya Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles Open Biology cationic nanoparticles lysosomal membrane permeabilization autophagy reactive oxygen species nanotoxicity |
author_facet |
Fengjuan Wang Anna Salvati Patricia Boya |
author_sort |
Fengjuan Wang |
title |
Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles |
title_short |
Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles |
title_full |
Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles |
title_fullStr |
Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles |
title_full_unstemmed |
Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles |
title_sort |
lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles |
publisher |
The Royal Society |
series |
Open Biology |
issn |
2046-2441 |
publishDate |
2018-04-01 |
description |
Nanoparticles (NPs) typically accumulate in lysosomes. However, their impact on lysosomal function, as well as autophagy, a lysosomal degradative pathway, is still not well known. We have previously reported in the 1321N1 cell line that amine-modified polystyrene (NH2-PS) NPs induce apoptosis through damage initiated in the lysosomes leading ultimately to release of lysosomal content in the cytosol, followed by apoptosis. Here, by using a combination of biochemical and cell biological approaches, we have characterized in a mouse embryonic fibroblast cell line that the lysosomal alterations induced by NH2-PS NPs is progressive, initiating from mild lysosomal membrane permeabilization (LMP), to expansion of lysosomal volume and intensive LMP before the summit of cell death. Though the cells initially seem to induce autophagy as a surviving mechanism, the damage of NH2-PS NPs to lysosomes probably results in lysosomal dysfunctions, leading to blockage of autophagic flux at the level of lysosomes and the eventual cell death. |
topic |
cationic nanoparticles lysosomal membrane permeabilization autophagy reactive oxygen species nanotoxicity |
url |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.170271 |
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