S100A12 and hBD2 Correlate with the Composition of the Fecal Microflora in ELBW Infants and Expansion of E. coli Is Associated with NEC
Objective. To describe the development of the gut microbiota in extremely low birth weight (ELBW) infants with and without necrotizing enterocolitis (NEC) between April 2008 and December 2009, fecal microflora was prospectively analyzed in fecal samples of all ELBW infants using real-time PCR assays...
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Online Access: | http://dx.doi.org/10.1155/2013/150372 |
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doaj-e4dce39f19f94eb4828b9b01abab6eb32020-11-24T21:20:57ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/150372150372S100A12 and hBD2 Correlate with the Composition of the Fecal Microflora in ELBW Infants and Expansion of E. coli Is Associated with NECA. C. Jenke0J. Postberg1B. Mariel2K. Hensel3D. Foell4J. Däbritz5S. Wirth6Department of Neonatology, HELIOS Children’s Hospital, 42283 Wuppertal, Witten/Herdecke University, GermanyDepartment of Neonatology, HELIOS Children’s Hospital, 42283 Wuppertal, Witten/Herdecke University, GermanyDepartment of Neonatology, HELIOS Children’s Hospital, 42283 Wuppertal, Witten/Herdecke University, GermanyDepartment of Neonatology, HELIOS Children’s Hospital, 42283 Wuppertal, Witten/Herdecke University, GermanyDepartment of Pediatric Rheumatology and Immunology, University Children’s Hospital, Münster, GermanyDepartment of Pediatric Rheumatology and Immunology, University Children’s Hospital, Münster, GermanyDepartment of Neonatology, HELIOS Children’s Hospital, 42283 Wuppertal, Witten/Herdecke University, GermanyObjective. To describe the development of the gut microbiota in extremely low birth weight (ELBW) infants with and without necrotizing enterocolitis (NEC) between April 2008 and December 2009, fecal microflora was prospectively analyzed in fecal samples of all ELBW infants using real-time PCR assays. In addition, fecal inflammatory were measured. Results. Fecal microflora established early in ELBW infants and microbiota composition remained stable over the first 28 days of life except for the prevalence of C. difficile which decreased with decreasing antibiotic use. Infants who subsequently developed NEC had an increase of total bacterial count (9.8-fold) 24 h prior to clinical symptoms mainly due to the expansion of E. coli species (21.6-fold), whereas microbiota composition did not differ from healthy ELBW infants five days before onset of NEC. Importantly, S100A12 and hBD2 positively correlated with the total and E. coli bacterial CFU/g feces (r2 0.4 and 0.64, resp.). Conclusions. In summary, we found evidence for a disturbed homeostasis between the intestinal microbiome and host immunity in ELBW infants with NEC. Moreover, S100A12 and hBD2 correlate with the fecal microbiota thus linking the intestinal innate immune response to the bacterial colonization thus possibly providing a diagnostic tool in the future.http://dx.doi.org/10.1155/2013/150372 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
A. C. Jenke J. Postberg B. Mariel K. Hensel D. Foell J. Däbritz S. Wirth |
spellingShingle |
A. C. Jenke J. Postberg B. Mariel K. Hensel D. Foell J. Däbritz S. Wirth S100A12 and hBD2 Correlate with the Composition of the Fecal Microflora in ELBW Infants and Expansion of E. coli Is Associated with NEC BioMed Research International |
author_facet |
A. C. Jenke J. Postberg B. Mariel K. Hensel D. Foell J. Däbritz S. Wirth |
author_sort |
A. C. Jenke |
title |
S100A12 and hBD2 Correlate with the Composition of the Fecal Microflora in ELBW Infants and Expansion of E. coli Is Associated with NEC |
title_short |
S100A12 and hBD2 Correlate with the Composition of the Fecal Microflora in ELBW Infants and Expansion of E. coli Is Associated with NEC |
title_full |
S100A12 and hBD2 Correlate with the Composition of the Fecal Microflora in ELBW Infants and Expansion of E. coli Is Associated with NEC |
title_fullStr |
S100A12 and hBD2 Correlate with the Composition of the Fecal Microflora in ELBW Infants and Expansion of E. coli Is Associated with NEC |
title_full_unstemmed |
S100A12 and hBD2 Correlate with the Composition of the Fecal Microflora in ELBW Infants and Expansion of E. coli Is Associated with NEC |
title_sort |
s100a12 and hbd2 correlate with the composition of the fecal microflora in elbw infants and expansion of e. coli is associated with nec |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
Objective. To describe the development of the gut microbiota in extremely low birth weight (ELBW) infants with and without necrotizing enterocolitis (NEC) between April 2008 and December 2009, fecal microflora was prospectively analyzed in fecal samples of all ELBW infants using real-time PCR assays. In addition, fecal inflammatory were measured. Results. Fecal microflora established early in ELBW infants and microbiota composition remained stable over the first 28 days of life except for the prevalence of C. difficile which decreased with decreasing antibiotic use. Infants who subsequently developed NEC had an increase of total bacterial count (9.8-fold) 24 h prior to clinical symptoms mainly due to the expansion of E. coli species (21.6-fold), whereas microbiota composition did not differ from healthy ELBW infants five days before onset of NEC. Importantly, S100A12 and hBD2 positively correlated with the total and E. coli bacterial CFU/g feces (r2 0.4 and 0.64, resp.). Conclusions. In summary, we found evidence for a disturbed homeostasis between the intestinal microbiome and host immunity in ELBW infants with NEC. Moreover, S100A12 and hBD2 correlate with the fecal microbiota thus linking the intestinal innate immune response to the bacterial colonization thus possibly providing a diagnostic tool in the future. |
url |
http://dx.doi.org/10.1155/2013/150372 |
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